Hypothermic machine preservation
Based on perfusion technique, such as single- versus double-vessel perfusion and whether active oxygenation of the perfusate is performed, hypothermic machine preservation can be categorized as follows:
-
HMP: hypothermic machine perfusion
-
HOPE: hypothermic oxygenated machine perfusion
-
D-HOPE: dual hypothermic oxygenated machine perfusion
In clinically used HMP and D-HOPE, liver grafts are perfused throughout the portal vein and the hepatic artery simultaneously, classifying them as double-vessel systems [
43‐
47]. A single vessel approach is followed in HOPE, with organ perfusion exclusively via the portal vein [
48‐
50]. While in HOPE and D-HOPE active oxygenation of the perfusate is performed [
43‐
45,
48‐
50], it is omitted in HMP [
46,
47].
The first prospective clinical trial investigating the role of HMP in human liver transplantation was reported by Guarrera et al. In 2010 [
46], a total of 20 patients receiving standard liver grafts after HMP were compared with a matched cohort of patients undergoing liver transplantation after SCS. Following the nomenclature proposal for ex vivo liver machine perfusion published by Karangwa et al., the approach used can be classified as post-static cold storage HMP (pSCS-HMP), as HMP was initiated after graft arrival in SCS at the study center, with initial SCS times exceeding 3 hours [
40]. For hypothermic dual vessel perfusion, a modified Medtronic Portable Bypass System was used, creating continuous flow through the hepatic artery and the portal vein. Although no active oxygenation of the perfusate (Vasosol®) was performed, the authors reported stable oxygen pressures throughout the perfusion (mean 137.2 ± 4.8 mmHg). Within this study, no case of PNF occurred; EAD was observed in 1 patient in the HMP group and in 5 patients in control group (
p = 0.08). One-year patient and graft survival were 90% in both groups, with no death related to graft function. However, the HMP group had significantly shorter hospital length of stay (HLS) (
p = 0.006), and significantly lower levels of post-operative peak aspartate aminotransferase (AST) (
p = 0.011), peak alanine aminotransferase (ALT) (
p = 0.044), total bilirubin (
p = 0.042), and serum creatinine (
p = 0.013) [
46]. After safety and feasibility was shown, the group expanded their inclusion criteria on investigating the role of pSCS-HMP in the transplantation of ECD grafts: in 2015, Guarrera et al. published the outcomes of 31 “orphan” ECD grafts transplanted after pSCS-HMP compared with a cohort of matched SCS liver grafts [
47]. Included organs were defined as ECD by either donor age above 65 years, hepatitis C virus positivity with 15% macrosteatosis, greater than 25% macrovesicular steatosis by biopsy, or evidence of significant donor ischemic injury (donor serum AST or ALT > 1000 IU/L) at the time of organ offer. Additionally, the term “orphan” was coined, to indicate that included grafts were either declined by all centers of their originating UNOS Region, or by all centers of the UNOS Region 9, except the study center. The study reported the occurrence of PNF in one patient of the HMP group and in two patients of the control group (
p = 0.612), with 6 cases of EAD observed in the HMP group and 9 cases in the control subjects (
p = 0.384). Three cases of vascular complications occurred in the HMP group and two cases in the SCS group, with a 1-year survival of 83.8% and 80.0%, respectively. Concerning biliary complications, significantly less cases were observed in the HMP-group (
p = 0.001). Subgroup analyses showed that especially the occurrence of biliary strictures, as a cause for a biliary complication, differed between groups (
p = 0.031). Patients in the HMP group had a significantly shorter HLS (
p = 0.001), with lower peak serum ALT on post-operative day (POD) 1 (
p = 0.049) and lower serum creatinine on POD 5 (
p = 0.02) [
47]. No adverse events due to perfusion device malfunction occurred in the reported studies [
46,
47].
