Introduction
Eslicarbazepine acetate (ESL) is a once-daily antiepileptic drug (AED) that is approved for the treatment of partial-onset seizures as monotherapy or adjunctive therapy [
1,
2]. It is thought to act primarily by enhancing slow inactivation of voltage-gated sodium channels [
3]. The efficacy of ESL as adjunctive therapy for partial-onset seizures in adults, together with its safety and tolerability profile in this setting, was established in a series of randomized, double-blind, placebo-controlled, Phase III trials [
4‐
7] and long-term extension studies [
8‐
10]. The efficacy, safety, and tolerability of ESL as monotherapy for the treatment of partial-onset seizures in adults with newly diagnosed epilepsy were established in a Phase III, randomized, double-blind, active-controlled, non-inferiority trial [
11,
12]. In addition, the efficacy, safety, and tolerability of ESL as monotherapy for adults with uncontrolled partial-onset seizures were established in two randomized, Phase III, withdrawal to monotherapy trials [
13,
14].
Although clinical trials are essential in the development and approval of new AEDs, they do not necessarily reflect the effectiveness and tolerability of an agent when used in clinical practice. This is due to clinical trials selecting relatively homogeneous patient populations and typically using rigid dosing and titration schedules, whereas patients encountered in clinical practice have diverse clinical characteristics that necessitate an individualized approach to treatment [
15,
16]. Consequently, there is a need for real-world studies to complement evidence from clinical trials, by elucidating an agent’s effectiveness when used under everyday clinical practice conditions.
The aim of the Euro-Esli study was to conduct an audit of data from clinical practice studies conducted across Europe to establish how the efficacy and tolerability of ESL observed in clinical trials have translated into effectiveness in the real-world setting. The study involved the collaboration of a pan-European group of clinicians and centers, resulting in the largest database of patients treated with ESL in everyday clinical practice to date. The pooling of a large body of data also enabled subgroup analyses to be conducted, which allowed specific aspects of the use of ESL in epilepsy management to be addressed. We present here the primary results of the audit, together with those of some key subgroup analyses.
Methods
Study design
The Euro-Esli study was an exploratory, pooled analysis of data from European clinical practice studies (both prospective and retrospective) that evaluated the effectiveness, safety, and tolerability of ESL as an adjunctive treatment for partial-onset seizures. There were no exclusion criteria for the studies chosen for the analysis in terms of the epilepsy type of the patients studied and/or the number of prior AEDs they had received. Effectiveness was assessed after 3, 6, and 12 months of ESL treatment and at final follow-up. Safety and tolerability were assessed for the duration of ESL treatment. The study protocol was approved by the Ethics Committee of the Hospital Universitario y Politécnico La Fe, Valencia, Spain, as an extension of the local audit.
Study population
Details of the specific inclusion/exclusion criteria used in the individual studies have been published or presented previously [
17‐
29]. The studies included broad inclusion/exclusion criteria, to be representative of the variety of patients encountered in clinical practice.
The current analysis included all patients who initiated ESL for the treatment of epilepsy. Most patients were treated for partial-onset seizures, although patients with generalized seizures were not specifically excluded. However, analyses of effectiveness focussed on partial-onset seizures, with or without secondary generalization. Patients were excluded if their records contained insufficient data for analysis. Duplicate data from patients who were included in more than one study were excluded.
Study assessments
Effectiveness
Assessments of effectiveness comprised: retention, evaluated in terms of retention rate and time to ESL discontinuation; number and type of seizures (total, simple partial, complex partial, and secondarily generalized seizures [
30]); the percentage reduction from baseline in monthly seizure frequency (for total, simple partial, complex partial, and secondarily generalized seizures); responder rate; seizure freedom rate; and the percentage of patients whose seizure frequency remained unchanged and whose seizure frequency worsened, relative to baseline. At baseline (i.e., prior to ESL initiation), monthly seizure frequency was calculated based on the number of seizures experienced during the previous 3 months. At other timepoints, monthly seizure frequency was based on the number of seizures experienced since the previous visit, i.e., during the previous 3 months for the 3- and 6-month visits and during the previous 6 months for the 12-month visit. For the final assessment, monthly seizure frequency was based on the last visit, which could have been at 3, 6, or 12 months; therefore, seizure frequency at the last visit was based on the number of seizures experienced during at least the previous 3 months. Response was defined as ≥50% seizure frequency reduction from baseline and seizure freedom was defined as the occurrence of no seizures since at least the prior visit (either 3 or 6 months).
