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Journal of Assisted Reproduction and Genetics

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01.08.2010 | Gamete biology

Evaluation of Fas positive sperm and complement mediated lysis in subfertile individuals

verfasst von: Maryam Soleimani, Marziyeh Tavalaee, Roshanak Aboutorabi, Minoo Adib, Hamid Bahramian, Ehsan Janzamin, Abbas Kiani, Mohammad Hossein Nasr-Esfahani

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 8/2010

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Abstract

Purpose

Evaluation of Fas receptor on surface of sperm, as an apoptotic marker, using flow cytometry and confirming the results using an antibody-antigen complex through the classic complement pathway.

Materials and methods

Semen samples were obtained from 10 fertile and 73 infertile individuals with diagnoses of male factor infertility. Expressions of Fas receptor and phosphatidyl serine on sperm were assessed by flow cytometry. Fas expression was further assessed by antibody-antigen complex through the complement pathway. Lysis was detected via PI (propidium iodide) staining.

Results

The mean Fas expression was considerably lower than previously reported values. No significant differences in the percentage of PI were detected before and after activation of the classic complement pathway. Annexin V positive samples showed low Fas expression.

Conclusion

Our results have confirmed the presence of selected apoptotic markers such as Fas or phosphatidyl serine on ejaculate sperm, but suggest that Fas expression is low. Further studies are required to investigate the “abortive apoptosis” mechanism through Fas/Fas L.

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Titel: Evaluation of Fas positive sperm and complement mediated lysis in subfertile individuals
verfasst von: Maryam Soleimani
Marziyeh Tavalaee
Roshanak Aboutorabi
Minoo Adib
Hamid Bahramian
Ehsan Janzamin
Abbas Kiani
Mohammad Hossein Nasr-Esfahani
Publikationsdatum: 01.08.2010
Verlag: Springer US
Erschienen in: Journal of Assisted Reproduction and Genetics / Ausgabe 8/2010
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-010-9425-4

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Evaluation of Fas positive sperm and complement mediated lysis in subfertile individuals