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Journal of Assisted Reproduction and Genetics

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01.04.2013 | Gamete Biology

Levels of Tektin 2 and CatSper 2 in normozoospermic and oligoasthenozoospermic men and its association with motility, fertilization rate, embryo quality and pregnancy rate

verfasst von: Rashmi Bhilawadikar, Kusum Zaveri, Leena Mukadam, Shilpa Naik, Ketan Kamble, Deepak Modi, Indira Hinduja

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 4/2013

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Abstract

Purpose

To compare the expression profiles of Tektin 2 and CatSper 2 motility proteins in the spermatozoa of normozoospermic and oligoasthenozoospermic men and determine its correlation with sperm motility, fertilization rate, embryo quality and pregnancy rate.

Methods

Tektin 2 and CatSper 2 protein expression was studied using Western Blotting and immunofluorescence. Tektin 2 and CatSper 2 protein levels were quantified by ELISA.

Results

Oligoasthenozoospermic men were found to have lower fertilization rates, poor embryo quality and lower pregnancy rates as compared to normozoospermic men. The levels of Tektin 2 and CatSper 2 are significantly lower in spermatozoa of oligoasthenozoospermic men as compared to normozoospermic controls; the levels were also lower in immotile fraction as compared to motile fraction of spermatozoa obtained from normozoospermic individuals. The levels of Tektin 2 and CatSper 2 were higher in individuals demonstrating sperm motility >60 % as compared to sperm motility <30 %. Tektin 2 but not CatSper 2 levels were positively associated with fertilization rate, embryo quality and pregnancy rate.

Conclusion

Levels of Tektin 2 and CatSper 2 proteins are positively associated with sperm motility parameters. Measurements of Tektin 2 levels can be correlated with the clinical outcome of ICSI.

Abid S, Gokral J, Maitra A, Meherji P, Kadam S, Pires E, et al. Altered expression of progesterone receptors in testis of infertile men. RBM Online. 2008;17:175–84.PubMed

Avenarius MR, Hildebrand MS, Zhang Y, Meyer NC, Smith Luke LH, Kahrizi K, et al. Human male infertility caused by mutations in the Catsper1 Channel Protein. Am J Hum Genet. 2009;84:505–10.PubMedCrossRef

Avidan N, Tamary H, Dgany O, Cattan D, Pariente AE, Thulliez M, et al. CATSPER2, a human autosomal nonsyndromic male infertility gene. Eur J Hum Genet. 2003;11:497–502.PubMedCrossRef

Blerkom JV, Davis P, Merriam J, Sinclair J. Nuclear and cytoplasmic dynamics of sperm penetration, pronuclear formation and microtubule organization during fertilization and early preimplantation development in the human. Hum Reprod Updat. 1995;1:429–61.CrossRef

Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72:248–54.PubMedCrossRef

Cai ZM, Gui YT, Guo X, Yu J, Guo LD, Zhang LB, et al. Low expression of glycoprotein subunit 130 in ejaculated spermatozoa from asthenozoospermic men. J Androl. 2006;27:645–52.PubMedCrossRef

Carlson AE, Westenbroek RE, Quill T, Ren D, Clapham DE, Hille B, et al. CatSper 1 required for evoked Ca²⁺ entry and control of flagellar function in sperm. Proc Natl Acad Sci. 2003;100:14864–8.PubMedCrossRef

Chan CC, Shui HA, Wu CH, Wang CY, Sun GH, Chen HM, et al. Motility and protein phosphorylation in healthy and asthenozoospermic sperm. J Proteome Res. 2009;8:5382–6.PubMedCrossRef

Chang XJ, Piperno G. Cross reactivity of antibodies specific for flagellar Tektin and intermediate filament subunits. J Cell Biol. 1987;104:1563–8.PubMedCrossRef

10.

Chetrit AB, Senoz S, Greenblatt EM, Casper RF. In vitro fertilization outcome in the presence of severe male factor infertility. Fertil Steril. 1995;63:1032–7.

11.

Heredia JM, de Mateo S, Vidal-Taboada JM, Ballescà JL, Oliva R. Identification of proteomic differences in asthenozoospermic sperm samples. Hum Reprod. 2008;23:783–91.CrossRef

12.

Hinduja IN, Mehta RH, Gopalkrishnan K, Anandkumar TC. Manual for human in vitro fertilization – Embryo transfer and Gamete Intra-Fallopian Transfer, 1st ed. ICMR; 1991 pp 24–28.

13.

Hinduja I, Zaveri K, Baliga N. Human sperm centrin levels & outcome of intracytoplasmic sperm injection (ICSI) - A pilot study. Indian J Med Res. 2008;128:606–10.PubMed

14.

