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01.07.2009 | Original Article

Identification and characterization of the duck enteritis virus UL51 gene

verfasst von: Chan-Juan Shen, An-Chun Cheng, Ming-Shu Wang, Yu-Fei Guo, Li-Chan Zhao, Ming Wen, Wei Xie, Hong-Yi Xin, De-Kang Zhu

Erschienen in: Archives of Virology | Ausgabe 7/2009

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Abstract

Compared to the UL51 gene of other alphaherpesviruses, the duck enteritis virus (DEV) UL51 gene contains ten conserved motifs and has a close evolutionary relationship with members of the genus Mardivirus. The DEV UL51 gene product was identified using a rabbit polyclonal antiserum raised against a 6-His-UL51 fusion protein expressed in Escherichia coli as a 34-kDa protein. Western blotting and RT-(real time) PCR analysis of DEV-infected cells showed that the protein was produced at the late stage of infection and that its production was highly dependent on viral DNA synthesis, suggesting that the gene should be classified as γ2 class. Analysis of extracellular virions revealed that the protein was a component of extracellular mature DEV virions. Indirect immunofluorescence studies localized most of the protein to the juxtanuclear region. These results will provide a basis for further functional analysis of the gene.

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Titel: Identification and characterization of the duck enteritis virus UL51 gene
verfasst von: Chan-Juan Shen
An-Chun Cheng
Ming-Shu Wang
Yu-Fei Guo
Li-Chan Zhao
Ming Wen
Wei Xie
Hong-Yi Xin
De-Kang Zhu
Publikationsdatum: 01.07.2009
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 7/2009
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-009-0407-8

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