Background
Methods
Animals
Virus
Experimental Design
ELISA determination
Serum liver chemistry profile
RT-PCR detection
Indirect immunofluorescence observation
Histopathologic examination
Results
Clinical evaluation
Detection of HEV RNA by RT-nPCR
ELISA determination
Liver enzyme profile
Indirect immunofluorescence
Histopathologic examination
Group | No.1 | No.2 | No.3 | |
---|---|---|---|---|
HEV-RNA (feces) | 0d | - | - | - |
4d | - | +(4/4) | - | |
7d | - | +(4/4) | +(1/4) | |
14d | - | +(4/4) | +(1/4) | |
21d | - | - | - | |
Liver | - | +(4/4) | +(1/4) | |
Spleen | - | +(4/4) | +(1/4) | |
Kidney | - | +(4/4) | +(1/4) | |
HEV-antigen | Jejunum | - | +(4/4) | - |
Ileum | - | +(4/4) | - | |
Colon | - | +(4/4) | - | |
Anti-HEV IgG | - | +(4/4) | +(1/4) | |
IHC | - | +(4/4) | +(1/4) | |
ALT | --- | --- | --- | |
AST | --- | ↑ | ↑ | |
ALP | --- | ↑ | ↑ | |
TBIL | --- | --- | --- | |
Histologic change | no change | obvious | obvious |