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Comparative Clinical Pathology

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07.01.2017 | Original Article

Influence of dexamethasone-induced stress on oxidative stress biomarkers in non-pregnant does experimentally infected with Brucella melitensis

verfasst von: Tanko N. Polycarp, Sabri M. Yusoff, Emikpe O. Benjamin, Shahrom M. Salisi, Siti-Khairani Bejo

Erschienen in: Comparative Clinical Pathology | Ausgabe 2/2017

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Abstract

Oxidative stress and its biomarkers are considered emerging fields in ruminant medicine. Data on this emerging field in dexamethasone-treated goats infected with Brucella melitensis is limited. This study aimed at evaluating biomarkers of oxidative stress in dexamethasone-treated non-pregnant Boer goats experimentally infected with B. melitensis. Eighteen healthy, non-pregnant goats were divided into three groups: A, B, and C of six animals each. Group A was treated with dexamethasone (2 mg/kg) for 7 days prior to inoculation with 10⁷ CFU of B. melitensis ocularly. Group B was not treated but inoculated with similar dose of B. melitensis as in group A above while group C was administered normal saline. Blood samples were collected periodically through day 42 post inoculation (pi). Plasma cortisol, malondialdehyde (MDA), xanthine oxidase (XO), superoxide dismutase (SOD), glutathione (GSH), and serological responses (independent enzyme-linked immunosorbent assay and Rose Bengal Plate Test) were analyzed. Three animals from each group were sacrificed at days 21 and 42 pi and samples from the liver, kidney, and lungs were collected and processed for further analysis. Superoxide dismutase, GSH, and XO activities increased significantly (P < 0.001) in group A during dexamethasone treatment while MDA and cortisol decreased. Following Brucella inoculation, MDA, cortisol, and XO increased significantly while GSH and SOD decreased. In group B, MDA, cortisol, and XO activities increased with less significance as compared to group A. This study demonstrated that dexamethasone-induced stress distort the redox balance significantly and for a prolonged period, consequentially leading to enhanced infectiousness of B. melitensis.

Abdollahi M, Moridani MY, Aruoma OI, Mustafalou S (2014) Oxidative stress in aging. Oxidative Med Cell Longev. doi:10.1155/2014/876834

Agarwal S, Marshall G (2001) Stress effects on immunity and its application to clinical immunology. Clin Exp Allergy 31:25–31CrossRefPubMed

Al-Garadi MA, Khairani-Bejo S, Zunita Z, Rahman AR (2011) Detection of Brucella melitensis in blood samples collected from goats. J Anim Vet Adv 10(11):1437–1444

Anderson B, Watson D, Colditz I (1999) The effect of dexamethasone on some immunological parameters in cattle. Vet Res Commun 23:399–413CrossRefPubMed

Ayala A, Muñoz MF, Argüelles S. Lipid peroxidation: production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal. Oxid Med Cell Longev. 2014;2014.

Bamaiyi PH, Hassan L, Khairani-Bejo S et al (2012) Isolation and molecular characterization of Brucella melitensis from seropositive goats in peninsula Malaysia. Trop Biomed 29:513–518PubMed

Barichello, T., Santos, A. L. B., Silvestre, C., Generoso, J. S., Cipriano, A. L., Petronilho, F., & Quevedo, J. (2011). Dexamethasone treatment reverses cognitive impairment but increases brain oxidative stress in rats submitted to pneumococcal meningitis. Oxidative medicine and cellular longevity, 2011.

Beerda B, Schilder MB, van Hooff JA, de Vries HW, Mol JA (1998) Behavioural, saliva cortisol and heart rate responses to different types of stimuli in dogs. Appl Anim Behav Sci 58(3):365–381CrossRef

Benkirane A (2006) Ovine and caprine brucellosis: world distribution and control/eradication strategies in West Asia/North Africa region. Small Rumin Res 62:19–25CrossRef

Değerlendirme U (2013) Neuroprotective effect of magnesium sulfate and dexamethasone on intrauterine ischemia in the fetal rat brain: ultrastructural evaluation. Turk Neurosurg 23:666–671

Ellman GL (1959) Tissue sulfhydryl groups. Arch Biochem Biophys 82:70–77CrossRefPubMed

Emikpe BO, Tanko PN, Onilude OM et al (2013) The influence of dexamethasone treatment and successive road transport stress on the occurrence of caprine pneumonia in a hot humid tropical environment. Vet World 6:497–501CrossRef

Enright F, Walker J, Jeffers G et al (1984) Cellular and humoral responses of Brucella abortus-infected bovine fetuses. Am J Vet Res 45:424–430PubMed

Erdogan S, Celik S, Aslantas O et al (2007) Elevated cAMP levels reverse Brucella melitensis-induced lipid peroxidation and stimulate IL-10 transcription in rats. Res Vet Sci 82:181–186CrossRefPubMed

Esterbauer H (1993) Cytotoxicity and genotoxicity of lipid-oxidation products. Am J Clin Nutr 57(5):779S–785SPubMed

Garin-Bastuji B, Blasco J. Caprine and ovine brucellosis (excluding Brucella ovis). Manual of Standards for diagnostic testes and vaccines 2008;306–308.