HOPE was first introduced into clinical practice in 2014, as a series of 8 DCD liver transplantations published by Dutkowski et al. [
48]. The initial trial was performed as an initiative to reintroduce DCD at the University Hospital of Zürich (Switzerland), after an almost decade long hiatus of law changes. Starting with the first available DCD liver graft, a preservation protocol including HOPE was applied, with the intention to prevent adverse outcomes. Using an Organ Assist® ECOPS device, grafts were perfused solely via the portal vein with oxygenated UW gluconate solution at pressures below 3 mmHg. Six-month graft survival was 100%, with no case of PNF, delayed graft function, intrahepatic biliary cholangiopathy, or hepatic artery thrombosis, although two cases of extrahepatic biliary complications were observed [
48]. For better evaluation of the impact of HOPE in DCD liver grafts, an international-matched case analysis was published by Dutkowski et al. the following year, comparing the outcomes of
n = 25 HOPE-treated DCD liver grafts with a matched cohort of
n = 50 SCS DCD and
n = 50 SCS DBD liver transplants [
49]. Transplantation of HOPE-treated grafts was once again performed at the University Hospital of Zürich, while data of SCS DCD transplantations was obtained from the transplantation programs of Rotterdam (NL) and Birmingham (UK). Primary endpoints of the study included the incidence and severity of biliary complications within the first year after transplantation. Secondary endpoints were related to liver IRI and function and graft survival. Total cold preservation time reported was significantly shorter in the HOPE group (
p = 0.002). Comparing post-operative outcomes, HOPE-treated DCD grafts developed less cases of EAD (
p = 0.046) and showed decreased levels of peak AST (
p = 0.04), peak ALT (
p = 0.02), and peak Bilirubin (
p = 0.016) compared with SCS DCD controls. In the HOPE group, no case of PNF occurred. Regarding extrahepatic biliary complications, no differences were observed between groups, although significantly less cases of intrahepatic cholangiopathy were noted in HOPE-treated DCD grafts (
p = 0.013). Overall, 1-year graft survival was 90% in HOPE-treated, compared with 69% in SCS DCD livers (
p = 0.035) [
49]. Additionally, outcomes were compared with a matched cohort of 50 DBD SCS liver transplantations, which showed no significant differences across the analyzed endpoints [
49].
A third study applying HOPE, also performed by the Zürich group, was published in 2017: Kron et al. reported a series of 6 liver transplantations using HOPE-treated, steatotic liver grafts [
50]. This pilot trial was initiated to evaluate promising observations made in rodent experiments published within the same article. Grafts had a median macrovesicular steatosis of 30% (20–30) with 5 livers being retrieved from DCD donors. Recipient Lab-MELD score ranged from 6 to 16. [
50]. There were no cases of PNF and all patients were alive at 1-year follow-up. Compared with a cohort of DBD SCS steatotic grafts, matched for donor and recipient age as well as total preservation time, HOPE treatment showed lower ALT post reperfusion (
p = 0.04) with higher rates of 1-year patient survival (
p = 0.04) [
50].
The first study evaluating end-ischemic D-HOPE in human DCD liver transplantation was published 2017 by Van Rijn et al. from the Netherlands [
45]. The authors reported a series of 10 patients undergoing liver transplantation of DCD grafts treated with end-ischemic D-HOPE. In the trial, a Liver Assist (Organ Assist, Groningen, the Netherlands) device was used for pulsatile perfusion of the hepatic artery and creation of a continuous flow through the portal vein. Perfusate consisted of 4 l of UW Machine Perfusion Solution, supplemented with 3 mmol/l glutathione and was oxygenated at pressures above 450 mmHg, by two hollow fiber membrane oxygenators [
45]. The study reported patient and graft survivals of 100% after 6 months and 1 year. Comparing outcomes with a matched cohort of 20 SCS DCD liver transplantations, peak serum ALT levels were significantly lower in recipients of D-HOPE-treated grafts (
p = 0.006), as was serum bilirubin at POD7 (
p = 0.044). No significant differences in intensive care unit (
p = 0.475) or HLS (
p = 0.88) were observed. Of note, 3 patients of the D-HOPE group developed post reperfusion hypokalemia (
p = 0.03), without significant differences in other postoperative complications [
45].
Recently, in 2018 and 2019, two further articles reported the use of D-HOPE in liver transplantation [
43,
44]. Dondossola et al. utilized D-HOPE in 5 DCD grafts and 2 DBD grafts, which required prolonged preservation time [
44]. Patrono et al. reported the use of D-HOPE in 4 cases of higher risk DBD liver transplantation either due to donor issues, severity of liver disease in the recipient, or both [
43]. Both studies used the Liver Assist device for organ perfusion and D-HOPE was initiated after a preceding period of SCS [
43,
44]. Dondossola et al. observed 3 cases of post reperfusion syndrome (PRS) with no cases of PNF. EAD occurred in 2 cases (1 DCD and 1 DBD graft). One DBD graft included was discarded after additional viability assessment through normothermic ex vivo liver machine perfusion (NEVLP). After a median follow-up of 270 (106–582) days, patient and graft survivals were 100% with no occurrence of biliary complications [
44]. Similar outcomes were reported by Patrono et al. with patient and graft survivals of 100% at 6-month follow-up. There was no clinical evidence of ischemic cholangiopathy. In their trial, they experienced one case of post reperfusion syndrome and two cases of EAD [
43].
