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01.03.2011 | Original Article—Liver, Pancreas, and Biliary Tract

Association of hepatitis B virus mutations in basal core promoter and precore regions with severity of liver disease: an investigation of 793 Chinese patients with mild and severe chronic hepatitis B and acute-on-chronic liver failure

verfasst von: Zhihui Xu, Xiaoqiang Ren, Yan Liu, Xiaodong Li, Siyu Bai, Yanwei Zhong, Lin Wang, Panyong Mao, Huifen Wang, Shaojie Xin, Vincent Wai-Sun Wong, Henry Lik-Yuen Chan, Fabien Zoulim, Dongping Xu

Erschienen in: Journal of Gastroenterology | Ausgabe 3/2011

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Abstract

Objective

To investigate the features of hepatitis B virus (HBV) basal core promoter/precore (BCP/PC) mutations and genotypes in a large number of mild/severe chronic hepatitis B (CHB-M/CHB-S), and acute-on-chronic liver failure (ACLF) patients and analyze the clinical implications of the virologic features.

Patients and methods

Sera of 793 (325 CHB-M, 170 CHB-S, and 298 ACLF) patients admitted to or who had visited Beijing 302 Hospital from January 2005 to December 2008 were collected and successfully amplified for the HBV BCP/PC and a 1225-bp-long S/Pol (nt 54–1278) gene regions. Biochemical and serological parameters and HBV DNA level were routinely performed. Viral DNA was extracted and subjected to a nested PCR. Genotypes/subgenotypes were determined based on complete genomic sequence or on analysis of the 1225-bp-long S/Pol-gene sequence. HBV genotyping was performed by direct PCR sequencing followed by molecular evolutionary analysis of the viral sequences. A P value of <0.05 (two-sided) was considered to be statistically significant.

Conclusions

Our findings suggest that CHB patients infected with BCP/PC mutant viruses are more susceptible to severe hepatitis and ACLF than those with the BCP/PC wild-type virus and that ACLF patients with PC mutant viruses have an increased risk of death. As such, the HBV PC mutation is a potential predictive indicator of ACLF outcome.

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Titel: Association of hepatitis B virus mutations in basal core promoter and precore regions with severity of liver disease: an investigation of 793 Chinese patients with mild and severe chronic hepatitis B and acute-on-chronic liver failure
verfasst von: Zhihui Xu
Xiaoqiang Ren
Yan Liu
Xiaodong Li
Siyu Bai
Yanwei Zhong
Lin Wang
Panyong Mao
Huifen Wang
Shaojie Xin
Vincent Wai-Sun Wong
Henry Lik-Yuen Chan
Fabien Zoulim
Dongping Xu
Publikationsdatum: 01.03.2011
Verlag: Springer Japan
Erschienen in: Journal of Gastroenterology / Ausgabe 3/2011
Print ISSN: 0944-1174
Elektronische ISSN: 1435-5922
DOI: https://doi.org/10.1007/s00535-010-0315-4

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Association of hepatitis B virus mutations in basal core promoter and precore regions with severity of liver disease: an investigation of 793 Chinese patients with mild and severe chronic hepatitis B and acute-on-chronic liver failure