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Erschienen in: Archives of Virology 12/2013

01.12.2013 | Brief Report

In vitro binding and bimolecular fluorescence complementation assays suggest an interaction between tomato mosaic virus coat protein and tobacco chloroplast ferredoxin I

verfasst von: Xianchao Sun, Yong Li, Mengdie Shi, Ning Zhang, Gaixia Wu, Tingting Li, Ling Qing, Changyong Zhou

Erschienen in: Archives of Virology | Ausgabe 12/2013

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Abstract

Symptom development of a viral plant disease results from molecular interactions between the virus and its host plant. Tomato mosaic virus coat protein (ToMV CP) not only plays a major role in virion assembly and long-distance movement but is also responsible for symptom development in ToMV-infected plants. This study provides evidence that chloroplast ferredoxin I (Fd I) interacts with ToMV CP in a GAL4-based two-hybrid yeast system for screening a Nicotiana tabacum cDNA library. The interaction between CP and Fd I was confirmed by in vitro binding and bimolecular fluorescence complementation assays in plant cells.
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Metadaten
Titel
In vitro binding and bimolecular fluorescence complementation assays suggest an interaction between tomato mosaic virus coat protein and tobacco chloroplast ferredoxin I
verfasst von
Xianchao Sun
Yong Li
Mengdie Shi
Ning Zhang
Gaixia Wu
Tingting Li
Ling Qing
Changyong Zhou
Publikationsdatum
01.12.2013
Verlag
Springer Vienna
Erschienen in
Archives of Virology / Ausgabe 12/2013
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-013-1778-4

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