The online version of this article (doi:10.1186/1475-2840-11-74) contains supplementary material, which is available to authorized users.
The authors declare that they have no competing interests.
CEvdB participated in performing the study, data analysis, statistics and writing the manuscript. CSEB in part performed the study and supported the data analysis. MBK in part performed the study. SAL reviewed/edited the manuscript. CB participated in the design of the study and reviewed/edited the manuscript. RAB supervised the study, participated in the design of the study and reviewed/edited the manuscript. All authors read and approved the final manuscript.
Glucose intolerance is a major health problem and is associated with increased risk of progression to type 2 diabetes mellitus and cardiovascular disease. However, whether glucose intolerance is related to impaired myocardial perfusion is not known. The purpose of the present study was to study the effect of diet-induced glucose intolerance on myocardial function and perfusion during baseline and pharmacological induced hyperaemia.
Male Wistar rats were randomly exposed to a high fat diet (HFD) or control diet (CD) (n = 8 per group). After 4 weeks, rats underwent an oral glucose tolerance test. Subsequently, rats underwent (contrast) echocardiography to determine myocardial function and perfusion during baseline and dipyridamole-induced hyperaemia (20 mg/kg for 10 min).
Four weeks of HFD feeding resulted in glucose intolerance compared to CD-feeding. Contractile function as represented by fractional shortening was not altered in HFD-fed rats compared to CD-fed rats under baseline conditions. However, dipyridamole increased fractional shortening in CD-fed rats, but not in HFD-fed rats. Basal myocardial perfusion, as measured by estimate of perfusion, was similar in CD- and HFD-fed rats, whereas dipyridamole increased estimate of perfusion in CD-fed rats, but not in HFD-fed rats. However, flow reserve was not different between CD- and HFD-fed rats.
Diet-induced glucose intolerance is associated with impaired myocardial function during conditions of hyperaemia, but myocardial perfusion is maintained. These findings may result in new insights into the effect of glucose intolerance on myocardial function and perfusion during hyperaemia.
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- High fat diet-induced glucose intolerance impairs myocardial function, but not myocardial perfusion during hyperaemia: a pilot study
Charissa E van den Brom
Carolien SE Bulte
B Margreet Kloeze
Stephan A Loer
R Arthur Bouwman
- BioMed Central
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