Introduction
Materials and methods
Study design
Ethical approval
Study participants
SNPs selection and genotyping
Statistical analyses
Results
Characteristics of participants
Variables | Control (%) | Case (%) | p-value |
---|---|---|---|
Total | 362(79.91%) | 91(20.09%) | |
PEDT | 3.17 ± 1.82 | 17.98 ± 2.79 | < 0.001 |
IELT (s) | 704.85 ± 339.07 | 70.73 ± 33.58 | < 0.001 |
Age | 41.28 ± 10.95 | 32.40 ± 6.99 | < 0.001 |
IIEF-5 score | 23.26 ± 1.33 | 22.27 ± 3.61 | < 0.001 |
5-HT (ng/mL) | 92.11 ± 97.71 | 39.47 ± 45.22 | < 0.001 |
Leptin (ng/mL) | 1.89 ± 1.40 | 1.71 ± 1.89 | 0.434 |
Folic acid (ng/mL) | 60.96 ± 57.05 | 55.38 ± 46.91 | 0.519 |
SNP | Chr | Position | Gene(s) | Role | Alleles | Frequency (MAF) |
p - HWE
| OR (95% CI) | p value | |
---|---|---|---|---|---|---|---|---|---|---|
Cases | Controls | |||||||||
rs878567 | 5 | 63,960,164 |
HTR1A
| ncRNA_intronic | G/A | 0.181 | 0.184 | 0.034 | 0.98(0.65–1.50) | 0.941 |
rs6294 | 5 | 63,961,426 |
HTR1A
| exonic | T/C | 0.181 | 0.184 | 0.034 | 0.98(0.65–1.50) | 0.941 |
rs6295 | 5 | 63,962,738 |
HTR1A
| ncRNA_intronic | G/C | 0.203 | 0.193 | 0.011 | 1.07(0.71–1.61) | 0.743 |
rs11178996 | 12 | 71,937,074 |
TPH2
| intergenic | G/A | 0.341 | 0.431 | 0.041 | 0.68(0.49–0.96) |
0.027
*
|
rs11178997 | 12 | 71,938,373 |
TPH2
| upstream | A/T | 0.231 | 0.215 | 0.212 | 1.09(0.74–1.61) | 0.655 |
rs11179001 | 12 | 71,944,865 |
TPH2
| intronic | A/G | 0.456 | 0.436 |
<0.001
| 1.08(0.78–1.50) | 0.634 |
rs10879346 | 12 | 71,958,055 |
TPH2
| intronic | T/C | 0.407 | 0.429 | 0.335 | 0.91(0.65–1.27) | 0.579 |
rs1386492 | 12 | 71,968,485 |
TPH2
| intronic | T/C | 0.434 | 0.367 | 0.572 | 1.32(0.95–1.84) | 0.098 |
rs11179023 | 12 | 71,978,617 |
TPH2
| intronic | A/G | 0.17 | 0.200 | 0.324 | 0.82(0.53–1.26) | 0.361 |
rs7305115 | 12 | 71,979,082 |
TPH2
| exonic | A/G | 0.44 | 0.384 | 0.657 | 1.26(0.91–1.75) | 0.170 |
rs11179041 | 12 | 72,010,169 |
TPH2
| intronic | A/G | 0.198 | 0.802 | 0.216 | 0.89(0.60–1.34) | 0.590 |
rs10879352 | 12 | 72,013,178 |
TPH2
| intronic | C/T | 0.198 | 0.802 | 0.218 | 0.88(0.59–1.32) | 0.548 |
rs120074175 | 12 | 72,031,544 |
TPH2
| exonic | A/G | 0.192 | 0.808 | 0.210 | 0.90(0.59–1.35) | 0.599 |
