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Erschienen in:

01.11.2010 | Original Article

Identification of oxytetracycline as a chondrogenic compound using a cell-based screening system

verfasst von: Hironori Hojo, Fumiko Yano, Shinsuke Ohba, Kazuyo Igawa, Keiji Nakajima, Yuske Komiyama, Akinori Kan, Toshiyuki Ikeda, Takayuki Yonezawa, Je-Tae Woo, Tsuyoshi Takato, Kozo Nakamura, Hiroshi Kawaguchi, Ung-il Chung

Erschienen in: Journal of Bone and Mineral Metabolism | Ausgabe 6/2010

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Abstract

To effectively treat degenerative joint diseases including osteoarthritis (OA), small chemical compounds need to be developed that can potently induce chondrogenic differentiation without promoting terminal differentiation. For this purpose, we screened natural and synthetic compound libraries using a Col2GFP-ATDC5 system and identified oxytetracycline (Oxy) as a chondrogenic compound. Oxy induced cartilaginous matrix synthesis and mRNA expressions of chondrocyte markers in ATDC5 cells. In addition, Oxy suppressed mineralization and mRNA expressions of terminal chondrocyte differentiation markers in ATDC5 cells, primary chondrocytes, and cultured metatarsal bones. Oxy’s induction of Col2 mRNA expression was decreased by the addition of Noggin and was increased by the addition of BMP2. Furthermore, Oxy increased mRNA expression of Id1, Bmp2, Bmp4, and Bmp6. These data suggest that Oxy induces chondrogenic differentiation in a BMP-dependent manner and suppresses terminal differentiation. Oxy may be useful for treatment of OA and also for regeneration of cartilage tissue.

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Titel: Identification of oxytetracycline as a chondrogenic compound using a cell-based screening system
verfasst von: Hironori Hojo
Fumiko Yano
Shinsuke Ohba
Kazuyo Igawa
Keiji Nakajima
Yuske Komiyama
Akinori Kan
Toshiyuki Ikeda
Takayuki Yonezawa
Je-Tae Woo
Tsuyoshi Takato
Kozo Nakamura
Hiroshi Kawaguchi
Ung-il Chung
Publikationsdatum: 01.11.2010
Verlag: Springer Japan
Erschienen in: Journal of Bone and Mineral Metabolism / Ausgabe 6/2010
Print ISSN: 0914-8779
Elektronische ISSN: 1435-5604
DOI: https://doi.org/10.1007/s00774-010-0179-y

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