Safety and tolerability
Safety was assessed by the evaluation of adverse events (AEs) and tolerability was assessed by evaluating the discontinuation of ESL due to AEs. AEs reported by participating clinicians were also classified using the Medical Dictionary for Regulatory Activities version 16.0 [
31]. AEs were classified as being related to ESL treatment if they started after ESL initiation and were considered by the participating clinician(s) to be ESL-related. Certain AEs of special interest were also assessed, which were hyponatremia, psychiatric AEs, and the presence of ESL-related AEs in patients with poor tolerance to previous AEDs.
Additional assessments
Additional assessments included evaluation of information relating to ESL dosing, ESL treatment adherence, and changes to concomitant treatment(s) (pharmacological and non-pharmacological) after initiation of ESL treatment. Information on treatment adherence was collected in clinical charts.
Subgroup analyses
Several subgroup analyses of the pooled data were conducted. These included, first, analysis of data for elderly patients (≥65 years) versus non-elderly patients (<65 years); second, comparison of the subgroups of patients who were using, versus not using, other sodium channel blockers (SCBs) during ESL treatment; and third, assessment of outcomes in terms of how refractory to treatment the patients were at baseline, where refractoriness was defined in terms of the number of baseline AEDs patients were receiving when ESL was initiated (less than two versus at least two concomitant AEDs).
For all subgroup analyses, effectiveness was assessed as responder and seizure freedom rates (as previously defined), safety was assessed by evaluating AEs, and tolerability was assessed by evaluating discontinuation of ESL due to AEs.
Statistical analyses
The safety population was defined as all patients who initiated ESL treatment. The efficacy population was defined as all patients who initiated ESL treatment and had at least one efficacy assessment.
There was great heterogeneity in the particular objectives of the studies included in the analysis and, thus, in the information each study reported. The current analysis attempted to combine the reported information in the most complete way possible. Missing data were not imputed, except in cross-sectional studies, in which the last visit data were captured and included in the established cut-off points (3, 6, or 12 months). When the observation timepoint of a study did not match the established cut-off points, the following allocations were made: observations performed between 1.5 and <4.5 months were allocated to the 3-month visit; those performed between 4.5 and <9 months were allocated to the 6-month visit; and those performed between 9 and 15 months were allocated to the 12-month visit. A ‘final’ variable was also created, in which the last observation of each patient was included, independently of the timepoint when it occurred. Since this was an exploratory study, no hypothesis was defined. No systematic review of the individual patients was undertaken due to the heterogeneity of the individual samples and objectives of each study. Therefore, individual studies were not treated as clusters. Similarly, adjustments at the significance level were not considered due to multiple comparisons.
A descriptive analysis of quantitative and qualitative variables was performed. For each variable, the total number of patients for whom the data in question were available was stated and this value was used as the denominator for analysis. Quantitative variables were described as mean, standard deviation (SD), median, minimum and maximum values, together with the number of valid cases and confidence intervals (CIs) or interquartile range (25th percentile to 75th percentile). Qualitative variables were described as means of absolute frequencies and percentages. Variation in the number of seizures/month between baseline and the final timepoint was assessed using the Wilcoxon signed-rank test and variation in the type of seizures was assessed using McNemar’s test. Treatment response and safety and tolerability assessments were studied as a function of the different subpopulations using the Chi-squared test or Fisher’s exact test, as appropriate. Time to ESL discontinuation was assessed using the Kaplan–Meier method. ESL dose variation between baseline and the final timepoint was assessed using the Student’s t test for repeated measures. Variation between the initial and final number of concomitant AEDs was assessed using the Wilcoxon signed-rank test. The Statistical Package for the Social Sciences version 19.0 was used for all analyses. The significance level was 5%.
Discussion
This retrospective pooled analysis of data from over 2000 European patients demonstrated that ESL was effective and generally well tolerated when used as an adjunctive treatment for partial-onset seizures under real-world clinical practice conditions over a median duration of >5 years. After 12 months of ESL treatment, 73.4% of patients were retained on ESL, 75.6% of patients had responded to treatment, and 41.3% of patients had been seizure free for ≥6 months. ESL treatment resulted in significant reductions from baseline to the last visit in the median frequencies of total, simple partial, complex partial, and secondarily generalized seizures (p < 0.01 for all). The proportions of patients with unchanged or worsened seizure frequencies remained low and relatively stable for the duration of the study (<15% and <11% of patients at all timepoints, respectively).
