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Archives of Virology

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01.12.2011 | Original Article

Infectious bursal disease DNA vaccination conferring protection by delayed appearance and rapid clearance of invading viruses

verfasst von: Yung-Yi Chen, Ming Kun Hsieh, Chun-Yu Tung, Ching Ching Wu, Tsang Long Lin

Erschienen in: Archives of Virology | Ausgabe 12/2011

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Abstract

The present study was undertaken to determine the kinetics of viral load and immune response in protection against infectious bursal disease virus (IBDV) by DNA vaccination. Chickens were DNA-vaccinated and challenged with IBDV one week after the third vaccination. Tissues were collected at 12 hours postinfection (HPI), 1 day postinfection (DPI), 3, 5, 7 and 10 DPI. The vaccinated chickens had less viral RNA, with delayed appearance and shorter duration in the bursa of Fabricius, spleen, and cecal tonsil than the challenged control chickens. Their ELISA and neutralizing antibody titers were decreased at 12 HPI and significantly lower (P < 0.05) than those in the challenged control chickens at later time points. Their spleen IFNγ expression was up-regulated compared to that in the DNA-vaccinated chickens without IBDV challenge. These results indicate that DNA vaccination confers protection against IBDV challenge by delayed appearance and rapid clearance of the invading viruses.

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Titel: Infectious bursal disease DNA vaccination conferring protection by delayed appearance and rapid clearance of invading viruses
verfasst von: Yung-Yi Chen
Ming Kun Hsieh
Chun-Yu Tung
Ching Ching Wu
Tsang Long Lin
Publikationsdatum: 01.12.2011
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 12/2011
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-011-1127-4

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Infectious bursal disease DNA vaccination conferring protection by delayed appearance and rapid clearance of invading viruses

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