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Brain Structure and Function

Erschienen in:

01.06.2004 | Original Article

Expression of the insulin-responsive glucose transporter isoform 4 in blastocysts of C57/BL6 mice

verfasst von: S. Tonack, B. Fischer, A. Navarrete Santos

Erschienen in: Brain Structure and Function | Ausgabe 3/2004

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Abstract

Glucose is the most important energy substrate for mammalian blastocysts. In preimplantation embryos glucose uptake is mainly mediated by facilitative glucose transporter molecules (GLUT). Employing RT-PCR in 3.5-day-old mouse blastocysts of strain C57/BL6 we have investigated the expression of the GLUT isoforms 1–4 and 8. We could not only detect GLUT 1, 3 and 8 but, in contrast to earlier studies, also the insulin-responsive isoform 4. GLUT2 was not expressed. The specificity for GLUT4 amplification was verified by sequence analysis. GLUT4 protein was localized by immunohistochemistry with two GLUT4 antibodies. It was found in ICM and trophoblast cells in the cytoplasmic compartment with a strong perinuclear staining. This is the first report on the expression of the insulin-sensitive GLUT4 isoform in mouse preimplantation embryos.

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Titel: Expression of the insulin-responsive glucose transporter isoform 4 in blastocysts of C57/BL6 mice
verfasst von: S. Tonack
B. Fischer
A. Navarrete Santos
Publikationsdatum: 01.06.2004
Verlag: Springer-Verlag
Erschienen in: Brain Structure and Function / Ausgabe 3/2004
Print ISSN: 1863-2653
Elektronische ISSN: 1863-2661
DOI: https://doi.org/10.1007/s00429-004-0388-z

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