The most important finding of this study was that no unexpected safety signals emerged when ESL was used over the long term as adjunctive therapy for partial-onset seizures under real-world conditions. The safety profile of ESL was consistent with that observed in clinical trials [
1,
32]. The most commonly reported AEs (≥5% of patients) were dizziness (6.7%), fatigue (5.4%), and somnolence (5.1%), and the AEs leading to ESL discontinuation in ≥2% of patients were dizziness (2.3%) and fatigue (2.0%). Real-world studies complement evidence from clinical trials by providing information on how an AED performs in clinical practice, where patients are more diverse than those recruited for clinical trials and treatment is individualized on a patient-by-patient basis, rather than being administered according to a pre-defined study protocol. In addition, they allow long-term surveillance monitoring for the potential emergence of idiosyncratic AEs that are rare and/or take time to develop. The importance of long-term post-marketing surveillance is highlighted by the findings of studies conducted for other AEDs. For example, a study with felbamate reported serious side effects of aplastic anemia and hepatic toxicity [
33], and studies with retigabine (ezogabine) found idiosyncratic AEs of retinal pigment abnormalities and/or blue-grey discoloration of the lips, nail beds, hard palate, and conjunctiva emerged following long-term use [
34,
35]. This study’s findings, therefore, provide evidence indicating that ESL’s safety profile is predictable and consistent with long-term use. It is also noteworthy that the size of the patient cohort included in this study exceeds the number of patients included in ESL clinical trials (approximately 1800 patients in total [
1]). As such, the study provides robust evidence of ESL’s performance in the clinical practice setting.
Hyponatremia has been reported as a common AE in patients treated with ESL in clinical trials (1.2%) [
1], and higher rates of hyponatremia have been reported in elderly patients (8.3%) [
36]. In the current analysis, hyponatremia was reported as an AE in 3.5% of patients and led to discontinuation in 1.0% of patients. The slightly higher rate of hyponatremia reported in the clinical practice setting, in comparison with clinical trials, is perhaps unsurprising, given that the study population was more diverse in terms of age (11.7% ≥ 65 years) and comorbidities than that recruited for the randomized, controlled clinical trials. However, it is good practice to monitor for the potential development of hyponatremia with ESL treatment through laboratory testing, particularly in the elderly. Psychiatric disorders have uncommonly been reported as AEs in ESL clinical trials [
1]. In the current analysis, 3.3% of patients reported psychiatric AEs. All AEDs may have effects on mood and behavior in patients with epilepsy [
37], and, in comparison with some other AEDs, the observed incidence of psychiatric AEs in the current study was relatively low. For example, levetiracetam is commonly associated with psychiatric AEs including depression, aggression, anxiety, and irritability [
38], and in a study of 517 adult patients treated with levetiracetam, 10.1% developed psychiatric AEs [
39]. Of the patients who initiated ESL due to poor tolerability to previous AEDs, only a minority (12.0%) discontinued ESL due to AEs. The overall high retention rate observed in the study is a further indication that ESL was well tolerated in the clinical practice setting. Differences in the safety profiles of AEDs may be reflective of their particular structures and modes of action [
40,
41].
An important aspect of this study was that it included the largest population of patients treated with ESL in clinical practice to have been investigated to date, allowing meaningful subgroup analyses to be conducted. When the data were analyzed in terms of age at study entry, the effectiveness of ESL treatment was found to be significantly superior in elderly patients (≥65 years) compared with non-elderly patients (<65 years). The favorable effectiveness of ESL in elderly patients is consistent with previous findings from a multicenter, open-label, non-controlled, single-arm, Phase III trial, in which the responder and seizure freedom rates during a 26-week maintenance period were 54.9 and 15.5%, respectively [
36]. Studies of other AEDs have also demonstrated that epilepsy in elderly patients can be managed effectively, often using lower doses than those shown to be efficacious in younger patients [
42,
43]. This may be because plasma concentrations of AEDs tend to be higher in older versus younger patients, due to age-related physiological changes in, for example, protein binding, intestinal transit time, and drug elimination [
44‐
46]. Age-related changes also have an impact on AED tolerability; for example, hepatic and renal impairment affect the pharmacokinetic and pharmacodynamic properties of AEDs, increasing the likelihood of side effects, and high levels of comorbidity and polypharmacy increase the likelihood of drug–drug interactions and associated toxicity [
44,
47]. It is, therefore, perhaps unsurprising that the incidence of AEs in the current study was significantly greater in patients aged ≥65 years compared with those aged <65 years. However, it is encouraging that the rate of ESL discontinuation due to AEs was not significantly higher in those aged ≥65 versus <65 years, indicating that the AEs experienced by elderly patients were generally tolerable and/or managed effectively. A potential advantage of ESL in the geriatric setting is that it is administered once daily, reducing medication burden and increasing the likelihood of treatment adherence, in comparison with agents requiring multiple dosing per day [
48].