Gomez-Cabrera M-C, Borras C, Pallardo FV, Sastre J, Ji LL, Vina J (2005) Decreasing xanthine oxidase-mediated oxidative stress prevents useful cellular adaptations to exercise in rats. J Physiol 567:113–120

Jagtap SP, Rajak KK, Garg UK et al (2012) Effect of immunosuppression on pathogenesis of peste des petits ruminants (PPR) virus infection in goats. Microb Pathog 52:217–226CrossRefPubMed

Jenniskens FA, Jopperi-Davis KS, Walters LC, Schorr EN, Rogers LK, Welty SE, Smith CV (2002) Effects of fasting on tissue contents of coenzyme a and related intermediates in rats. Pediatr Res 52(3):437–442CrossRefPubMed

Karaagac L, Koruk ST, Koruk I, Aksoy N (2011) Decreasing oxidative stress in response to treatment in patients with brucellosis: could it be used to monitor treatment? Int J Infect Dis 15(5):e346–e349CrossRefPubMed

Kataria N, Kataria A, Maan R et al (2010) Evaluation of oxidative stress in brucella infected cows. J Stress Physiol Biochem 6:19–25

Kerksick C, Willoughby D (2005) The antioxidant role of glutathione and N-acetyl-cysteine supplements and exercise-induced oxidative stress. J Int Soc Sports Nutr 2:38–44CrossRefPubMedPubMedCentral

Khan HA, Abdelhalim MAK, Al-Ayed MS, Alhomida AS (2012) Effect of gold nanoparticles on glutathione and malondialdehyde levels in liver, lung and heart of rats. Saudi journal of biological sciences 19(4):461–464CrossRefPubMedPubMedCentral

Kohen R, Nyska A (2002) Invited review: Oxidation of biological systems: oxidative stress phenomena, antioxidants, redox reactions and methods for their quantification. Toxicol Pathol 30(6):620–650

Leal-Klevezas DS, Martinez-Vazquez IO, Garcia-Cantu J, Lopez-Merino A, Mertinez-Soriano JP (2000) Use of polymerase chain reaction to detect Brucella abortus biovar 1 in infected goats. Vet Microbiol 75(1):91–97

Lopes L, Nicolino R, Haddad J (2010) Brucellosis- risk factors and prevalence: a review. Open Vet Sci J 4:72–84CrossRef

Lu SC (1999) Regulation of hepatic glutathione synthesis: current concepts and controversies. The FASEB J 13:1169–1183PubMed

Lykkesfeldt J, Svendsen O (2007) Oxidants and antioxidants in disease: oxidative stress in farm animals. Vet J 173:502–511CrossRefPubMed

Marketon JIW, Glaser R (2008) Stress hormones and immune function. Cell Immunol 252:16–26CrossRef

Marklund S, Marklund G (1974) Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47:469–474CrossRefPubMed

Mårtensson J, Jain A, Frayer W, Meister A (1989) Glutathione metabolism in the lung: inhibition of its synthesis leads to lamellar body and mitochondrial defects. Proc Natl Acad Sci 86(14):5296–5300CrossRefPubMedPubMedCentral

Melek IM, Erdogan S, Celik S et al (2006) Evaluation of oxidative stress and inflammation in long term Brucella melitensis infection. Mol Cell Biochem 293:203–209CrossRefPubMed

Neta AVC, Mol JP, Xavier MN, Paixão TA, Lage AP, Santos RL (2010) Pathogenesis of bovine brucellosis. Vet J. 184:146–155CrossRef

Nomura J, Busso N, Ives A, et al. Xanthine oxidase inhibition by febuxostat attenuates experimental atherosclerosis in mice. Sci Rep. 2014;4.

Nwunuji P, Emikpe BO, Sabri YM (2013) Evaluation of the shedding routes and serological patterns in experimentally-induced Brucella melitensis infection in dexamethasone-treated and transport-stressed goats. Vet World 6:686–692CrossRef

Nwunuji TP, Mayowa OO, Yusoff SM, Bejo SK, Salisi S, Mohd EA (2014) The ameliorative effect of ascorbic acid on the oxidative status, live weight and recovery rate in road transport stressed goats in a hot humid tropical environment. Anim Sci J 85:611–616CrossRefPubMed

O’Leary S, Sheehan M, Sweeney T (2006) Brucella abortus detection by PCR assay in blood, milk and lymph tissue of serologically positive cows. Res Vet Sci 81(2):170–176

Ohkawa H, Ohishi N, Yagi K (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95:351–358CrossRefPubMed

Padgett DA, Glaser R (2003) How stress influences the immune response. Trends Immunol 24:444–448CrossRefPubMed

Persson T, Popescu BO, Cedazo-Minguez A. Oxidative stress in Alzheimer’s disease: why did antioxidant therapy fail? Oxid Med Cell Longev. 2014;2014.

