Background
Methods
Patients
Clinicopathological variables and surgery
ICG fluorescence imaging of the resected specimens and fluorescent microscopy
Predictive factors
Statistical analysis
Results
HC group | HS group |
P
| |
---|---|---|---|
n = 116 |
n = 74 | ||
Sex (male/female) | 83/33 | 60/14 | 0.1377 |
Age (years) | 68.8 ± 9.3 | 69.6 ± 9.3 | 0.5603 |
HBV/HCV/NBC | 18/73/25 | 12/39/23 | 0.2953 |
Alcohol abuse (+) | 25 (22 %) | 14 (19 %) | 0.6613 |
Esophageal and/or gastric varices (+) | 23 (20 %) | 1 (1 %) |
0.0002
|
ICGR15 (%) | 18.8 ± 10.5 | 12.8 ± 7.6 |
<0.0001
|
Days between injection of ICG and operation | 25.5 ± 14.0 | 24.7 ± 12.3 | 0.7147 |
Platelet count (×104/mL) | 14.2 ± 6.2 | 19.6 ± 9.6 |
<0.0001
|
Total bilirubin (mg/dL) | 0.83 ± 0.33 | 0.69 ± 0.23 |
0.0016
|
Albumin (g/dL) | 3.7 ± 0.5 | 3.8 ± 0.5 | 0.5662 |
Prothrombin time (%) | 89 ± 13 | 91 ± 11 | 0.2973 |
AST (U/L) | 51 ± 27 | 37 ± 22 |
0.0002
|
ALT (U/L) | 49 ± 34 | 38 ± 33 |
0.0427
|
AFP (ng/mL) | 4103 ± 17,724 | 1829 ± 6357 | 0.3005 |
PIVKA-II (mAU/mL) | 3476 ± 11,448 | 2761 ± 9618 | 0.6657 |
HC group | HS group |
P
| |
---|---|---|---|
n = 116 |
n = 74 | ||
Operating time (min) | 349 ± 130 | 364 ± 136 | 0.423 |
Operative blood loss (mL) | 1046 ± 1031 | 1021 ± 950 | 0.8915 |
Blood transfusion (+) | 18 (16 %) | 13 (18 %) | 0.8247 |
Operative procedure | |||
Limited resection | 69 (59 %) | 22 (30 %) |
<0.0001
|
Anatomic resection | 47 (41 %) | 52 (70 %) | |
No. of patients with complications | 16 (14 %) | 8 (11 %) | 0.5462 |
Tumor size (cm) | 4.2 ± 3.5 | 5.2 ± 3.8 | 0.0798 |
Tumor differentiation | |||
Well | 30 (26 %) | 16 (22 %) | 0.4813 |
Moderate | 81 (70 %) | 52 (70 %) | |
Poor | 5 (4 %) | 6 (8 %) | |
Microvascular invasion (+) | 78 (67 %) | 53 (72 %) | 0.5246 |
Number of tumors | |||
Single | 92 (79 %) | 59 (80 %) | 0.9444 |
Multiple | 24 (21 %) | 15 (20 %) | |
Liver histology (fibrosis stage) | |||
F0 | 4 (3 %) | 12 (16 %) |
<0.0001
|
F1 | 8 (7 %) | 36 (49 %) | |
F2 | 10 (9 %) | 25 (34 %) | |
F3 | 36 (31 %) | 0 | |
F4 | 58 (50 %) | 1 (1 %) | |
Tumor stage (TMN) | |||
I or II | 58 (50 %) | 28 (38 %) | 0.1005 |
III or IV | 58 (50 %) | 46 (62 %) |
Factors affecting liver cirrhosis
Variable | Odds ratio | 95 % CI |
P
|
---|---|---|---|
Male sex | 0.47 | 0.17–1.32 | 0.1523 |
Child–Pugh class | 1.71 | 0.21–13.92 | 0.6161 |
Esophageal and/or gastric varices | 4.02 | 1.11–14.58 |
0.034
|
High cancerous in fluorescence imaging | 33.33 | 4.18–250 |
0.0009
|
ICGR15 ≥ 14 % | 3.04 | 1.20–7.66 |
0.0187
|
Albumin <3.9 g/dL | 1.11 | 0.44–2.77 | 0.8272 |
Total bilirubin ≥0.7 mg/dL | 1.63 | 0.58–4.55 | 0.3512 |
Platelet <16 × 104/mL | 3.30 | 1.24–8.77 |
0.0169
|
AST ≥39 IU/L | 1.33 | 0.52–3.38 | 0.5566 |
Classification of positive fluorescence in cancerous or non-cancerous surrounding liver tissue regions
High cancerous group | Whole type (n = 68) | Partial type (n = 48) |
P
|
Tumor differentiation | 7/59/2 |
23/22/3 |
<0.0001
|
(well/moderate/poor) | (10 %/87 %/3 %) | (48 %/46 %/6 %) | |
High surrounding group | Whole type (n = 37) | Ring type (n = 37) | |
Tumor differentiation | 8/29/0 | 8/23/6
|
0.0352
|
(well/moderate/poor) | (22 %/78 %/0) | (22 %/62 %/16 %) |