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01.08.2012 | Original Article

Immunohistochemical evidence of the prognostic value of hedgehog pathway components in primary gallbladder carcinoma

verfasst von: Jinmao Li, Tao Wu, Jianguo Lu, Yizhan Cao, Nuan Song, Tao Yang, Rui Dong, Yuan Yang, Li Zang, Xilin Du, Shengzhi Wang

Erschienen in: Surgery Today | Ausgabe 8/2012

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Abstract

Purpose

The activation of hedgehog (Hh) pathways has been studied extensively in many malignant tumors to elucidate their clinical diagnostic and prognostic utilities. However, their roles in primary gallbladder carcinoma (GBC) remain poorly understood. This study was conducted to clarify the immunoreactivity and prognostic value of Hh pathway components in GBC.

Methods

Levels of sonic hedgehog (Shh), its receptor, Patched (Ptch1), and its downstream transcription factor, Gli1 protein, were measured by immunohistochemistry in 93 specimens from patients with GBC. We analyzed the correlations between the expression of these factors and clinicopathological features, including prognosis.

Results

Among the 93 GBC specimens, 76 (81.7%), 70 (75.3%) and 66 (70.0%) were positive for Shh, Ptch1 and Gli1 expression, respectively. Expressions were significantly correlated with stage, lymph node metastasis, venous invasion, hepatic infiltration and lymphatic invasion (all P < 0.05). Patients with positive staining for Shh, Ptch1 and Gli1 had significantly lower survival rates than patients with negative staining. The expression patterns of Shh, Ptch1 and Gli1 were all associated with a malignant behavior risk category in GBC.

Conclusions

To our knowledge, this is the first report to define the role of the Hh pathway in GBC. Shh, Ptch1 and Gli1 are frequently expressed in GBC and associated with poorer survival. Thus, high expressions of Shh, Ptch1 and Gli1 proteins could serve as auxiliary parameters for predicting the malignant behavior of GBC.

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Titel: Immunohistochemical evidence of the prognostic value of hedgehog pathway components in primary gallbladder carcinoma
verfasst von: Jinmao Li
Tao Wu
Jianguo Lu
Yizhan Cao
Nuan Song
Tao Yang
Rui Dong
Yuan Yang
Li Zang
Xilin Du
Shengzhi Wang
Publikationsdatum: 01.08.2012
Verlag: Springer Japan
Erschienen in: Surgery Today / Ausgabe 8/2012
Print ISSN: 0941-1291
Elektronische ISSN: 1436-2813
DOI: https://doi.org/10.1007/s00595-012-0157-1

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Immunohistochemical evidence of the prognostic value of hedgehog pathway components in primary gallbladder carcinoma