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Rheumatology International

Erschienen in:

01.03.2009 | Original Article

Intracellular calcium responses to cholinergic stimulation of lymphocytes from healthy donors and patients with rheumatoid arthritis

verfasst von: Alexander Nast, Olga Malysheva, Andreas Krause, Matthias Wahle, Christoph G. O. Baerwald

Erschienen in: Rheumatology International | Ausgabe 5/2009

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Abstract

This study was performed to identify the role of cholinergic stimulation on changes of intracellular calcium concentrations as intracellular messenger of neuroimmune interaction. Incubation of PBMC with acetylcholine (ACh) leads to Ca²⁺ oscillations in healthy controls. PBMC from rheumatoid arthritis (RA) patients exhibited increased basal Ca²⁺ concentrations with a significantly reduced capacity to respond upon ACh stimulation compared to healthy controls. It can be assumed that cholinergic signals in PBMC are mediated via the nicotinergic type of ACh receptors, causing changes in intracellular Ca²⁺ concentrations with various types of oscillations. The significantly decreased modulation of intracellular Ca²⁺ levels by ACh in PBMC of RA patients points further to a disturbed neuroimmune interaction in this chronic disease.

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Titel: Intracellular calcium responses to cholinergic stimulation of lymphocytes from healthy donors and patients with rheumatoid arthritis
verfasst von: Alexander Nast
Olga Malysheva
Andreas Krause
Matthias Wahle
Christoph G. O. Baerwald
Publikationsdatum: 01.03.2009
Verlag: Springer-Verlag
Erschienen in: Rheumatology International / Ausgabe 5/2009
Print ISSN: 0172-8172
Elektronische ISSN: 1437-160X
DOI: https://doi.org/10.1007/s00296-008-0723-4

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