Skip to main content
Erschienen in: Lasers in Medical Science 1/2010

01.01.2010 | Original Article

Capacity of photodynamic therapy for microbial reduction in periodontal pockets

verfasst von: Sérgio Luiz Pinheiro, Juliana Marla Donegá, Lia Maura Soares Seabra, Marina Dalto Adabo, Talita Lopes, Thiago Henrique Dias do Carmo, Mariângela Cagnoni Ribeiro, Patrícia Fernanda Roesler Bertolini

Erschienen in: Lasers in Medical Science | Ausgabe 1/2010

Einloggen, um Zugang zu erhalten

Abstract

Practitioners are not successful in implemented treatments due to the great difficulty in completely removing bacterial deposits and their endotoxins. This study aimed to evaluate the capacity of photodynamic therapy to reduce the numbers of viable bacteria in periodontal pockets. Microbiological samples were collected before and after scaling and after photodynamic therapy. Photodynamic therapy was performed through the insertion of the photosensitizer toluidine blue and Endo PTC into the pocket for 3 min, followed by photosensitization with low-intensity diode of 4 J/cm2. The results (log10) were submitted to a descriptive analysis and a t-test. A reduction of 81.24% in the numbers of bacteria after scaling was observed, as well as 95.90% after photodynamic therapy (P < 0.01). Photodynamic therapy is indicated as an adjuvant treatment to reduce the numbers of viable bacteria in periodontal diseases.
Literatur
5.
Zurück zum Zitat Wilson M (2004) Lethal photosensitization of oral bacteria and its potential application in the photodynamic therapy of oral infections. Photochem Photobiol Sci 3:412–418. doi:10.1039/b211266c CrossRefPubMed Wilson M (2004) Lethal photosensitization of oral bacteria and its potential application in the photodynamic therapy of oral infections. Photochem Photobiol Sci 3:412–418. doi:10.​1039/​b211266c CrossRefPubMed
6.
Zurück zum Zitat Kömerick N, Nakanishi H, MacRobert AJ, Henderson B, Speight P, Wilson M (2003) In vivo killing of porphyromonas gingivalis by toluidine blue-mediated photosensitization in an animal model. Antimicrob Agents Chemother 47:932–940. doi:10.1128/AAC.47.3.932-940.2003 CrossRef Kömerick N, Nakanishi H, MacRobert AJ, Henderson B, Speight P, Wilson M (2003) In vivo killing of porphyromonas gingivalis by toluidine blue-mediated photosensitization in an animal model. Antimicrob Agents Chemother 47:932–940. doi:10.​1128/​AAC.​47.​3.​932-940.​2003 CrossRef
9.
Zurück zum Zitat Clark DC, Chin Quee T, Bergeron MJ, Chan ECS, Lautar-Lemay C, Gruchy K (1987) Reliability of attachment level measurements using the cementoenamel junction and a plastic stent. J Periodontol 58:115–118PubMed Clark DC, Chin Quee T, Bergeron MJ, Chan ECS, Lautar-Lemay C, Gruchy K (1987) Reliability of attachment level measurements using the cementoenamel junction and a plastic stent. J Periodontol 58:115–118PubMed
10.
Zurück zum Zitat Westergren G, Krasse B (1978) Evaluation of micromethod for determination of streptococcus mutants and lactobacillus infection. J Clin Microbiol 1:82–83 Westergren G, Krasse B (1978) Evaluation of micromethod for determination of streptococcus mutants and lactobacillus infection. J Clin Microbiol 1:82–83
12.
