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12.11.2019 | Original Article

In vitro and in silico evaluations of resin-based dental restorative material toxicity

verfasst von: Susana Bandarra, Paulo Mascarenhas, Ana R. Luís, Mafalda Catrau, Evguenia Bekman, Ana C. Ribeiro, Sérgio Félix, Jorge Caldeira, Isabel Barahona

Erschienen in: Clinical Oral Investigations | Ausgabe 8/2020

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Abstract

Objectives

This study aims to evaluate the cytocompatibility of three provisional restoration materials and predict neurotoxic potential of their monomers. These materials are Tab 2000® (methyl methacrylate based), ProTemp 4™ (bis-acrylic based) and Structur 3® (urethane dimethacrylate based).

Materials and methods

Resin samples were incubated in a cell culture medium and the cytotoxic effects of these extracts were studied in 3T3 fibroblast cells through MTT and crystal violet assays as well as ROS assessment. The presence of relevant leached monomers was determined by HPLC. Additionally, the blood-brain barrier (BBB) permeability to these resin-based monomers was predicted using ACD/Labs algorithms model.

Results

Cell survival rates were compared with the resin extracts, and Structur 3® was statistically significant different from the others (p < 0.001) at all-time incubation periods. All materials induced a dose-dependent loss of cell viability; however, only Structur 3 extracts were cytotoxic against 3T3 fibroblasts. The highest cytotoxic effect (77%, p < 0.001) was observed at 24 h incubation period, which may be associated with the presence of urethane dimethacrylate (UDMA) leached monomers. Furthermore, the computational model showed that most monomers under study are expectedly capable of crossing the BBB.

Conclusions

Our results showed that Structur 3® is not cytocompatible with our cell model and UDMA is a potential neurotoxic compound.

Clinical relevance

These results indicate that only ProTemp 4™ and Tab 2000® are safe for provisional restorations.

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Titel: In vitro and in silico evaluations of resin-based dental restorative material toxicity
verfasst von: Susana Bandarra
Paulo Mascarenhas
Ana R. Luís
Mafalda Catrau
Evguenia Bekman
Ana C. Ribeiro
Sérgio Félix
Jorge Caldeira
Isabel Barahona
Publikationsdatum: 12.11.2019
Verlag: Springer Berlin Heidelberg
Erschienen in: Clinical Oral Investigations / Ausgabe 8/2020
Print ISSN: 1432-6981
Elektronische ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-019-03131-4

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In vitro and in silico evaluations of resin-based dental restorative material toxicity