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Erschienen in:
18.10.2019 | Original Article
High-fat diet with alcohol elevate oxidative stress which cause hyperlipidemia by inducing mutation in the ANGPTL3 locus
Erschienen in: International Journal of Diabetes in Developing Countries | Ausgabe 2/2020
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Background
Angiopoietin-like 3 (ANGPTL3) is a secreted protein which predominantly expressed in the liver, and involves in upregulation of lipid metabolism. Mutation in ANGPTL3 leads to decrease in the concentration of triglycerides (TGs), low-density lipoprotein (LDL), and high-density lipoprotein (HDL), which makes it a promising candidate for hyperlipidemia.
Objectives
The present study aims to identify the effect of alcohol and high-fat diet, on the ANGPTL3 locus, in the development of hyperlipidemia.
Methods
24 male Wister rats divided into four groups viz. control group (regular diet), HFD group (high-fat diet), ALC group (alcohol consuming), and HFD + ALC group (high-fat diet + alcohol consuming). All the groups were followed up for 12 weeks. Malondialdehyde (MDA), total body weight, TG, LDL, and HDL level were measured at the endpoint of study, and gene mutation analysis was also done for ANGPTL3.
Results
MDA level found to be significantly increases in HFD + ALC group, HFD group, and ALC group compared to the control group. RFLP analysis revealed a mutation in the ANGPTL3 gene which occurred only in HFD + ALC group. Moreover, body weight, TG, LDL, and HDL found to increase significantly in HFD + ALC, HFD, and ALC group as compared to the control group.
Conclusions
The present finding indicates is that high-fat diet with alcohol increases the oxidative stress in the liver, which causes a gain of function mutation in ANGPTL3 gene, resulting in hyperlipidemia.