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01.08.2006 | Original Paper

Histamine Mediates the Stimulatory Action of Ghrelin on Acid Secretion in Rat Stomach

verfasst von: Koji Yakabi, Shoki Ro, Tsuneko Onouhi, Teruyuki Tanaka, Shino Ohno, Seiichi Miura, Yukimi Johno, Kiyoshige Takayama

Erschienen in: Digestive Diseases and Sciences | Ausgabe 8/2006

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Abstract

Ghrelin, a novel growth hormone-releasing peptide, is present in the rat and human stomach and is known to stimulate acid secretion and stomach motility. However, the mechanism of action of ghrelin is not fully understood. In the present study, we attempted to elucidate the role of histamine in ghrelin-induced acid secretion in rat stomach. Intravenous administration of ghrelin at 0.8 to 20 μg/kg dose dependently increased gastric acid secretion, as measured by the gastric lumen perfusion method. The maximum response was almost equal to that of gastrin (20 μg/kg). These actions were abolished by bilateral subdiaphragmatic vagotomy. Famotidine (0.33 mg/kg) also completely inhibited the effects of ghrelin. Furthermore, ghrelin increased histidine decarboxylase (HDC) messenger RNA (mRNA) levels, as measured by real-time reverse transcription-polymerase chain reaction using LightCycler. The action of ghrelin on HDC mRNA was abolished by vagotomy. Ghrelin did not affect histamine release from isolated vascularly perfused rat stomach. Taken together, these results suggest that ghrelin stimulates gastric acid secretion via a mechanism involving activation of vagal efferent nerve and histamine release from gastric enterochromaffin-like cells.

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Titel: Histamine Mediates the Stimulatory Action of Ghrelin on Acid Secretion in Rat Stomach
verfasst von: Koji Yakabi
Shoki Ro
Tsuneko Onouhi
Teruyuki Tanaka
Shino Ohno
Seiichi Miura
Yukimi Johno
Kiyoshige Takayama
Publikationsdatum: 01.08.2006
Verlag: Springer US
Erschienen in: Digestive Diseases and Sciences / Ausgabe 8/2006
Print ISSN: 0163-2116
Elektronische ISSN: 1573-2568
DOI: https://doi.org/10.1007/s10620-005-9002-3

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