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Erschienen in:

05.09.2018 | Original Article

FGF2-responsive genes in human dental pulp cells assessed using a rat spinal cord injury model

verfasst von: Ken Sugiyama, Kosuke Nagashima, Takahiro Miwa, Yuta Shimizu, Tomoko Kawaguchi, Kazuki Iida, Naritaka Tamaoki, Daijiro Hatakeyama, Hitomi Aoki, Chikara Abe, Hironobu Morita, Takahiro Kunisada, Toshiyuki Shibata, Hidefumi Fukumitsu, Ken-ichi Tezuka

Erschienen in: Journal of Bone and Mineral Metabolism | Ausgabe 3/2019

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Abstract

The central nervous system in adult mammals does not heal spontaneously after spinal cord injury (SCI). However, SCI treatment has been improved recently following the development of cell transplantation therapy. We recently reported that fibroblast growth factor (FGF) 2-pretreated human dental pulp cells (hDPCs) can improve recovery in a rat model of SCI. This study aimed to investigate mechanisms underlying the curative effect of SCI enhanced via FGF2 pretreatment; we selected three hDPC lines upon screening for the presence of mesenchymal stem cell markers and of their functionality in a rat model of SCI, as assessed using the Basso, Beattie, and Bresnahan score of locomotor functional scale, electrophysiological tests, and morphological analyses. We identified FGF2-responsive genes via gene expression analyses in these lines. FGF2 treatment upregulated GABRB1, MMP1, and DRD2, which suggested to contribute to SCI or central the nervous system. In an expanded screening of additional lines, GABRB1 displayed rather unique and interesting behavior; two lines with the lowest sensitivity of GABRB1 to FGF2 treatment displayed an extremely minor effect in the SCI model. These findings provide insights into the role of FGF2-responsive genes, especially GABRB1, in recovery from SCI, using hDPCs treated with FGF2.

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Titel: FGF2-responsive genes in human dental pulp cells assessed using a rat spinal cord injury model
verfasst von: Ken Sugiyama
Kosuke Nagashima
Takahiro Miwa
Yuta Shimizu
Tomoko Kawaguchi
Kazuki Iida
Naritaka Tamaoki
Daijiro Hatakeyama
Hitomi Aoki
Chikara Abe
Hironobu Morita
Takahiro Kunisada
Toshiyuki Shibata
Hidefumi Fukumitsu
Ken-ichi Tezuka
Publikationsdatum: 05.09.2018
Verlag: Springer Japan
Erschienen in: Journal of Bone and Mineral Metabolism / Ausgabe 3/2019
Print ISSN: 0914-8779
Elektronische ISSN: 1435-5604
DOI: https://doi.org/10.1007/s00774-018-0954-8

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