Peterson PK, Chao CC, Molitor T et al (1991) Stress and pathogenesis of infectious disease. Rev Infect Dis 13:710–720CrossRefPubMed

Piccione G, Borruso M, Giannetto C et al (2007) Assessment of oxidative stress in dry and lactating cows. Acta Agric Scand Sect A 57:101–104

Pintea A, Zinveliu D, Pop RA, et al. Antioxidant status in dairy cows during lactation. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Veterinary Medicine 2008;63.

Polycarp, TN, Obukowho, EB, and Yusoff, SM. Changes in haematological parameters and oxidative stress response of goats subjected to road transport stress in a hot humid tropical environment. Comp Clin Path. 2015; 1–9.

Porter NA (1986) Mechanisms for the autoxidation of polyunsaturated lipids. Acc Chem Res 19(9):262–268CrossRef

Rahman I, Antonicelli F, MacNee W (1999) Molecular mechanism of the regulation of glutathione synthesis by tumor necrosis factor-α and dexamethasone in human alveolar epithelial cells. J Biol Chem 274(8):5088–5096CrossRefPubMed

Refai M (2002) Incidence and control of brucellosis in the near east region. Vet Microbiol 90:81–110CrossRefPubMed

Sahin T, Gurgoze SY, Camkerten I et al (2009) The effect of transport stress on erythrocyte glutathione peroxidase activity, plasma concentrations of lipid peroxidation and some trace element levels in beef cattle. J Anim Vet Adv 8:2223–2227

Salmon AB, Richardson A, Pérez VI (2010) Update on the oxidative stress theory of aging: does oxidative stress play a role in aging or healthy aging? Free Radical Bio Med 48:642–655CrossRef

Scandalios JG (1993) Oxygen stress and superoxide dismutases. Plant Physiol 101(1):7CrossRefPubMedPubMedCentral

Serefhanoglu K, Taskin A, Turan H et al (2009) Evaluation of oxidative status in patients with brucellosis. Braz J Infect Dis 13:249–251PubMed

Shafarin M, Zamri-Saad M, Khairani BS et al (2009) Pathological changes in the respiratory tract of goats infected by Pasteurella multocida B: 2. J Comp Pathol 140:194–197CrossRefPubMed

Swamy AV, Sajjan M, Thippeswamy AHM, Koti BC, Sadiq AJ (2011) Influence of proton pump inhibitors on dexamethasone-induced gastric mucosal damage in rats. Indian journal of pharmaceutical sciences 73(2):193CrossRefPubMedPubMedCentral

Wallwork CJ, Parks DA, Schmid-Schönbein GW (2003) Xanthine oxidase activity in the dexamethasone-induced hypertensive rat. Microvasc Res 66:30–37CrossRefPubMed

Yadav R, Mohan K, Kumar V et al (2013) Development and validation of a sensitive enzyme immunoassay (EIA) for blood plasma cortisol in female cattle, buffaloes and goats. Domest Anim Endocrinol 45:72–78CrossRefPubMed

Yin H, Xu L, Porter NA (2011) Free radical lipid peroxidation: mechanisms and analysis. Chem Rev 111(10):5944–5972CrossRefPubMed

Zamri-Saad M (2012) Detection of Brucella melitensis in seropositive goats. Online J Vet Res 16:1–7

Zamri-Saad M, Jasni S, Nurida A et al (1991) Experimental infection of dexamethasone-treated goats with Pasteurella haemolytica A2. Br Vet J 147:565–568CrossRefPubMed

Titel: Influence of dexamethasone-induced stress on oxidative stress biomarkers in non-pregnant does experimentally infected with Brucella melitensis
verfasst von: Tanko N. Polycarp
Sabri M. Yusoff
Emikpe O. Benjamin
Shahrom M. Salisi
Siti-Khairani Bejo
Publikationsdatum: 07.01.2017
Verlag: Springer London
Erschienen in: Comparative Clinical Pathology / Ausgabe 2/2017
Print ISSN: 1618-5641
Elektronische ISSN: 1618-565X
DOI: https://doi.org/10.1007/s00580-016-2395-x

Springer Medizin

Influence of dexamethasone-induced stress on oxidative stress biomarkers in non-pregnant does experimentally infected with Brucella melitensis

Abstract

Neu im Fachgebiet Pathologie

Molekularpathologische Untersuchungen im Wandel der Zeit

Vergleichende Pathologie in der onkologischen Forschung

Gastrointestinale Stromatumoren

Personalisierte Medizin in der Onkologie

Springer Medizin

Abstract

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