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Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie

Erschienen in:

22.03.2022 | Original Article

Ablation of FAM20C caused short root defects via suppressing the BMP signaling pathway in mice

verfasst von: Lili Li, Peihong Liu, Xuechao Lv, Tianliang Yu, Xingai Jin, Rui Wang, Xiaohua Xie, Qingshan Wang, Yingqun Liu, Wuliji Saiyin

Erschienen in: Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie | Ausgabe 6/2023

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Abstract

Short root defects are prone to cause various periodontal diseases and lead to tooth loss in some serious cases. Studies about the mechanisms governing the development of the root are needed for a better understanding of the pathogenesis of short root defects. The protein family with sequence similarity 20 group C (FAM20C) is a Golgi casein kinase that has been well studied in the development of tooth crown formation. However, whether FAM20C plays a role in the development of tooth root is still unknown. Thus, we generated Sox2-Cre;Fam20c^fl/fl (cKO) mice, in which Fam20c was ablated in both the dental epithelium and dental mesenchyme, and found that the cKO mice showed severe short root defects mainly by inhibiting the development of dental mesenchyme in the root region. In this investigation, we found morphological changes and differentiation defects, with reduced expression of dentin sialophosphoprotein (DSPP) in odontoblasts of the root region in cKO mice. Furthermore, the proliferation rate of apical papillary cells was reduced in the root of cKO mice. In addition, the levels of bone morphogenetic protein 4 (BMP4) and phospho-Smad1/5/8, and that of Osterix and Krüppel-like factor 4 (KLF4), two downstream target molecules of the BMP signaling pathway, were significantly reduced in the root of cKO mice. These results indicate that FAM20C plays an essential role in the development of the root by regulating the BMP signaling pathway.

Sozzani R, Cui H, Moreno-Risueno MA, Benfey PN et al (2010) Spatiotemporal regulation of cell-cycle genes by SHORTROOT links patterning and growth. Nature 466(7302):128–132. https://doi.org/10.1038/nature09143CrossRefPubMedPubMedCentral

Chen H, Guo S, Xia Y, Ma J et al (2018) The role of Rho-GEF trio in regulating tooth root development through the p38 MAPK pathway. exp Cell Res 372(2):158–167. https://doi.org/10.1016/j.yexcr.2018.09.022CrossRefPubMed

Huang H, Wang J, Zhang Y, Chen W et al (2018) Bone resorption deficiency affects tooth root development in RANKL mutant mice due to attenuated IGF‑1 signaling in radicular odontoblasts. Bone 114(09):161–171. https://doi.org/10.1016/j.bone.2017.12.026CrossRefPubMed

Romero FJM, Star H, Lav R, Tucker AS et al (2017) The impact of the Eda pathway on tooth root development. J Dent Res 96(11):1290–1297. https://doi.org/10.1177/0022034517725692CrossRef

Liu Y, Feng J, Li J, Chai Y et al (2015) An Nfic-hedgehog signaling cascade regulates tooth root development. Development 142(19):3374–3382. https://doi.org/10.1242/dev.127068CrossRefPubMedPubMedCentral

Moulton MJ, Humphreys GB, Kim A, Letsou A et al (2020) O‑GlcNAcylation dampens Dpp/BMP signaling to ensure proper drosophila embryonic development. Dev Cell 53(3):330–343.e3. https://doi.org/10.1016/j.devcel.2020.04.001CrossRefPubMed

Jin SL, Bai YM, Zhao BY, Zhang HS et al (2020) Silencing of miR-330-5p stimulates osteogenesis in bone marrow mesenchymal stem cells and inhibits bone loss in osteoporosis by activating Bgn-mediated BMP/Smad pathway. Eur Rev Med Pharmacol Sci 24(8):4095–4102. https://doi.org/10.26355/eurrev_202004_20987CrossRefPubMed

Liu CW, Zhou YJ, Yan GX, Sun HC et al (2018) The role of bone morphogenetic protein signaling pathway in tooth root development. Hua Xi Kou Qiang Yi Xue Za Zhi 36(5):559–563. https://doi.org/10.7518/hxkq.2018.05.017CrossRefPubMed

Rakian A, Yang WC, Gluhak-Heinrich J, Harris SE et al (2013) Bone morphogenetic protein‑2 gene controls tooth root development in coordination with formation of the periodontium. Int J Oral Sci 5(2):75–84. https://doi.org/10.1038/ijos.2013.41CrossRefPubMedPubMedCentral

10.

