Skip to main content
main-content

01.12.2019 | Research | Ausgabe 1/2019 Open Access

Diagnostic Pathology 1/2019

Expression of P63 and its correlation with prognosis in diffuse large B-cell lymphoma: a single center experience

Zeitschrift:
Diagnostic Pathology > Ausgabe 1/2019
Autoren:
Wan-Ming Hu, Jie-Tian Jin, Chen-Yan Wu, Jia-Bin Lu, Li-Hong Zhang, Jing Zeng, Su-Xia Lin
Wichtige Hinweise
Hu Wan-Ming and Jin Jie-Tian contributed equally to this work.

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Abstract

Background

Large B cell lymphoma (DLBCL) is the most common type of non-Hodgkin’s lymphoma among adults. In some cases, DLBCL may seem similar to carcinoma cells, presenting a round, oval, or polygonal shape and clear nuclei. We found that the expression of P63 accounted for a considerable proportion of DLBCL cases. Under the circumstances, P63 expression may lead to a misdiagnosis, especially with a small biopsy. We aim to investigate the expression status and prognostic significance of P63 in a cohort of Chinese DLBCL patients.

Methods

P63, ΔNP63(P40), P53 and Ki67 were detected by immunohistochemistry (IHC). A ROC curve was adopted to find the best cut-off value for positive P63/P53 expression and high Ki67 expression. We defined P53 as positive when ≥50% of the tumor cells showed staining. The relationship between P63 and P53/Ki67 expression was examined. Time-to-event endpoints were estimated according to the Kaplan-Meier method. Moreover, multivariate analyses were conducted to evaluate the prognostic factors in DLBCL.

Results

Out of all the 159 DLBCL cases, 76 (47.8%) expressed P63 in the nuclei, while 41 (25.8%) were determined to have high expression by using a ROC cut-off value “≥6”. Examination of the different P63 isoforms revealed that the ΔNP63(P40) was unclearly and weakly expressed in only 3 cases, showing a fuzzy yellow cytoplasm. P63 expression was not correlated with subtype (GCB or non-GCB) or P53 but was correlated with a high proliferative index (Ki67). Kaplan-Meier analyses revealed that P63 expression was correlated with overall survival, and P63 positive cases showed poor survival outcomes (P<0.05) in our cohort.

Conclusions

ΔNP63(P40) is a useful marker in the differential diagnosis of poorly differentiated squamous cell carcinoma versus DLBCL in small needle biopsy. P63 may be involved in DLBCL tumor progression, and it is an unfavorable prognostic marker in DLBCL. A subgroup of P63 and P53 coexpression DLBCL patients with an extremely poor prognosis should be noted.
Literatur
Über diesen Artikel

Weitere Artikel der Ausgabe 1/2019

Diagnostic Pathology 1/2019 Zur Ausgabe

Neu im Fachgebiet Pathologie

14.04.2021 | Schwerpunkt: Neue WHO-Klassifikationen und Digitales Lernen | Ausgabe 3/2021

Digitale Lehre im Fach Pathologie

06.04.2021 | Nierenkarzinom | Schwerpunkt: Neue WHO-Klassifikationen und Digitales Lernen | Ausgabe 3/2021

Histologische Subtypen des Nierenzellkarzinoms

Übersicht und neue Entwicklungen

06.04.2021 | Sarkome | Schwerpunkt: Neue WHO-Klassifikationen und Digitales Lernen | Ausgabe 3/2021

Neues in der aktuellen WHO-Klassifikation (2020) für Weichgewebssarkome