Hoshi K, Katayose H, Yanagida K, Sato A, Yazawa H. Intra cytoplasmic sperm injection using immobilizes or motile human spermatozoon. Fertil Steril. 1995;63:1241–5.PubMed

15.

Iguchi N, Tanaka H, Nakamura Y, Nozaki M, Fujiwara T, Nishimune Y. Cloning and characterization of the human tektin-t gene. Mol Hum Reprod. 2002;8:525–30.PubMedCrossRef

16.

Jin JL, O’doherty AM, Wang S, Zheng H, Sanders KM, Yan W. Catsper 3 and Catsper 4 encode two cation channel like proteins exclusively expressed in the testis. Biol Reprod. 2005;73:1235–42.PubMedCrossRef

17.

Kahraman S, Tasdemir M, Polat I, Islk AZ, Biberoglu K, Vanderzwalmen P, et al. Pregnancies achieved with testicular and ejaculated spermatozoa in combination with intracytoplasmic sperm injection in men with totally or initially immotile spermatozoa in the ejaculate. Hum Reprod. 1996;11:1343–6.PubMedCrossRef

18.

Kovalski N, Lamirande R, Gagnon C. Reactive oxygen species generated by human neutrophils inhibit sperm motility, protective effect of seminal plasma and scavengers. Fertil Steril. 1992;58:809–16.PubMed

19.

Kruger TF, Menkveld R, Stander FSH, Lombard CJ, Van der Merwe JP, Zyl JA, et al. Sperm morphologic factors as a prognostic factor in in-vitro fertilization. Fertil Steril. 1986;46:118–1123.

20.

Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970;227:680–5.PubMedCrossRef

21.

Lambard S, Galeraud-Denis I, Bouraima H, Bourguiba S, Chocat A, Carreau S. Expression of aromatase in human ejaculated spermatozoa: a putative marker of motility. Mol Hum Reprod. 2003;9:117–24.PubMedCrossRef

22.

Li H-G, Ding X-F, Liao A-H, Kong X-B, Xiong C-L. Expression of CatSper family transcripts in the mouse testis during post-natal development and human ejaculated spermatozoa: relationship to sperm motility. Mol Hum Reprod. 2007;13:299–306.PubMedCrossRef

23.

Li H-G, Ding X-F, Liao A-H, Zhou H, Xiong C-L. The expression and significance of Catsper1 in human testis and ejaculated spermatozoa. Asian J Androl. 2006;8:301–6.PubMedCrossRef

24.

Lipshultz L. Subfertility. In: Kaufman J, editor. Current urology therapy. Philadelphia: WB Saunders; 1980.

25.

Liu J, Nagy Z, Joris H, Tournaye H, Smitz J, Camus M, et al. Analysis of 76 total fertilization failure cycles out 2732 ICSI cycle. Hum Reprod. 1995;10:2630–6.PubMed

26.

Nagvenkar P, Zaveri K, Hinduja I. Comparison of the sperm aneuploidy rate in severe oligozoospermic and oligozoospermic men and its relation to intracytoplasmic sperm injection outcome. Fertil Steril. 2005;8:925–31.CrossRef

27.

Nagy ZP, Liu J, Joris H, Verheyen G, Tournaye H, Camus M, et al. The result of intracytoplasmic sperm injection is not related to any of the three basic sperm parameters. Hum Reprod. 1995;10:1123–9.PubMed

28.

Nijs M, Vanderzwalmen P, Vandamme B, G-Bertin S, Lejeune B, Segal L, et al. Fertilizing ability of immotile spermatozoa after intracytoplasmic sperm injection. Hum Reprod. 1996;11:2180–5.PubMedCrossRef

29.

Nikpoor P, Mowla SJ, Movahedin M, Ziaee SA-M, Tiraihi T. Catsper gene expression in postnatal development of mouse testis and in subfertile men with deficient sperm motility. Hum Reprod. 2004;19:124–8.PubMedCrossRef

30.

Overstreet JW, Gould JE, Katz DF, Hanson FW. In vitro capacitation of human spermatozoa after passage through a column of cervical mucus. Fertil Steril. 1980;34:604–6.PubMed

31.

Palermo GD, Adler A, Cohen J, Rosenwake Z, Alikani M. Intracytoplasmic sperm injection: a novel treatment for all forms of male factor infertility. Fertil Steril. 1995;63:1231–40.PubMed

32.

Pirner MA, Linck RW. Tektins are heterodimeric polymers in flagellar microtubules with axial periodicities matching the tubulin lattice. J Biol Chem. 1994;269:31800–6.

33.

Quill T, Ren D, Clapham DE, Garbers DL. A voltage-gated ion channel expressed specifically in spermatozoa. Proc Natl Acad Sci. 2001;98:12527–31.PubMedCrossRef

34.