Li J, Parada C, Chai Y et al (2017) Cellular and molecular mechanisms of tooth root development. Development 144(3):374–384. https://doi.org/10.1242/dev.137216CrossRefPubMedPubMedCentral

11.

Feng J, Jing J, Li J, Chai Y et al (2017) BMP signaling orchestrates a transcriptional network to control the fate of mesenchymal stem cells in mice. Development 144(14):2560–2569. https://doi.org/10.1242/dev.150136CrossRefPubMedPubMedCentral

12.

Zhang H, Li L, Kesterke MJ, Qin C et al (2019) High-Phosphate Diet Improved the Skeletal Development of Fam20c-Deficient Mice. Cells Tissues Organs 208(1–2):25–36. https://doi.org/10.1159/000506005CrossRefPubMed

13.

Tibaldi E, Brocca A, Sticca A, Brunati AM et al (2020) Fam20C-mediated phosphorylation of osteopontin is critical for its secretion but dispensable for its action as a cytokine in the activation of hepatic stellate cells in liver fibrogenesis. Faseb J 34(1):1122–1135. https://doi.org/10.1096/fj.201900880RCrossRefPubMed

14.

Djoudi Ouadda BA, Gauthier MS, Susan-Resiga D, Seidah NG et al (2019) Ser-Phosphorylation of PCSK9 (Proprotein Convertase Subtilisin-Kexin 9) by Fam20C (Family With Sequence Similarity 20, Member C) Kinase Enhances Its Ability to Degrade the LDLR (Low-Density Lipoprotein Receptor. Arterioscler Thromb Vasc Biol 39(10):1996–2013. https://doi.org/10.1161/ATVBAHA.119.313247CrossRefPubMed

15.

Du EX, Wang XF, Yang WC, Hao JJ et al (2015) Characterization of Fam20C expression in odontogenesis and osteogenesis using transgenic mice. Int J Oral Sci 7(2):89–94. https://doi.org/10.1038/ijos.2014.67CrossRefPubMed

16.

Wang X, Wang S, Lu Y, Qin C et al (2012) FAM20C plays an essential role in the formation of murine teeth. J Biol Chem 287(43):35934–35942. https://doi.org/10.1074/jbc.M112.386862CrossRefPubMedPubMedCentral

17.

Wang X, Jung J, Liu Y, Qin C et al (2013) The specific role of FAM20C in amelogenesis. J Dent Res 92(11):995–1009. https://doi.org/10.1177/0022034513504588CrossRefPubMedPubMedCentral

18.

Liu C, Zhou N, Wang Y, Qin C et al (2018) Abrogation of Fam20c altered cell behaviors and BMP signaling of immortalized dental mesenchymal cells. Exp Cell Res 363(2):188–195. https://doi.org/10.1016/j.yexcr.2018.01.004CrossRefPubMedPubMedCentral

19.

Wang X, Wang J, Yuan B, Qin C et al (2014) Overexpression of Dmp1 fails to rescue the bone and dentin defects in Fam20C knockout mice. Connect Tissue Res 55(4):299–303. https://doi.org/10.3109/03008207.2014.923414CrossRefPubMedPubMedCentral

20.

Liu J, Saiyin W, Xie X, Li L et al (2020) Ablation of Fam20c causes amelogenesis imperfecta via inhibiting Smad dependent BMP signaling pathway. Biol Direct 15(1):16. https://doi.org/10.1186/s13062-020-00270-7CrossRefPubMedPubMedCentral

21.

Wang X, Wang S, Li C, Qin C et al (2012) Inactivation of a novel FGF23 regulator, FAM20C, leads to hypophosphatemic rickets in mice. PLoS Genet 8(5):e1002708. https://doi.org/10.1371/journal.pgen.1002708CrossRefPubMedPubMedCentral

22.

Liu P, Zhang H, Liu C, Qin C et al (2014) Inactivation of Fam20C in cells expressing type I collagen causes periodontal disease in mice. Plos One 9(12):e114396. https://doi.org/10.1371/journal.pone.0114396CrossRefPubMedPubMedCentral

23.

