Introduction
Materials and methods
Plant materials evaluated
Extraction of plant materials
Phytochemical screening
Embryonated chicken eggs for virus cultivation and antivirus assays
Hep-2 Cell lines for virus cultivation and antivirus assays
Test virus strain
Cultivation in embryonated chicken eggs
Cultivation in Hep-2 cell lines
Chicken embryo lethality assay
Spot hemagglutination
Hemagglutination assay
Screening of extracts for antiviral activity
Concentration-dependent assay of extracts for antivirus activity
Statistical analysis
Results
Plant material | Extract | pH | % yield | LD50 (mg/ml) |
---|---|---|---|---|
Leaves | LWC | 4.81 | 12.19 | 639.0 |
LHW | 5.14 | 9.15 | 616.0 | |
LET | 5.19 | 11.52 | 742.0 | |
Stems | SWC | 4.45 | 10.19 | 651.0 |
SHW | 5.12 | 7.45 | 634.0 | |
SET | 5.31 | 9.76 | 719.0 | |
Roots | RWC | 4.65 | 8.51 | 602.0 |
RHW | 5.51 | 6.45 | 626.0 | |
RET | 5.22 | 7.28 | 707.0 |
Inoculation route | Plant extract | Control | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
LWC | LHW | LET | SWC | SHW | SET | RWC | RHW | RET | PBS control | Virus control | |
Albumen | + | − | − | + | − | − | + | − | − | − | + |
Allantoic cavity | + | − | − | + | - | − | + | − | − | − | + |
Activity and morphology | Plant extract | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
LWC | LHW | LET | SWC | SHW | SET | RWC | RHW | RET | Virus control | |
Anti-MV Activity | − | + | + | − | + | + | − | + | + | − |
Hep-2 cell morphology | R | N | N | R | N | N | R | N | N | R |
Concentration (mg/ml) | % inhibition of cytopathic effect by C. cajan extracts | |||||
---|---|---|---|---|---|---|
SWC | SHW | SET | RWC | RHW | RET | |
3.91 | − | + | + | − | + | + |
7.81 | − | + | + | − | + | + |
15.63 | − | + + | + + | − | + + | + |
31.25 | − | ++ | + + | − | + + | ++ |
62.50 | − | ++ | + + | − | +++ | +++ |
125 | + | +++ | +++ | − | +++ | +++ |
250 | + | +++ | +++ | + | +++ | +++ |
Extract control | N | N | N | N | N | N |
PBS control | N | N | N | N | N | N |
Phytochemicals | Leaves | Stems | Roots | ||||||
---|---|---|---|---|---|---|---|---|---|
LWC | LHW | LET | SWC | SHW | SET | RWC | RHW | RET | |
Alkaloids | − | − | − | − | + | + | − | − | − |
Tannins | − | + | + | + | ++ | ++ | − | + | ++ |
Saponins | + | − | ++ | + | − | ++ | − | − | + |
Flavonoids | + | +++ | ++ | ++ | +++ | ++ | ++ | +++ | ++ |
Carbohydrates | + | ++ | + | + | ++ | + | + | ++ | ++ |
Glycosides | ++ | + | ++ | + | + | ++ | − | − | − |
Reducing sugars | ++ | + | ++ | + | + | ++ | + | + | ++ |