Normothermic ex vivo liver machine perfusion
NEVLP was first introduced into clinical practice through a Phase-I clinical trial performed by Ravikumar et al. in 2016 [
51] and shortly followed by two Northern American studies published by Selzner et al. in 2016 and Bral et al. in 2017 [
52,
53]. For organ perfusion, the portable OrganOx Metra® device was used in all studies, allowing normothermic perfusion of the portal vein and hepatic artery simultaneously [
51‐
53]. Perfusate consisted of Gelofusine® in the trials of Ravikumar et al. and Bral et al. [
51,
53], while Selzner et al. utilized Steen solution [
52]. For oxygen carriage, three units of packed red blood cells were added to the perfusate in all trials [
51‐
53]. Perfusion was initiated after organ retrieval and back table preparation at the donor center and not preceded by a period of SCS. DCD and standard DBD donors were eligible for inclusion in all studies, although the percentage of utilized DCD grafts was higher in the trial of Bral et al. (40% versus 20% in the studies of Selzner et al. and Ravikumar et al.) [
51‐
53]. The primary objective of all studies was the evaluation of the safety and the feasibility of NEVLP in human liver transplantation. In total, NEVLP of 42 grafts was reported through these trials, of which 39 were successfully transplanted [
51‐
53]. One graft was lost in the Bral et al. study due technical error (an obscure portal vein twist prevented successful NEVLP) [
53] . Two grafts reported by Selzner et al. were discarded due to poor performance during NEVLP in the context of marginal donor characteristics or anatomic unsuitability for transplantation [
52]. No case of PNF was reported throughout the trials with a 100% 30-day patient and graft survival [
51‐
53]. Follow-up was limited by a three-month interval by Selzner et al. in which no case of biliary complication or graft failure was observed [
52]. Bral et al. reported a 6-month follow-up, with no incidence of biliary complication and a patient survival of 89% [
53]. Six-month and 1-year patient survival reported by Ravikumar et al. were 100% and 95%, respectively [
51]. Intensive care unit stay and HLS of patients receiving NEVLP preserved grafts were not significantly different compared with matched SCS controls in the studies performed by Selzner et al. and Ravikumar et al. [
51,
52], while they were longer in the trial performed by Bral et al. (ICU
p = 0.004; HLS
p = 0.01) [
53]. Finally, Ravikumar et al. reported lower peak AST levels in patients undergoing transplantation of NEVLP preserved grafts compared with a SCS matched cohort (
p = 0.034) [
51].
In 2018, a landmark study in the field of NEVLP was published by Nasralla et al., reporting the first multicenter-randomized controlled trial, comparing NEVLP with SCS [
54]. In this study,
n = 334 liver grafts were randomized to either NEVLP or SCS, leading to the successful transplantation of 121 NEVLP and 101 SCS liver grafts. Seven transplant centers from four different European countries participated in the study. For graft perfusion, the OrganOx Metra® device was used. Of the included grafts, 37.1% were retrieved from DCD donors in the NEVLP arm and 36.6% in the SCS-arm [
54]. The study met its primary endpoint regarding recipient peak AST levels post transplantation, showing a reduction of peak levels by 49.4% in the NEVLP group (
p < 0.001). Subgroup analyses showed that the benefit of NEVLP, regarding peak AST, was higher for DCD grafts (
p = 0.012). Compared with respective grafts of the SCS-arm, utilization of NEVLP reduced the geometric mean peak AST levels by 73.3% in DCD grafts (
p < 0.001) and 40.2% in DBD grafts (
p = 0.001). The odds reported for developing EAD were 74% lower in the NEVLP group (12 out of 119) compared with the SCS-group (29 out of 97) (
p < 0.001), as were median serum Bilirubin levels (
p = 0.029) [
54]. No differences were reported regarding intensive care unit or HLS. Furthermore, 1-year patient and graft survival were similar, with patient survival of 95.8% vs. 97% and graft survival of 95% vs. 96% in the NEVLP and the SCS group, respectively [
54]. One case of PNF occurred in the study and was in the NEVLP group. Notably, a significantly lower amount of discard was noted in the NEVLP arm compared with the SCS arm (
p = 0.008), with longer median total preservation times in livers undergoing NEVLP (
p < 0.001) [
54].
