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Archives of Virology

Erschienen in:

01.06.2013 | Original Article

Simultaneous detection and identification of four viruses infecting pepino by multiplex RT-PCR

verfasst von: Beibei Ge, Qian Li, Guojie Liu, Meiguang Lu, Shifang Li, Hongqing Wang

Erschienen in: Archives of Virology | Ausgabe 6/2013

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Abstract

Potato virus M (PVM), pepino mosaic virus (PepMV), tomato mosaic virus (ToMV), and potato virus S (PVS) infect pepino and cause serious crop losses. In this study, a multiplex RT-PCR method was developed for simultaneous detection and differentiation of PVS, ToMV, PepMV and PVM. The method was highly reliable and sensitive; validation was accomplished by testing pepino samples collected from different regions of China. In this survey, PVM, ToMV and PVS were detected in 37.0 %, 31.0 % and 5.5 % of samples tested, respectively, confirming the widespread occurrence of these three viruses in China. PepMV was not detected in any of the samples, which indicated that this virus may not be prevalent in China. The results suggest that the new multiplex RT-PCR method has potential to be used routinely for surveys of pepino for virus infection.

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Titel: Simultaneous detection and identification of four viruses infecting pepino by multiplex RT-PCR
verfasst von: Beibei Ge
Qian Li
Guojie Liu
Meiguang Lu
Shifang Li
Hongqing Wang
Publikationsdatum: 01.06.2013
Verlag: Springer Vienna
Erschienen in: Archives of Virology / Ausgabe 6/2013
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-013-1604-z

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