Relationship between TPH2 and HTR1A polymorphisms and LPE risk
SNP | Model | Genotype | Control | Case | OR (95% CI) | p-value |
---|---|---|---|---|---|---|
rs6295 | Co-dominant | C/C | 228(63.2%) | 59(64.8%) | 1.00 | |
C/G | 127(35.2%) | 27(29.7%) | 3.22(0.95–10.92) | 0.060 | ||
G/G | 6(1.7%) | 5(5.5%) | 0.82(0.50–1.36) | 0.445 | ||
Dominant | C/C | 228(63.2%) | 59(64.8%) | 1.00 | ||
C/G-G/G | 133(36.9%) | 32(35.2%) | 0.93(0.58–1.50) | 0.767 | ||
Recessive | C/C-C/G | 355(98.4%) | 86(94.5%) | 1.00 | ||
G/G | 6(1.7%) | 5(5.5%) | 3.44(1.03–11.54) |
0.045
*
| ||
Log-additive | --- | --- | --- | 1.08(0.70–1.65) | 0.731 | |
rs11178996 | Codominant | A/A | 127(35.1%) | 44(48.4%) | 1.00 | |
A/G | 158(43.6%) | 32(35.2%) | 0.56(0.29–1.08) | 0.083 | ||
G/G | 77(21.2%) | 15(16.5%) | 0.58(0.35–0.98) |
0.040
*
| ||
Dominant | A/A | 127(35.1%) | 44(48.4%) | 1.00 | ||
A/G-G/G | 235(64.8%) | 47(51.7%) | 0.58(0.36–0.92) |
0.020
*
| ||
Recessive | A/A-A/G | 285(78.7%) | 76(83.6%) | 1.00 | ||
G/G | 77(21.2%) | 15(16.5%) | 0.73(0.40–1.34) | 0.312 | ||
Log-additive | --- | --- | --- | 0.71(0.52–0.98) |
0.039
*
|
Relationship between TPH2 gene polymorphisms and LPE-related indicators
SNP | Model | Genotype | Leptin (ng/mL) | 5-HT (ng/mL) | Folic acid |
---|---|---|---|---|---|
rs11178997 | Co-dominant | TT(n = 29) | 2.29 ± 2.21 | 40.06 ± 50.25 | 40.06 ± 50.25 |
AT(n = 12) | 0.75 ± 0.41 | 40.30 ± 43.24 | 40.30 ± 43.24 | ||
AA(n = 7) | 0.95 ± 0.82 | 35.61 ± 28.10 | 35.61 ± 28.10 | ||
p-value |
0.027
*
| 0.972 | 0.548 | ||
Dominant | TT(n = 29) | 2.29 ± 2.21 | 40.06 ± 50.25 | 52.91 ± 44.92 | |
AT-AA(n = 19) | 0.83 ± 0.58 | 38.58 ± 37.57 | 59.16 ± 50.83 | ||
p-value |
0.007
*
| 0.913 | 0.657 | ||
Recessive | TT-AT(n = 28) | 0.95 ± 0.82 | 35.61 ± 28.10 | 73.50 ± 57.53 | |
AA(n = 7) | 1.84 ± 1.99 | 40.13 ± 47.77 | 52.29 ± 44.98 | ||
p-value | 0.255 | 0.810 | 0.274 | ||
rs10879346 | Co-dominant | TT(n = 16) | 1.25 ± 1.29 | 54.26 ± 57.45 | 45.20 ± 40.79 |
TC(n = 25) | 1.97 ± 2.26 | 27.35 ± 27.16 | 52.40 ± 40.62 | ||
CC(n = 7) | 1.85 ± 1.52 | 48.96 ± 59.33 | 89.33 ± 69.47 | ||
p-value | 0.488 | 0.149 | 0.103 | ||
Dominant | TT(n = 16) | 1.25 ± 1.29 | 54.26 ± 57.45 | 45.20 ± 40.79 | |
TC-CC(n = 32) | 1.94 ± 2.10 | 32.08 ± 36.53 | 60.47 ± 49.52 | ||