Similar results, regarding the incidence of EAD and peak AST levels, were reported by a non-randomized phase one trial published by Liu et al. in 2020 [
55]. The trial was carried out with the purpose of evaluating fresh frozen plasma as perfusate, along with the demonstration of safety and feasibility for the use of a non-commercial, institutional developed perfusion device [
55]. NEVLP was performed in 21 liver grafts of which 38% were obtained from DCD donors. The perfusion device was carried to the retrieval site in 6 cases, while in the remainder, perfusion was started upon graft arrival at the transplant center in SCS. A maximum of 4 h cold ischemia time before NEVLP was limited by the study protocol [
55]. Using a 1:4 historical matched cohort, post-transplant outcomes demonstrated a lower incidence of EAD in the NEVLP group (19% vs. 46%;
p = 0.02) and lower levels of peak AST (
p = 0.001), peak ALT (p = 0.001), and total Bilirubin on POD7 (
p = 0.001) were reported in the NEVLP group. One-year patient survival was reported 95.23% [
55].
Relevant obstacles for introduction of NEVLP in clinic practice included logistical challenges of perfusion device transport accompanied by trained staff, as well as higher material costs compared with SCS [
4]. While hypothermic machine preservation approaches were applied end ischemic from their first introduction into clinic use [
46,
47], NEVLP original premise was to avoid preceding periods of SCS, thus requiring transport of the NEVLP device to the organ retrieval sites [
51‐
54]. Rationale for this approach was supported by a porcine study, showing inferior graft function when delaying initiation of NEVLP [
56]. In order to assess the preservation benefit of NEVLP, when accompanied by a previous period of SCS, two trials were published in 2019 comparing immediate initiation of NEVLP with a pSCS-NEVLP approach [
57,
58]: Bral et al. reported a single-center nonrandomized trial, in which 17 locally procured livers, with initiation of NEVLP immediately after graft retrieval, were compared with 26 livers retrieved from distant sites, with initiation of NEVLP after graft transportation to the study center in SCS [
57]. The principle of initiating NEVLP after graft arrival at the transplant center was coined “back to base” and performed to allow easier complementation of NEVLP. In the trial, 10 (23%) grafts were obtained from DCD donors of whom 4 were in the local NEVLP and 6 in the “back-to-base” group. SCS times in the “back to base” group were significantly longer compared with the local NEVLP subjects (
p = 0.001) with similar periods of NEVLP (
p = 0.19). Total preservation times tended to be longer in the “back-to-base group” without reaching significance (
p = 0.06) [
57]. The primary outcome, 30-day patient and graft survival, was 100% in both groups. Furthermore, no significant difference regarding patient and graft survival at 3- and 6-month (
p = 0.1), incidence of EAD (
p = 0.29), peak levels of liver function parameters in the first postoperative week (AST
p = 0.63; ALT
p = 0.95; Bilirubin
p = 0.43; INR
p = 0.95), or biliary complications (
p = 0.69) was observed [
57]. Interestingly the intensive care unit and HLS were shorter in the “back to base” group (ICU
p = 0.004; HLS
p = 0.001). Comparing the overall experience of 43 NEVLP liver transplantations reported in the trial with a matched cohort of 86 SCS grafts, the group reported similar results to those seen previously [
51,
54], observing significantly lower peak AST levels in the first post-operative week in patients undergoing transplantation after NEVLP (
p = 0.04). Furthermore, NEVLP had the logistical advantage of having more transplantations during the daytime compared with the SCS controls (
p = 0.04). [
57].
In the same year, a separate trial investigating the feasibility of pSCS-NEVLP was published by Ceresa et al. [
58]. In the multicenter study, pSCS-NEVLP was performed in 30 grafts obtained from DBD (74%) and DCD (26%) donors. This was compared with a cohort of 104 livers preserved by continuous NEVLP. The cases used for comparison were part of the NEVLP group, reported by Nasralla et al. in 2018. The study showed safety and feasibility for pSCS-NEVLP with regard to 30-day graft survival (94%) [
58]. Furthermore, no significant differences in post-transplant outcomes, concerning serum peak AST levels (
p = 0.92), incidence of EAD (
p = 0.75), post reperfusion syndrome (
p = 0.99), major complications (Clavien-Dindo ≥IIIb) (p = 0.99), and hospital (
p = 0.88) or intensive care unit (
p = 0.93) length of stay, were observed. One-year graft survival was 84% and similar to the control group (
p = 0.08) [
58]. Of note, similar to the report of Bral et al. [
57], NEVLP once again demonstrated improvements of transplantation logistics with 71% of pSCS-NEVLP liver transplantations initiated during the daytime (8 a.m. to 8 p.m.) [
58].