Li L, Saiyin W, Zhang H, Lu Y et al (2019) FAM20A is essential for amelogenesis, but is dispensable for dentinogenesis. J Mol Histol 50(6):581–591. https://doi.org/10.1007/s10735-019-09851-xCrossRefPubMedPubMedCentral

24.

Buzgariu W, Curchod ML, Perruchoud C, Galliot B et al (2020) Combining BrdU-labeling to detection of neuronal markers to monitor adult neurogenesis in hydra. Methods Mol Biol 2047:3–24. https://doi.org/10.1007/978-1-4939-9732-9_1CrossRefPubMed

25.

Lee H, Min SK, Song Y, Park JB et al (2019) Bone morphogenetic protein‑7 upregulates genes associated with osteoblast differentiation, including collagen I, Sp7 and IBSP in gingiva-derived stem cells. Exp Ther Med 18(4):2867–2876. https://doi.org/10.3892/etm.2019.7904CrossRefPubMedPubMedCentral

26.

Ciau-Uitz A, Patient R et al (2019) Gene regulatory networks governing the generation and regeneration of blood. J Comput Biol 26(7):719–725. https://doi.org/10.1089/cmb.2019.0114CrossRefPubMed

27.

Tao H, Lin H, Sun Z, Chen Z et al (2019) Klf4 promotes dentinogenesis and odontoblastic differentiation via modulation of TGF‑β signaling pathway and interaction with histone acetylation. J Bone Miner Res 34(8):1502–1516. https://doi.org/10.1002/jbmr.3716CrossRefPubMed

28.

Elhaddaoui R, Qoraich HS, Bahije L, Zaoui F et al (2017) Orthodontic aligners and root resorption: a systematic review. Int Orthod 15(1):1–12. https://doi.org/10.1016/j.ortho.2016.12.019CrossRefPubMed

29.

Sirotkin AV, Radosová M, Tarko A, Alonso F et al (2020) Effect of morphology and support of copper nanoparticles on basic ovarian granulosa cell functions. Nanotoxicology 32(2):1–13. https://doi.org/10.1080/17435390.2020.1736680CrossRef

30.

Zhang Y, Fang M, Yang Z, Chen W et al (2020) GATA binding protein 4 regulates tooth root dentin development via FBP1. Int J Biol Sci 16(1):181–193. https://doi.org/10.7150/ijbs.36567CrossRefPubMedPubMedCentral

31.

Harkness L, Chen X, Gillard M, Davies AM et al (2019) Media composition modulates human embryonic stem cell morphology and may influence preferential lineage differentiation potential. PLoS ONE 14(3):e213678. https://doi.org/10.1371/journal.pone.0213678CrossRefPubMedPubMedCentral

32.

Somoza RA, Acevedo CA, Albornoz F, Weinstein-Oppenheimer C et al (2017) TGFβ3 secretion by three-dimensional cultures of human dental apical papilla mesenchymal stem cells. J Tissue Eng Regen Med 11(4):1045–1056. https://doi.org/10.1002/term.2004CrossRefPubMed

33.

Jiang Q, Du J, Yin X, Fan Z et al (2013) Shh signaling, negatively regulated by BMP signaling, inhibits the osteo/dentinogenic differentiation potentials of mesenchymal stem cells from apical papilla. Mol Cell Biochem 383(1–2):85–93. https://doi.org/10.1007/s11010-013-1757-9CrossRefPubMed

Titel: Ablation of FAM20C caused short root defects via suppressing the BMP signaling pathway in mice
verfasst von: Lili Li
Peihong Liu
Xuechao Lv
Tianliang Yu
Xingai Jin
Rui Wang
Xiaohua Xie
Qingshan Wang
Yingqun Liu
Wuliji Saiyin
Publikationsdatum: 22.03.2022
Verlag: Springer Medizin
Erschienen in: Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie / Ausgabe 6/2023
Print ISSN: 1434-5293
Elektronische ISSN: 1615-6714
DOI: https://doi.org/10.1007/s00056-022-00386-7

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Ablation of FAM20C caused short root defects via suppressing the BMP signaling pathway in mice

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