p-value | 0.232 | 0.110 | 0.293 | ||
Recessive | TT-TC(n = 41) | 1.85 ± 1.52 | 48.96 ± 59.33 | 89.33 ± 69.47 | |
CC(n = 7) | 1.69 ± 1.96 | 37.85 ± 43.09 | 49.59 ± 40.33 | ||
p-value | 0.834 | 0.554 |
0.037
*
| ||
rs1386492 | Co-dominant | CC(n = 17) | 1.78 ± 2.20 | 35.40 ± 41.60 | 68.93 ± 56.25 |
TC(n = 22) | 1.79 ± 1.79 | 27.01 ± 29.24 | 51.42 ± 44.64 | ||
TT(n = 9) | 1.40 ± 1.64 | 77.63 ± 64.89 | 39.49 ± 25.63 | ||
p-value | 0.864 |
0.013
*
| 0.277 | ||
Dominant | CC(n = 17) | 1.78 ± 2.20 | 35.40 ± 41.60 | 68.93 ± 56.25 | |
T/C-TT(n = 31) | 1.67 ± 1.73 | 41.71 ± 47.61 | 47.95 ± 40.00 | ||
p-value | 0.858 | 0.649 | 0.140 | ||
Recessive | T/C-C/C(n = 39) | 1.78 ± 1.95 | 30.67 ± 34.91 | 59.05 ± 50.11 | |
TT(n = 9) | 1.40 ± 1.64 | 77.63 ± 64.89 | 39.49 ± 25.63 | ||
p-value | 0.587 |
0.004
*
| 0.264 | ||
rs11178996 | Co-dominant | AA(n = 27) | 1.96 ± 1.44 | 43.30 ± 57.21 | 83.64 ± 66.30 |
AG(n = 13) | 2.62 ± 2.89 | 33.86 ± 34.20 | 64.40 ± 49.40 | ||
GG(n = 8) | 1.20 ± 1.14 | 41.04 ± 47.47 | 42.67 ± 34.86 | ||
p-value | 0.073 | 0.87 | 0.065 | ||
Dominant | AA(n = 27) | 1.20 ± 1.14 | 41.04 ± 47.47 | 42.67 ± 34.86 | |
AG-GG(n = 21) | 2.37 ± 2.42 | 37.46 ± 43.24 | 71.73 ± 55.63 | ||
p-value |
0.031
*
| 0.789 |
0.032
*
| ||
Recessive | AA-AG(n = 40) | 1.96 ± 1.44 | 43.30 ± 57.21 | 83.64 ± 66.30 | |
GG(n = 8) | 1.66 ± 1.97 | 38.71 ± 43.29 | 49.73 ± 40.83 | ||
p-value | 0.687 | 0.796 | 0.061 |
Relationship between haplotypes and LPE risk
Gene | SNPs | Haplotype | Freq (case) | Freq (control) | OR (95% CI) | p-value |
---|---|---|---|---|---|---|
HTR1A
| rs878567|rs6294 | GA | 0.181 | 0.184 | 0.98(0.63–1.52) | 0.938 |
AT | 0.181 | 0.184 | 0.98(0.63–1.52) | 0.938 | ||
TPH2
| rs10879346|rs1386492 | AT | 0.434 | 0.367 | 1.33(0.95–1.86) | 0.093 |
GC | 0.407 | 0.429 | 0.91(0.66–1.27) | 0.585 | ||
AC | 0.159 | 0.204 | 0.74(0.48–1.15) | 0.179 | ||
rs11179023|rs7305115 | GT | 0.440 | 0.384 | 1.28(0.91–1.79) | 0.160 | |
AC | 0.170 | 0.200 | 0.81(0.53–1.26) | 0.351 | ||
GC | 0.390 | 0.416 | 0.90(0.65–1.25) | 0.533 | ||
rs11179041|rs10879352 | AT | 0.198 | 0.209 | 0.93(0.62–1.40) | 0.737 | |
CC | 0.198 | 0.224 | 0.86(0.58–1.28) | 0.451 | ||
GT | 0.049 | 0.106 | 0.44(0.22–0.90) |
0.024
*
|