nach oben

Clinical Oral Investigations

Erschienen in:

01.07.2014 | Original Article

Oral lichen planus may enhance the expression of Th17-associated cytokines in local lesions of chronic periodontitis

verfasst von: Hui Wang, Qi Han, Zhenhua Luo, Caixia Xu, Jiajia Liu, Hongxia Dan, Yi Xu, Xin Zeng, Qianming Chen

Erschienen in: Clinical Oral Investigations | Ausgabe 6/2014

Einloggen, um Zugang zu erhalten

Abstract

Objectives

This study aims to compare the expression levels of interleukin (IL)-17 and IL-23 in local periodontal tissues from patients with both chronic periodontitis and oral lichen planus (CP-OLP), patients with chronic periodontitis (CP) only, patients with oral lichen planus (OLP) only, and healthy controls (HC).

Materials and methods

The periodontal tissues were collected from 15 CP-OLP patients, 15 CP patients, 15 OLP patients, and 10 healthy controls. Immunohistochemistry (IHC) and real-time quantitative PCR (qPCR) was performed to investigate the protein and mRNA expression level of IL-17 and IL-23 in periodontal lesions from these four groups.

Results

IHC statistical analysis showed that the expression level of IL-17- and IL-23p19-positive cells significantly increased in CP-OLP group compared with that in CP (P < 0.01) and OLP groups (P < 0.05), showing intense staining reaction in local lamina propria lesions. Meanwhile, qPCR result showed higher IL-17 mRNA level in CP-OLP compared with that in CP and OLP groups and demonstrated a significant increase than OLP group (P < 0.05). Moreover, it was found that IL-17 mRNA expression level in erosive CP-OLP patients was significantly correlated with probing depth and attachment loss (P < 0.05).

Conclusions

This study indicated that there was an increased expression level of IL-17 and IL-23 in periodontal tissues from periodontitis patients with oral lichen planus, which might aggravate the inflammatory response in local lesions.

Clinical relevance

Oral lichen planus and chronic periodontitis may have interaction in disease pathogenesis, while IL-17 detection in local lesions may be helpful in identifying the disease severity in periodontitis patients with oral lichen planus.

Sugerman PB, Savage NW, Walsh LJ, Zhao ZZ, Zhou XJ, Khan A, Seymour GJ, Bigby M (2002) The pathogenesis of oral lichen planus. Crit Rev Oral Biol Med 13:350–365PubMedCrossRef

Van Dyke TE, Serhan CN (2003) Resolution of inflammation: a new paradigm for the pathogenesis of periodontal diseases. J Dent Res 82:82–90PubMedCrossRef

Lopez-Jornet P, Camacho-Alonso F (2012) Periodontal conditions in patients with oral lichen planus: a pilot study. Quintessence Int 43:147–152PubMed

Azizi A, Rezaee M (2012) Comparison of periodontal status in gingival oral lichen planus patients and healthy subjects. Dermatol Res Pract 2012:561232PubMedCentralPubMed

Ramon-Fluixa C, Bagan-Sebastian J, Milian-Masanet M, Scully C (1999) Periodontal status in patients with oral lichen planus: a study of 90 cases. Oral Dis 5:303–306PubMedCrossRef

Mignogna MD, Lo RL, Fedele S (2005) Gingival involvement of oral lichen planus in a series of 700 patients. J Clin Periodontol 32:1029–1033PubMedCrossRef

Romagnani S, Maggi E, Liotta F, Cosmi L, Annunziato F (2009) Properties and origin of human Th17 cells. Mol Immunol 47:3–7PubMedCrossRef

Tesmer LA, Lundy SK, Sarkar S, Fox DA (2008) Th17 cells in human disease. Immunol Rev 223:87–113PubMedCentralPubMedCrossRef

Yu JJ, Ruddy MJ, Wong GC, Sfintescu C, Baker PJ, Smith JB, Evans RT, Gaffen SL (2007) An essential role for IL-17 in preventing pathogen-initiated bone destruction: recruitment of neutrophils to inflamed bone requires IL-17 receptor-dependent signals. Blood 109:3794–3802PubMedCentralPubMedCrossRef

10.

Gaffen SL, Hajishengallis G (2008) A new inflammatory cytokine on the block: re-thinking periodontal disease and the Th1/Th2 paradigm in the context of Th17 cells and IL-17. J Dent Res 87:817–828PubMedCentralPubMedCrossRef

11.

Vered M, Furth E, Shalev Y, Dayan D (2012) Inflammatory cells of immunosuppressive phenotypes in oral lichen planus have a proinflammatory pattern of expression and are associated with clinical parameters. Clin Oral Investig 2012

12.

