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Journal of Assisted Reproduction and Genetics
Erschienen in: Journal of Assisted Reproduction and Genetics 3/2020

03.02.2020 | Genetics

Karyotype evaluation of repeated abortions in primary and secondary recurrent pregnancy loss

verfasst von: T. V. Nikitina, E. A. Sazhenova, D. I. Zhigalina, E. N. Tolmacheva, N. N. Sukhanova, I. N. Lebedev

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 3/2020

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Abstract

Purpose

To study the contribution of embryo chromosomal abnormalities in primary and secondary recurrent pregnancy loss (RPL) and to analyze the recurrence of chromosomal constitution in miscarriages from the same couple.

Methods

Retrospective study of abortion karyotypes in RPL families based on the mother’s primary or secondary RPL status (563 embryo specimens, 335 samples from primary, and 228 samples from secondary RPL). RPL was defined as two or more consecutive miscarriages. One hundred eight cases of recurrent embryo/fetal loss in 51 families were analyzed to assess the probability of having the same karyotype pattern (recurrent normal or recurrent abnormal) in both previous and subsequent pregnancy loss. The karyotypes of abortions were established using standard cytogenetic analysis, as well as interphase fluorescence in situ hybridization (FISH) and comparative genomic hybridization (CGH).

Results

The frequency of aberrations was 43.9% in abortions from primary RPL versus 52.6% in secondary RPL (p = 0.041). Women 35 years of age or older were the main contributors to this difference. The odds ratio of a subsequent abortion having the same karyotype pattern (normal or abnormal) as the previous one was 6.98 (p = 0.0013).

Conclusion

The frequency of abnormalities is higher in abortions from the secondary RPL versus primary RPL group, and this difference is due to the relative deficiency of miscarriages with abnormal karyotypes in older women with primary RPL. The probability of having the same karyotype pattern (recurrent normal or recurrent abnormal) in the previous and subsequent abortion is increased significantly compared with chance.
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Metadaten
Titel
Karyotype evaluation of repeated abortions in primary and secondary recurrent pregnancy loss
verfasst von
T. V. Nikitina
E. A. Sazhenova
D. I. Zhigalina
E. N. Tolmacheva
N. N. Sukhanova
I. N. Lebedev
Publikationsdatum
03.02.2020
Verlag
Springer US
Erschienen in
Journal of Assisted Reproduction and Genetics / Ausgabe 3/2020
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI
https://doi.org/10.1007/s10815-020-01703-y

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