Quill TA, Sugden SA, Rossi KL, Doolittle LK, Hammer RE, Garbers DL. Hyperactivated sperm motility driven by CatSper 2 is required for fertilization. Proc Natl Acad Sci U S A. 2003;100:14869–74.

35.

Ren D, Navarro B, Perez G, Jackson AC, Hsu S, Shi Q, et al. A sperm ion channel required for sperm motility and male fertility. Nature. 2001;413:603–9.PubMedCrossRef

36.

Roy A, Lin YN, Agno JE, DeMayo FJ, Matzuk MM. Absence of tektin 4 causes asthenozoospermia and subfertility in male mice. FASEB. 2007;21:1013–25.CrossRef

37.

Shah C, Modi D, Sachdeva G, Gadkar S, D’Souza S, Puri C. N-terminal region of progesterone receptor B isoform in human spermatozoa. Int J Androl. 2005;28:360–71.PubMedCrossRef

38.

Siva AB, Kameshwari DB, Singh V, Pavani K, Sundaram CS, Rangaraj N, et al. Proteomics-based study on asthenozoospermia: differential ex-pression of proteasome alpha complex. Mol Hum Reprod. 2010;16:452–62.PubMedCrossRef

39.

Steffen W, Linck RW. Evidence for tektins in centrioles and axonemal microtubules. Proc Natl Acad Sci. 1988;85:2643–7.PubMedCrossRef

40.

Takuya M, Yoshiko H, Masamichi D, Hiroshi I. Molecular Cloning of a now member of TEKTIN family, Tektin 4, located to the flagella of rat spermatozoa. Mol Reprod Dev. 2005;72:120–8.CrossRef

41.

Tanaka H, Iguchi N, Toyama Y, Kitamura K, Takahashi T, Kaseda K, et al. Mice deficient in the axonemal protein tektin-t exhibit male infertility and immotile-cilium syndrome due to impaired inner arm dynein function. Mol Cell Biol. 2004;24:7958–64.PubMedCrossRef

42.

Turhan N, Pekel A, Ayrim A, Bayrak O. ICSI outcome in severely oligoasthenozoospermic patients and its relationship to prewash progressive sperm motility. Turk J Med Sci. 2011;4:995–9.

43.

Turner RM. Moving to the beat: a review of mammalian sperm motility regulation. Reprod Fertil Dev. 2006;18:25–38.PubMedCrossRef

44.

Veeck LL. Pre embryo grading. Atlas of Human Oocytes and early conceptus, Vol 2. USA: Williams and Wilkins; 1991. p. 121–44.

45.

Vendrell JM, Arán B, Veiga A, García F, Coroleu B, Egozcue S, et al. Spermatogenic patterns and early embryo development after intracytoplasmic sperm injection in severeoligoasthenozoospermia. J Assist Reprod Genet. 2003;20:106–12.PubMedCrossRef

46.

Verheyen G, Tournaye H, Staessen C, De Vos A, Vandervorst M, Steirteghem AV. Controlled comparison of conventional in-vitro fertilization and intracytoplasmic sperm injection in patients with asthenozoospermia. Hum Reprod. 1999;14:2313–9.PubMedCrossRef

47.

WHO Laboratory manual for the examination of Human Semen and sperm-cervical mucus Interaction, World Health Organization, 4th ed. Cambridge University Press; 1999 pp 6–33 and 104–105.

48.

Xia P. Intra cytoplasmic sperm injection: correlation of oocyte grade based on polar body, perivitelline space and cytoplasmic inclusions with fertilization rate and embryo quality. Hum Reprod. 1997;12:1750–5.PubMedCrossRef

49.

Zhao C, Huo R, Wang FQ, Lin M, Zhou ZM, Sha JH. Identification of several proteins involved in regulation of sperm motility by proteomic analysis. Fertil Steril. 2007;87:436–8.PubMedCrossRef

50.

Zuccarello D, Ferlin A, Cazzadore C, Pepe A, Garolla A, Moretti A, et al. Mutations in dynein genes in patients affected by isolated non-syndromic asthenozoospermia. Hum Reprod. 2008;23:1957–62.PubMedCrossRef

Titel: Levels of Tektin 2 and CatSper 2 in normozoospermic and oligoasthenozoospermic men and its association with motility, fertilization rate, embryo quality and pregnancy rate
verfasst von: Rashmi Bhilawadikar
Kusum Zaveri
Leena Mukadam
Shilpa Naik
Ketan Kamble
Deepak Modi
Indira Hinduja
Publikationsdatum: 01.04.2013
Verlag: Springer US
Erschienen in: Journal of Assisted Reproduction and Genetics / Ausgabe 4/2013
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-013-9972-6

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Levels of Tektin 2 and CatSper 2 in normozoospermic and oligoasthenozoospermic men and its association with motility, fertilization rate, embryo quality and pregnancy rate