Oppmann B, Lesley R, Blom B, Timans JC, Xu Y, Hunte B et al (2000) Novel p19 protein engages IL-12p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12. Immunity 13:715–725PubMedCrossRef

13.

McGeachy MJ, Cua DJ (2008) Th17 cell differentiation: the long and winding road. Immunity 28:445–453PubMedCrossRef

14.

Kreymborg K, Etzensperger R, Dumoutier L, Haak S, Rebollo A, Buch T, Heppner FL, Renauld JC, Becher B (2007) IL-22 is expressed by Th17 cells in an IL-23-dependent fashion, but not required for the development of autoimmune encephalomyelitis. J Immunol 179:8098–8104PubMedCrossRef

15.

Wang H, Luo Z, Lei L, Sun Z, Zhou M, Dan H, Zeng X, Chen Q (2013) Interaction between oral lichen planus and chronic periodontitis with Th17-associated cytokines in serum. Inflammation 36:696–704PubMedCrossRef

16.

Engebretson S, Chertog R, Nichols A, Hey-Hadavi J, Celenti R, Grbic J (2007) Plasma levels of tumour necrosis factor-alpha in patients with chronic periodontitis and type 2 diabetes. J Clin Periodontol 34:18–24PubMedCrossRef

17.

Detert J, Pischon N, Burmester GR, Buttgereit F (2010) The association between rheumatoid arthritis and periodontal disease. Arthritis Res Ther 12:218PubMedCentralPubMedCrossRef

18.

Kobayashi T, Murasawa A, Komatsu Y, Yokoyama T, Ishida K, Abe A, Yamamoto K, Yoshie H (2010) Serum cytokine and periodontal profiles in relation to disease activity of rheumatoid arthritis in Japanese adults. J Periodontol 81:650–657PubMedCrossRef

19.

Queiroz-Junior CM, Madeira MF, Coelho FM, Costa VV, Bessoni RL, Sousa LF, Garlet GP, Souza DG, Teixeira MM, Silva TA (2011) Experimental arthritis triggers periodontal disease in mice: involvement of TNF-alpha and the oral microbiota. J Immunol 187:3821–3830PubMedCrossRef

20.

Kalogerakou F, Albanidou-Farmaki E, Markopoulos AK, Antoniades DZ (2008) Detection of T cells secreting type 1 and type 2 cytokines in the peripheral blood of patients with oral lichen planus. Hippokratia 12:230–235PubMedCentralPubMed

21.

Rhodus NL, Cheng B, Bowles W, Myers S, Miller L, Ondrey F (2006) Proinflammatory cytokine levels in saliva before and after treatment of (erosive) oral lichen planus with dexamethasone. Oral Dis 12:112–116PubMedCrossRef

22.

Roopashree MR, Gondhalekar RV, Shashikanth MC, George J, Thippeswamy SH, Shukla A (2010) Pathogenesis of oral lichen planus—a review. J Oral Pathol Med 39:729–734PubMedCrossRef

23.

Pekiner FN, Demirel GY, Borahan MO, Ozbayrak S (2012) Cytokine profiles in serum of patients with oral lichen planus. Cytokine 60:701–706PubMedCrossRef

24.

Liu W, Dan H, Wang Z, Jiang L, Zhou Y, Zhao M, Chen Q, Zeng X (2009) IFN-gamma and IL-4 in saliva of patients with oral lichen planus: a study in an ethnic Chinese population. Inflammation 32:176–181PubMedCrossRef

25.

Zhang Y, Lin M, Zhang S, Wang Z, Jiang L, Shen J, Chen Q (2008) NF-kappaB-dependent cytokines in saliva and serum from patients with oral lichen planus: a study in an ethnic Chinese population. Cytokine 41:144–149PubMedCrossRef

26.

Dan H, Liu W, Zhou Y, Wang J, Chen Q, Zeng X (2010) Association of interleukin-8 gene polymorphisms and haplotypes with oral lichen planus in a Chinese population. Inflammation 33:76–81PubMedCrossRef

27.

Bai J, Jiang L, Lin M, Zeng X, Wang Z, Chen Q (2009) Association of polymorphisms in the tumor necrosis factor-alpha and interleukin-10 genes with oral lichen planus: a study in a Chinese cohort with Han ethnicity. J Interferon Cytokine Res 29:381–388PubMedCrossRef

28.

Dan H, Liu W, Wang J, Wang Z, Wu R, Chen Q, Zeng X, Zhou Y (2011) Elevated IL-10 concentrations in serum and saliva from patients with oral lichen planus. Quintessence Int 42:157–163PubMed

29.

Bai J, Zhang Y, Lin M, Zeng X, Wang Z, Shen J, Jiang L, Gao F, Chen Q (2007) Interleukin-18 gene polymorphisms and haplotypes in patients with oral lichen planus: a study in an ethnic Chinese cohort. Tissue Antigens 70:390–397PubMedCrossRef

30.

