nach oben

Journal of Assisted Reproduction and Genetics

Erschienen in:

01.01.2009 | ANIMAL EXPERIMENTATION

Improvement of embryonic development and production of offspring by transferring meiosis-II chromosomes of senescent mouse oocytes into cytoplasts of young mouse oocytes

verfasst von: Akinori Mitsui, Midori Yoshizawa, Hiromichi Matsumoto, Emiko Fukui

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 1/2009

Einloggen, um Zugang zu erhalten

Abstract

Purpose

The effects of reciprocal transplantation of meiosis-II chromosomes between senescent and young mouse oocytes were evaluated based on pre- and post-implantation development ability of resultant embryos.

Methods

Karyoplasts including meiosis-II chromosomes of oocytes from senescent Rockefeller mouse/Ms-Rb(6, 15) females (10 to 12 months, age-related infertile mice) were transferred into cytoplasts of oocytes from young F₁ females (3 to 5 months). Reconstructed oocytes were fertilized in vitro, and then the resultant embryos were cultured in vitro and transferred to recipient mice.

Results

The reconstructed oocytes that consisted of aged-karyoplasts and young-cytoplasts showed significantly improved embryonic development (from 23.2% to 30.0%) and development to term (from 6.3% to 27.1%, P < 0.05) as compared with the oocytes reconstructed from young-karyoplasts and aged-cytoplasts.

Conclusions

The present study showed successful rejuvenation for age-related infertility using transplantation of meiosis-II chromosomes in animal experimental models.

Speroff L. The effect of aging on fertility. Curr Opin Obstet Gynecol 1994;6:115–20. doi:10.1097/00001703-199404000-00002.PubMed

Liu L, Keefe DL. Defective cohesin is associated with age-dependent misaligned chromosomes in oocytes. Reprod Biomed Online 2008;16:103–12.PubMedCrossRef

Bai ZD, Liu K, Wang XY. Developmental potential of aged oocyte rescued by nuclear transfer following parthenogenetic activation and in vitro fertilization. Mol Reprod Dev 2006;73:1448–53. doi:10.1002/mrd.20538.PubMedCrossRef

Wang MK, Chen DY, Lui JL, Li GP, Sun QY. In vitro fertilisation of mouse oocytes reconstructed by transfer of metaphase II chromosomes results in live births. Zygote 2001;9:9–14. doi:10.1017/S0967199401001022.PubMedCrossRef

Mitsui A, Yoshizawa M. Cytogenetic analysis and developmental assessment of mouse embryos derived from in vitro fertilization of oocytes reconstructed by meiosis-II chromosome transplantation. J Reprod Dev 2007;53:357–66. doi:10.1262/jrd.18114.PubMedCrossRef

Opsahl MS, Thorsell LP, Geltinger ME, Iwaszko MA, Blauer KL, Sherins RJ. Donor oocyte cytoplasmic transfer did not enhance implantation of embryos of women with poor ovarian reserve. J Assist Reprod Genet 2002;19:113–7. doi:10.1023/A:1014728603309.PubMedCrossRef

Tesarik J, Nagy ZP, Mendoza C, Greco E. Chemically and mechanically induced membrane fusion: non-activating methods for nuclear transfer in mature human oocytes. Hum Reprod 2000;15:1149–54. doi:10.1093/humrep/15.5.1149.PubMedCrossRef

Mitsui A, Yoshizawa M. Evaluation of pre-maturity of mouse oocytes ovulated from prepubertal females using an in vitro fertilization technique. J Mam Ova Res 2006;23:114–21. doi:10.1274/jmor.23.114.CrossRef

Yoshizawa M, Nakamoto S, Fukui E, Muramatsu T, Okamoto A. Chromosomal analysis of first-cleavage mouse eggs fertilized in caffeine-containing medium. J Reprod Dev 1992;38:107–13.CrossRef

10.

Yoshizawa M, Takada M, Nakamoto S, Muramatsu T, Okamoto A. Analyses of developmental ability of (BALB/c×C57BL/6) F1 and ICR mouse embryos fertilized in vitro and their chromosome at the first cleavage division. J Reprod Dev 1993;39:115–22.

11.

Barritt J, Willadsen S, Brenner C, Cohen J. Cytoplasmic transfer in assisted reproduction. Hum Reprod Update 2001;7:428–35. doi:10.1093/humupd/7.4.428.PubMedCrossRef

12.

Fair T, Murphy M, Rizos D, Moss C, Martin F, Boland MP, et al. Analysis of differential maternal mRNA expression in developmentally competent and incompetent bovine two-cell embryos. Mol Reprod Dev 2004;67:136–44. doi:10.1002/mrd.10385.PubMedCrossRef

13.

