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Journal of Assisted Reproduction and Genetics
Erschienen in: Journal of Assisted Reproduction and Genetics 5/2008

01.05.2008 | Andrology

Evaluation of sperm selection procedure based on hyaluronic acid binding ability on ICSI outcome

verfasst von: M. H. Nasr-Esfahani, S. Razavi, A. A. Vahdati, F. Fathi, M. Tavalaee

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 5/2008

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Abstract

Purpose

To compare the efficiency of routine sperm selection method with HA-selection procedure for fertilization rate, embryo development, implantation and pregnancy rates as well as evaluating the relationship between HA-binding ability with sperm protamine deficiency and DNA fragmentation.

Methods

Semen samples were obtained from the 50 couples undergoing ICSI. The percentage of fertilization rate, cleavage and quality of embryos compared between two procedures (routine sperm selection and HA-binding selection). The semen samples were assessed for DNA fragmentation and protamine deficiency by sperm chromatin dispersion (SCD) test and Chromomycin A3 (CMA3) staining, respectively.

Results

A significant inverse correlation was observed between percentage of HA binding with protamine deficiency, DNA fragmentation and abnormal sperm morphology (P < 0.05). Furthermore, in current study, oocytes inseminated by HA sperm selection procedure had significantly higher fertilization rate (P < 0.05). While the pregnancy and implantation rates were insignificantly increased.

Conclusion

The results suggest that normal sperm have higher chance to bind HA and therefore, HA sperm selection procedure may select sperm with normal protamine content and low DNA fragmentation, but to confirm the effect of HA sperm selection on the ICSI outcome requires further studies.
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Metadaten
Titel
Evaluation of sperm selection procedure based on hyaluronic acid binding ability on ICSI outcome
verfasst von
M. H. Nasr-Esfahani
S. Razavi
A. A. Vahdati
F. Fathi
M. Tavalaee
Publikationsdatum
01.05.2008
Verlag
Springer US
Erschienen in
Journal of Assisted Reproduction and Genetics / Ausgabe 5/2008
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI
https://doi.org/10.1007/s10815-008-9223-4

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