Karagouni EE, Dotsika EN, Sklavounou A (1994) Alteration in peripheral blood mononuclear cell function and serum cytokines in oral lichen planus. J Oral Pathol Med 23:28–35PubMedCrossRef

31.

Rhodus NL, Cheng B, Ondrey F (2007) Th1/Th2 cytokine ratio in tissue transudates from patients with oral lichen planus. Mediators Inflamm 2007:19854PubMedCentralPubMedCrossRef

32.

Piccinni MP, Lombardelli L, Logiodice F, Tesi D, Kullolli O, Biagiotti R, Giudizi M, Romagnani S, Maggi E, Ficarra G (2013) Potential pathogenetic role of Th17, Th0, and Th2 cells in erosive and reticular oral lichen planus. Oral Dis. doi:10.1111/odi.12094 PubMed

33.

Trombone AP, Claudino M, Colavite P, de Assis GF, Avila-Campos MJ, Silva JS, Campanelli AP, Ibanez OM, De Franco M, Garlet GP (2010) Periodontitis and arthritis interaction in mice involves a shared hyper-inflammatory genotype and functional immunological interferences. Genes Immun 11:479–489PubMedCrossRef

34.

Lester SR, Bain JL, Johnson RB, Serio FG (2007) Gingival concentrations of interleukin-23 and -17 at healthy sites and at sites of clinical attachment loss. J Periodontol 78:1545–1550PubMedCrossRef

35.

Gemmell E, Yamazaki K, Seymour GJ (2002) Destructive periodontitis lesions are determined by the nature of the lymphocytic response. Crit Rev Oral Biol Med 13:17–34PubMedCrossRef

36.

Yago T, Nanke Y, Ichikawa N, Kobashigawa T, Mogi M, Kamatani N, Kotake S (2009) IL-17 induces osteoclastogenesis from human monocytes alone in the absence of osteoblasts, which is potently inhibited by anti-TNF-alpha antibody: a novel mechanism of osteoclastogenesis by IL-17. J Cell Biochem 108:947–955PubMedCrossRef

37.

Sato K, Suematsu A, Okamoto K, Yamaguchi A, Morishita Y, Kadono Y et al (2006) Th17 functions as an osteoclastogenic helper T cell subset that links T cell activation and bone destruction. J Exp Med 203:2673–2682PubMedCentralPubMedCrossRef

Titel: Oral lichen planus may enhance the expression of Th17-associated cytokines in local lesions of chronic periodontitis
verfasst von: Hui Wang
Qi Han
Zhenhua Luo
Caixia Xu
Jiajia Liu
Hongxia Dan
Yi Xu
Xin Zeng
Qianming Chen
Publikationsdatum: 01.07.2014
Verlag: Springer Berlin Heidelberg
Erschienen in: Clinical Oral Investigations / Ausgabe 6/2014
Print ISSN: 1432-6981
Elektronische ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-013-1131-z

Update Zahnmedizin

Bestellen Sie unseren kostenlosen Newsletter und bleiben Sie gut informiert – ganz bequem per eMail.

Newsletter bestellen

Live-Webinar "Urologie und Sexualmedizin in der Praxis"

Springer Medizin

Oral lichen planus may enhance the expression of Th17-associated cytokines in local lesions of chronic periodontitis

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

Neu im Fachgebiet Zahnmedizin

„Übersichtlicher Wegweiser“: Lauterbachs umstrittener Klinik-Atlas ist online

Klinikreform soll zehntausende Menschenleben retten

Darf man die Behandlung eines Neonazis ablehnen?

Ein Drittel der jungen Ärztinnen und Ärzte erwägt abzuwandern

Update Zahnmedizin

Live-Webinar "Urologie und Sexualmedizin in der Praxis"

Springer Medizin

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 6/2014

TGF-βRI kinase activity mediates Emdogain-stimulated in vitro osteoclastogenesis

Association between recurrent aphthous stomatitis and Helicobacter pylori infection: a meta-analysis

Bleaching effectiveness, hydrogen peroxide diffusion, and cytotoxicity of a chemically activated bleaching gel

Anabolic steroids affect human periodontal health and microbiota

The Basic Erosive Wear Examination (BEWE) applied retrospectively to two studies

Polymerization kinetic calculations in dental composites: a method comparison analysis

Neu im Fachgebiet Zahnmedizin

„Übersichtlicher Wegweiser“: Lauterbachs umstrittener Klinik-Atlas ist online

Klinikreform soll zehntausende Menschenleben retten

Darf man die Behandlung eines Neonazis ablehnen?

Ein Drittel der jungen Ärztinnen und Ärzte erwägt abzuwandern

Update Zahnmedizin