The clinical feasibility of the technique was further demonstrated by the Pisa (ITA) and the Innsbruck (AUT) group [
59,
60]: Ghinolfi et al. used a pSCS-NEVLP approach in a randomized single-center trial comparing NEVLP with SCS for grafts from elderly donors [
59]. In the study, 20 grafts retrieved from donors of 70 years of age or older were randomized to pSCS-NEVLP or SCS and eventually transplanted. The primary endpoint was 6-month patient and graft survival and showed non-inferiority of NEVLP preservation [
59]. Although histological findings associated NEVLP with a reduction of IRI, no significant differences in clinical outcomes were observed [
59]. Cardini et al. recently reported the introduction of routine use of pSCS-NEVLP for marginal donors, logistical challenges, and for complex recipients at the University Hospital of Innsbruck (AUT) [
60]. The authors describe a multidisciplinary approach to NEVLP and the establishment of a 24/7 applicable clinical protocol. Analyzing the first 35 cases, the authors report a major improvement in logistics through prolongation of preservation times of up to 30 h, allowing reduction of simultaneous operations and omitting nighttime transplantations. Of the first 35 cases, 25 were transplanted with a patient survival of 88% at a mean follow-up of 8.6 (± 5.9) months. Of these, 90% of the grafts were ECD [
60].
Compared with SCS and HMP approaches, NEVLP permits graft preservation in a metabolically active state, not only reducing ischemic times but also allowing for ex situ assessment of graft metabolism. Different suggestions for viability criteria have been made, although clinical evaluation is still pending [
35‐
37,
61,
62]. To date, reported viability assessments during NEVLP is based on a holistic interpretation of different perfusion parameters such as lactate clearance, bile production, perfusate pH, glucose metabolism, flow rates, and perfusate transaminases. In addition to the findings of Nasralla et al., where NEVLP lead to a lower discard rate in grafts randomized to NEVLP [
54], Cardini et al. reported that the possibility of graft evaluation via NEVLP led to increased consideration of grafts [
60]. In 2016, Mergental et al. reported successful transplantation of five liver grafts declined by all the UK centers, after viability assessment (lactate clearance, bile production, perfusate pH, hepatic artery and portal vein flows, and homogeneity of graft perfusion) via NEVLP [
36]. Patient survival was 100% after a 6–19 month follow-up, with no case of PNF reported [
36]. A further trial investigating the use of NEVLP for viability assessment in high-risk ECD grafts was published by Watson et al. (2017) [
37]. The trial reported transplantation of 12 livers assessed by NEVLP. Median donor risk index was 2.15 (1.47–3.14) with two grafts being allocated through an offer for research. Although the study reported adverse outcomes in their initial phase (observing post reperfusion syndrome in 5 of 6 grafts with one case of PNF), reevaluation of their perfusion protocol led to adjustments in oxygenation and allowed subsequent uneventful perfusions and graft evaluation which was uneventful [
37]. Specifically, changes in lactate, glucose, and transaminase concentrations, as well as maintenance of perfusate pH, were used for viability assessment and led to 1-year graft and patient survivals of 83% and 92% respectively [
37]. Most recently, Mergental et al. reported on the outcomes of the VITTAL clinical trial (
ClinicalTrials.gov NCT02740608), in which declined liver grafts were assessed by NEVLP. Included grafts must have been declared unsuitable by all the UK transplant centers in addition to meeting one of seven predefined high-risk criteria (e.g., graft macrosteatosis > 30% or peak donor transaminases > 1000 U/ml). Viability assessment was based on lactate clearance below 2.5 mmol/l within the first 4 hours of NEVLP alongside fulfillment of two or more further criteria, such as bile production, perfusate pH ≥ 7.3, metabolism of glucose, HA flow ≥ 150 ml/min and PV flow ≥ 500 ml/min, or homogenous perfusion. Of 31 assessed grafts, 22 met criteria and were eventually transplanted reaching 100% 90-day patient and graft survival [
63].