Zurück zum Zitat Moritz A, Gutknecht N, Doertbudak O, Goharkhay K, Schoop U, Schauer P, Sperr W (1997) Bactericidal reduction in periodontal pockets through irradiation with a diode laser: a pilot study. J Clin Laser Med Surg 15:33–37PubMed Moritz A, Gutknecht N, Doertbudak O, Goharkhay K, Schoop U, Schauer P, Sperr W (1997) Bactericidal reduction in periodontal pockets through irradiation with a diode laser: a pilot study. J Clin Laser Med Surg 15:33–37PubMed
14.
Zurück zum Zitat Jori G, Fabris C, Soncin M, Ferro S, Capellotti O, Dei D, Fantetti L, Chiti G, Roncucci G (2006) Photodynamic therapy in the treatment of microbial infections: basic principles and perspective applications. Lasers Surg Med 38:468–481. doi:10.1002/lsm.20361 CrossRefPubMed Jori G, Fabris C, Soncin M, Ferro S, Capellotti O, Dei D, Fantetti L, Chiti G, Roncucci G (2006) Photodynamic therapy in the treatment of microbial infections: basic principles and perspective applications. Lasers Surg Med 38:468–481. doi:10.​1002/​lsm.​20361 CrossRefPubMed
15.
Zurück zum Zitat Minnock A, Vernon DI, Schofield J, Griffiths J, Parish JH, Brown SB (1996) Photoinactivation of bacteria. Use of a cationic water-soluble zinc phthalocyanine to photoinactivate both Gram-negative and Gram-positive bacteria. J Photochem Photobiol B Biol 32:159–164. doi:10.1016/1011-1344(95)07148-2 CrossRef Minnock A, Vernon DI, Schofield J, Griffiths J, Parish JH, Brown SB (1996) Photoinactivation of bacteria. Use of a cationic water-soluble zinc phthalocyanine to photoinactivate both Gram-negative and Gram-positive bacteria. J Photochem Photobiol B Biol 32:159–164. doi:10.​1016/​1011-1344(95)07148-2 CrossRef
17.
Zurück zum Zitat Luan XL, Qin YL, Bi LJ, Hu CY, Zhang ZG, Lin J, Zhou CN (2009) Histological evaluation of the safety of toluidine blue-mediated photosensitization to periodontal tissues in mice. Lasers Med Sci 24:162–166. doi:10.1007/s10103-007-0513-3 Luan XL, Qin YL, Bi LJ, Hu CY, Zhang ZG, Lin J, Zhou CN (2009) Histological evaluation of the safety of toluidine blue-mediated photosensitization to periodontal tissues in mice. Lasers Med Sci 24:162–166. doi:10.​1007/​s10103-007-0513-3
18.
Zurück zum Zitat Ge LH, Shu R, Shen MH (2008) Effect of photodynamic therapy on IL-1beta and MMP-8 in gingival crevicular fluid of chronic periodontitis (in Chinese). Shanghai Kouqiang Yixue 17:10–14 Ge LH, Shu R, Shen MH (2008) Effect of photodynamic therapy on IL-1beta and MMP-8 in gingival crevicular fluid of chronic periodontitis (in Chinese). Shanghai Kouqiang Yixue 17:10–14
19.
Zurück zum Zitat Qin YL, Luan XL, Bin LJ, Sheng YQ, Zhou CN, Zhang ZG (2008) Comparison of toluidine blue-mediated photodynamic therapy and conventional scaling treatment for periodontitis in rats. J Periodontal Res 43:162–167. doi:10.1111/j.1600-0765.2007.01007.x Qin YL, Luan XL, Bin LJ, Sheng YQ, Zhou CN, Zhang ZG (2008) Comparison of toluidine blue-mediated photodynamic therapy and conventional scaling treatment for periodontitis in rats. J Periodontal Res 43:162–167. doi:10.​1111/​j.​1600-0765.​2007.​01007.​x
Metadaten
Titel
Capacity of photodynamic therapy for microbial reduction in periodontal pockets
verfasst von
Sérgio Luiz Pinheiro
Juliana Marla Donegá
Lia Maura Soares Seabra
Marina Dalto Adabo
Talita Lopes
Thiago Henrique Dias do Carmo
Mariângela Cagnoni Ribeiro
Patrícia Fernanda Roesler Bertolini
Publikationsdatum
01.01.2010
Verlag
Springer-Verlag
Erschienen in
Lasers in Medical Science / Ausgabe 1/2010
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-009-0671-6

Weitere Artikel der Ausgabe 1/2010

Lasers in Medical Science 1/2010 Zur Ausgabe