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Journal of Assisted Reproduction and Genetics

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01.12.2014 | Assisted Reproduction Technologies

Fluctuation of “sperm DNA integrity” in accordance with semen parameters, and it’s relationship with infertility

verfasst von: Ayşen Durmaz, Nurten Dikmen, Cumhur Gündüz, Ege Nazan Tavmergen Göker, Erol Tavmergen

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 12/2014

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Abstract

Purpose

Nowadays sperm analysis is used routinely to assess infertile men, but it’s predictive value is still restricted. For this reason, new markers are needed for diagnosis and guide treatment. A DNA damage evaluation method, which is easily applicable for routine analysis is described in this paper to provide an important support in clinical evaluation.

Methods

In this study, DNA damage evaluation was planned in infertile males with the COMET assay, a method which is simple, cheap, sensitive and reliable. For this purpose, the sperm DNA damage of normospermic, oligospermic, asthenospermic and teratospermic cases were investigated and the relationship between sperm parameters (sperm count, motility, morphology) and DNA damage was assessed.

Results and conclusion

According to our results we suggest that the factors to blame for sperm DNA damage and semen quality are the same. Also it was found that the infertile males possess substantially more sperm DNA damage than fertile men do. Improving and using the DNA damage evaluation tests seem to improve the results in ART clinics.

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Titel: Fluctuation of “sperm DNA integrity” in accordance with semen parameters, and it’s relationship with infertility
verfasst von: Ayşen Durmaz
Nurten Dikmen
Cumhur Gündüz
Ege Nazan Tavmergen Göker
Erol Tavmergen
Publikationsdatum: 01.12.2014
Verlag: Springer US
Erschienen in: Journal of Assisted Reproduction and Genetics / Ausgabe 12/2014
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-014-0364-3

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Fluctuation of “sperm DNA integrity” in accordance with semen parameters, and it’s relationship with infertility

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Purpose

Methods

Results and conclusion

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Springer Medizin

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Results and conclusion

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