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16.09.2016 | Original Article

Increase in the nitric oxide release without changes in cell viability of macrophages after laser therapy with 660 and 808 nm lasers

verfasst von: Igor Henrique Morais Silva, Samantha Cardoso de Andrade, Andreza Barkokebas Santos de Faria, Deborah Daniela Diniz Fonsêca, Luiz Alcino Monteiro Gueiros, Alessandra Albuquerque Tavares Carvalho, Wylla Tatiana Ferreira da Silva, Raul Manhães de Castro, Jair Carneiro Leão

Erschienen in: Lasers in Medical Science | Ausgabe 9/2016

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Abstract

The aim of this study was to evaluate the influence of low-level laser therapy (LLLT) with different parameters and wavelengths on nitric oxide (NO) release and cell viability. Irradiation was performed with Ga-Al-As laser, continuous mode and wavelengths of 660 and 808 nm at different energy and power densities. For each wavelength, powers of 30, 50, and 100 mW and times of 10, 30, and 60 s were used. NO release was measured using Griess reaction, and cell viability was evaluated by mitochondrial reduction of bromide 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) to formazan. LLLT promoted statistically significant changes in NO release and MTT value only at the wavelength of 660 nm (p < 0.05). LLLT also promoted an increase in the NO release and cell viability when the energy densities 64 (p = 0.04) and 214 J/cm² (p = 0.012), respectively, were used. LLLT has a significant impact on NO release without affecting cell viability, but the significance of these findings in the inflammatory response needs to be further studied.

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Titel: Increase in the nitric oxide release without changes in cell viability of macrophages after laser therapy with 660 and 808 nm lasers
verfasst von: Igor Henrique Morais Silva
Samantha Cardoso de Andrade
Andreza Barkokebas Santos de Faria
Deborah Daniela Diniz Fonsêca
Luiz Alcino Monteiro Gueiros
Alessandra Albuquerque Tavares Carvalho
Wylla Tatiana Ferreira da Silva
Raul Manhães de Castro
Jair Carneiro Leão
Publikationsdatum: 16.09.2016
Verlag: Springer London
Erschienen in: Lasers in Medical Science / Ausgabe 9/2016
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-016-2061-1

Springer Medizin

Abstract

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