The subgroup analysis in which data were compared based on the number of concomitant AEDs that patients were receiving when ESL therapy was started (as a marker for treatment refractoriness) showed that, at all timepoints, responder and seizure freedom rates were significantly greater in patients treated with less than two versus at least two concomitant AEDs. These findings are consistent with those of the Eslicarbazepine acetate in Partial-Onset Seizures (EPOS) study (one of those included in the current analysis), which showed that ESL was effective as the only add-on to monotherapy in patients with refractory partial-onset seizures [
21]. The incidence of AEs and the rate of ESL discontinuation due to AEs were significantly lower in patients treated with less than two versus at least two concomitant AEDs. These results are consistent with the recognition that AEs and pharmacokinetic interactions may become more frequent as patients’ drug burden is increased, supporting recommendations to reduce levels of polytherapy wherever possible [
49,
50]. It is, therefore, encouraging that there was a significant reduction in the number of concomitant AEDs used from baseline to the last visit in the current study.
When the data in this study were analyzed in terms of the mode of action of patients’ concomitant AEDs, it was found that, at all timepoints, responder and seizure freedom rates were significantly greater in patients who were not receiving treatment with other SCBs, compared with those who were receiving treatment with other SCBs. This observation supports the idea of ‘rational polytherapy’, where AEDs with different modes of action may synergize in terms of effectiveness when used in combination [
50,
51]. It is, however, noteworthy that ESL was also effective in patients treated with other SCBs, with responder and seizure freedom rates of 70.2 and 28.6% after 12 months, respectively. These findings may reflect differences in the effects of ESL on voltage-gated sodium channels in comparison with other SCBs, since ESL selectively enhances slow inactivation of sodium channels, whereas other SCBs, such as carbamazepine and oxcarbazepine, alter the fast inactivation of sodium channels [
3]. Indeed, experimental models have demonstrated that ESL is able to overcome cellular resistance to carbamazepine [
52], which is consistent with clinical findings demonstrating that ESL can be effective in patients who have previously not achieved sufficient seizure control with carbamazepine [
53,
54].
This study was additionally able to provide insights into treatment practice when ESL is used in clinical practice. In the majority of patients, ESL was initiated due to the lack of effectiveness of previous AED therapy, although poor tolerance to previous treatment was also an important consideration for this choice of treatment. ESL was initiated at a median dose of 400 mg/day. Although the rate of titration was not analyzed, the median maintenance dose was 800 mg/day from month 3 onwards. These median initiation and maintenance doses are consistent with approved recommendations for the adjunctive treatment of adults with partial-onset seizures [
1] (and almost 90% of patients were aged 18–64 years). However, 8.9 and 0.9% of patients received an ESL dose of >1200 and >1600 mg/day, respectively, at some point during the observation period, which is above the maximum dose recommended for use as adjunctive therapy (1200 mg/day) [
1]. ESL is additionally approved as monotherapy for the treatment of partial-onset seizures, up to a maximum dose of 1600 mg/day [
1,
2]. A minority of patients in the current study (4.3%) received ESL as initial monotherapy and 17.1% of patients were receiving ESL as monotherapy at the final visit. It is currently not known whether higher doses of ESL were used in patients treated with monotherapy in the present study, as analysis of data from this patient subgroup is currently ongoing.
As a retrospective pooled analysis, this study has acknowledged limitations. As outlined previously, there was great heterogeneity in the objectives of the studies included and in the information they reported. Moreover, individual patient data were not reviewed systematically post hoc (although the data were previously reviewed by the authors of the individual studies). Nevertheless, some of the limitations of pooled analysis are likely to have been mitigated by the large number of patients included in the study population, which allowed robust statistical methodology to be employed and meaningful treatment effects to be revealed, providing long-term, real-life information on a single AED. It should also be noted that seizures were classified according to the 1981 recommendations of the International League Against Epilepsy [
30], rather than its more recent recommendations [
55], since this was the classification system originally used by the study centers involved in the clinical practice studies that comprised the analysis.
In summary, in this study, which is the largest ESL clinical practice study conducted to date, no unexpected safety signals emerged when ESL was used as adjunctive therapy for partial-onset seizures over a median duration of follow-up of >5 years. The safety profile of ESL in the clinical practice setting was found to be consistent with findings from clinical trials. ESL was shown to be an effective treatment when used under real-world conditions, with >30% of patients achieving seizure freedom at the last visit. These findings provide strong and reassuring evidence that complements data from the clinical trials, supporting the use of ESL for the treatment of partial-onset seizures.