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International Journal of Diabetes in Developing Countries

Erschienen in:

06.11.2019 | Original Article

Genetic polymorphism of eNOS (G894T) gene in insulin resistance in type 2 diabetes patients of Pakistani population

verfasst von: Abdullah Abdo Albegali, Muhammad Shahzad, Saqib Mahmood, Muhammad Ikram Ullah, Ali Amar, Osheen Sajjad

Erschienen in: International Journal of Diabetes in Developing Countries | Ausgabe 2/2020

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Abstract

Background

Insulin resistance associated with type 2 diabetes (T2DM) consequences in the development of metabolc syndrome. Due to the complex mechanism, various pathogenic factors like enviromental and genetic predispositon contribute to the disease pathogenicity. The reduced availability of eNOS has been determined as a mark of insulin resistance pathogenesis.

Objective

The objctive of this study was to investigate genetic polymorphism of eNOS (rs1799983; G894T) with susceptibility of insulin resistance in type 2 diabetes (T2DM) patients from Pakistani population.

Methods

Total of 322 (161 T2DM cases and 161 healthy controls) subjects were recruited for this study. Genomic DNA extraction was carried out by standard phenol-chloroform protocols. PCR amplification of the unique oligonucleotides of eNOS gene was done and restriction fragment analysis (RFLP) was performed by site-specific enzyme BanII.

Results

The frequency of GG (wild genotype) was higher (77.6%) in cases than in controls (47.2%), heterozygous GT genotype higher in controls than patients (OR = 0.26, p < 0.0005 and after adjustment, OR = 0.34, p = 0.091). Different genetic models like dominant model of GT&TT was higher in controls than patients (OR 0.26, p < 0.0005; OR 0.29, p = 0.038 respectively) and log-additive model indicated the significant protective effect of the genotype before and after adjustment for the confounding factors.

Conclusion

This study demonstrates that there is no association between eNOS rs1799983 polymorphism and insulin resistance in T2DM patients of Pakistani population.

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Titel: Genetic polymorphism of eNOS (G894T) gene in insulin resistance in type 2 diabetes patients of Pakistani population
verfasst von: Abdullah Abdo Albegali
Muhammad Shahzad
Saqib Mahmood
Muhammad Ikram Ullah
Ali Amar
Osheen Sajjad
Publikationsdatum: 06.11.2019
Verlag: Springer India
Erschienen in: International Journal of Diabetes in Developing Countries / Ausgabe 2/2020
Print ISSN: 0973-3930
Elektronische ISSN: 1998-3832
DOI: https://doi.org/10.1007/s13410-019-00775-6

Springer Medizin

Abstract

Background

Objective

Methods

Results

Conclusion

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