Wood JR, Dumesic DA, Abbott DH, Strauss JF 3rd. Molecular abnormalities in oocytes from women with polycystic ovary syndrome revealed by microarray analysis. J Clin Endocrinol Metab 2007;92:705–13. doi:10.1210/jc.2006–2123.PubMedCrossRef

14.

Hawes SM, Sapienza C, Latham KE. Ooplasmic donation in humans: the potential for epigenic modifications. Hum Reprod 2002;17:850–2. doi:10.1093/humrep/17.4.850.PubMedCrossRef

15.

Cohen J, Scott R, Schimmel T, Levron J, Willadsen S. Birth of infant after transfer of anucleate donor oocyte cytoplasm into recipient eggs. Lancet 1997;350:186–7. doi:10.1016/S0140-6736(05)62353-7.PubMedCrossRef

16.

Sato A, Kono T, Nakada K, Ishikawa K, Inoue S, Yonekawa H, Hayashi J. Gene therapy for progeny of mito-mice carrying pathogenic mtDNA by nuclear transplantation. Proc Natl Acad Sci USA 2005;102:16765–70. doi:10.1073/pnas.0506197102.PubMedCrossRef

17.

Ono T, Isobe K, Nakada K, Hayashi JI. Human cells are protected from mitochondrial dysfunction by complementation of DNA products in fused mitochondria. Nat Genet 2001;28:272–5. doi:10.1038/90116.PubMedCrossRef

18.

Battersby BJ, Shoubridge EA. Selection of a mtDNA sequence variant in hepatocytes of heteroplasmic mice is not due to differences in respiratory chain function or efficiency of replication. Hum Mol Genet 2001;10:2469–79. doi:10.1093/hmg/10.22.2469.PubMedCrossRef

19.

Kasahara A, Ishikawa K, Yamaoka M, Ito M, Watanabe N, Akimoto M, et al. Generation of trans-mitochondrial mice carrying homoplasmic mtDNAs with a missense mutation in a structural gene using ES cells. Hum Mol Genet 2006;15:871–8. doi:10.1093/hmg/ddl005.PubMedCrossRef

20.

Fulka H, Fulka J Jr. The use of micromanipulation methods as a tool to prevention of transmission of mutated mitochondrial DNA. Curr Top Dev Biol 2007;77:187–211. doi:10.1016/S0070-2153(06)77007-7.PubMedCrossRef

Titel: Improvement of embryonic development and production of offspring by transferring meiosis-II chromosomes of senescent mouse oocytes into cytoplasts of young mouse oocytes
verfasst von: Akinori Mitsui
Midori Yoshizawa
Hiromichi Matsumoto
Emiko Fukui
Publikationsdatum: 01.01.2009
Verlag: Springer US
Erschienen in: Journal of Assisted Reproduction and Genetics / Ausgabe 1/2009
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-008-9282-6

Update Gynäkologie

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert – ganz bequem per eMail.

Newsletter bestellen

Springer Medizin

Improvement of embryonic development and production of offspring by transferring meiosis-II chromosomes of senescent mouse oocytes into cytoplasts of young mouse oocytes

Abstract

Purpose

Methods

Results

Conclusions

Neu im Fachgebiet Gynäkologie und Geburtshilfe

Hirsutismus bei PCOS: Laser- und Lichttherapien helfen

ICI-Therapie in der Schwangerschaft wird gut toleriert

Weniger postpartale Depressionen nach Esketamin-Einmalgabe

Bei RSV-Impfung vor 60. Lebensjahr über Off-Label-Gebrauch aufklären!

Update Gynäkologie

Springer Medizin

Abstract

Purpose

Methods

Results

Conclusions

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 1/2009

Caspase-3 and VEGF immunopositivity in seminiferous tubule germ cells in cases of obstructive and non-obstructive azoospermia in smokers versus non-smokers

Apolipoprotein E gene polymorphism and polycystic ovary syndrome patients in Western Anatolia, Turkey

The correlation between male age, sperm quality and sperm DNA fragmentation in 320 men attending a fertility center

Previous tubal ectopic pregnancy raises the incidence of repeated ectopic pregnancies in In Vitro fertilization-embryo transfer patients

Comparison of letrozole with continuous gonadotropins and clomiphene-gonadotropin combination for ovulation induction in 1387 PCOS women after clomiphene citrate failure: a randomized prospective clinical trial

Intrauterine insemination with husband semen: an evaluation of pregnancy rate and factors affecting outcome

Neu im Fachgebiet Gynäkologie und Geburtshilfe

Hirsutismus bei PCOS: Laser- und Lichttherapien helfen

ICI-Therapie in der Schwangerschaft wird gut toleriert

Weniger postpartale Depressionen nach Esketamin-Einmalgabe

Bei RSV-Impfung vor 60. Lebensjahr über Off-Label-Gebrauch aufklären!

Update Gynäkologie