nach oben

Clinical Oral Investigations

Erschienen in:

11.07.2017 | Original Article

Biofunctionalization of porcine-derived collagen matrix using enamel matrix derivative and platelet-rich fibrin: influence on mature endothelial cell characteristics in vitro

verfasst von: Jung Soo Park, Andreas Max Pabst, Maximilian Ackermann, Maximilian Moergel, Junho Jung, Adrian Kasaj

Erschienen in: Clinical Oral Investigations | Ausgabe 2/2018

Einloggen, um Zugang zu erhalten

Abstract

Objectives

The present study evaluated the effect of an enamel matrix derivative (EMD) and platelet-rich fibrin (PRF)-modified porcine-derived collagen matrix (PDCM) on human umbilical vein endothelial cells (HUVEC) in vitro.

Materials and methods

PDCM (mucoderm®) was prepared to 6 mm (±0.1 mm) diameter discs. PDCM samples were incubated with either EMD, PRF, or control solutions for 100 min at 4 °C before the experiments. Cell-inducing properties of test materials on HUVEC cells were tested with cell proliferation assays (MTT, PrestoBlue®), a cytotoxicity assay (ToxiLight®), a Boyden chamber migration assay, and a cell attachment assay. Scanning electron microscopy (SEM) imaging was performed to determine the surface and the architecture of the modified matrices.

Results

Cell proliferation was elevated in the EMD and PRF groups compared with control (p each ≤0.046). PRF modification increased HUVEC migration ability by 8-fold compared with both control and EMD groups (p each <0.001). Both treatments significantly promoted the cell attachment of HUVEC to PDCM, as assessed by direct cell counts on the matrices (p each <0.001).

Conclusions

HUVEC cell characteristics were overall improved by EMD- and PRF- modified PDCM. Adsorbed bioactive molecules to the PDCM surface may have contributed to a more preferable environment to surrounding cells.

Clinical relevance

The results may give evidence that PDCM modification with EMD or PRF, respectively, might be a useful approach to improve clinical outcomes, to prevent inflammatory reactions and wound-healing disturbances, and to expand the clinical application area of PDCM.

Nur mit Berechtigung zugänglich

Pabst AM, Happe A, Callaway A, Ziebart T, Stratul SI, Ackermann M, Konerding MA, Willershausen B, Kasaj A (2014) In vitro and in vivo characterization of porcine acellular dermal matrix for gingival augmentation procedures. J Periodontal Res 49(3):371–381. doi:10.1111/jre.12115 CrossRefPubMed

Atieh MA, Alsabeeha N, Tawse-Smith A, Payne AG (2015) Xenogeneic collagen matrix for periodontal plastic surgery procedures: a systematic review and meta-analysis. J Periodontal Res. doi:10.1111/jre.12333

Kim Y, Ko H, Kwon IK, Shin K (2016) Extracellular matrix revisited: roles in tissue engineering. Int Neurourol J 20(Suppl 1):S23–S29. doi:10.5213/inj.1632600.318 CrossRefPubMedPubMedCentral

Hammarstrom L (1997) Enamel matrix, cementum development and regeneration. J Clin Periodontol 24(9 Pt 2):658–668CrossRefPubMed

Gestrelius S, Andersson C, Lidstrom D, Hammarstrom L, Somerman M (1997) In vitro studies on periodontal ligament cells and enamel matrix derivative. J Clin Periodontol 24(9 Pt 2):685–692CrossRefPubMed

Miron RJ, Sculean A, Cochran DL, Froum S, Zucchelli G, Nemcovsky C, Donos N, Lyngstadaas SP, Deschner J, Dard M, Stavropoulos A, Zhang Y, Trombelli L, Kasaj A, Shirakata Y, Cortellini P, Tonetti M, Rasperini G, Jepsen S, Bosshardt DD (2016) 20 years of enamel matrix derivative: the past, the present and the future. J Clin Periodontol. doi:10.1111/jcpe.12546

Koop R, Merheb J, Quirynen M (2012) Periodontal regeneration with enamel matrix derivative in reconstructive periodontal therapy: a systematic review. J Periodontol 83(6):707–720. doi:10.1902/jop.2011.110266 CrossRefPubMed

McGuire MK, Scheyer ET, Nunn M (2012) Evaluation of human recession defects treated with coronally advanced flaps and either enamel matrix derivative or connective tissue: comparison of clinical parameters at 10 years. J Periodontol 83(11):1353–1362. doi:10.1902/jop.2012.110373 CrossRefPubMed

Bosshardt DD (2008) Biological mediators and periodontal regeneration: a review of enamel matrix proteins at the cellular and molecular levels. J Clin Periodontol 35(8 Suppl):87–105. doi:10.1111/j.1600-051X.2008.01264.x CrossRefPubMed

10.

Miron RJ, Dard M, Weinreb M (2015) Enamel matrix derivative, inflammation and soft tissue wound healing. J Periodontal Res 50(5):555–569. doi:10.1111/jre.12245 CrossRefPubMed

11.

Choukroun J, Adda F, Schoeffler C, Vervelle A (2001) Une opportunite′ en paro-implantologie: le PRF. Implantodontie 42:55–62 French

12.

Dohan DM, Choukroun J, Diss A, Dohan SL, Dohan AJ, Mouhyi J, Gogly B (2006) Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part II: Platelet-related biologic features. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 101(3):e45–e50. doi:10.1016/j.tripleo.2005.07.009 CrossRefPubMed

13.

Dohan DM, Choukroun J, Diss A, Dohan SL, Dohan AJ, Mouhyi J, Gogly B (2006) Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part I: Technological concepts and evolution. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 101(3):e37–e44. doi:10.1016/j.tripleo.2005.07.008 CrossRefPubMed

14.

Schar MO, Diaz-Romero J, Kohl S, Zumstein MA, Nesic D (2015) Platelet-rich concentrates differentially release growth factors and induce cell migration in vitro. Clin Orthop Relat Res 473(5):1635–1643. doi:10.1007/s11999-015-4192-2 CrossRefPubMedPubMedCentral

15.

Marenzi G, Riccitiello F, Tia M, di Lauro A, Sammartino G (2015) Influence of leukocyte- and platelet-rich fibrin (L-PRF) in the healing of simple postextraction sockets: a split-mouth study. Biomed Res Int 2015:369273. doi:10.1155/2015/369273 CrossRefPubMedPubMedCentral

16.

Femminella B, Iaconi MC, Di Tullio M, Romano L, Sinjari B, D'Arcangelo C, De Ninis P, Paolantonio M (2016) Clinical comparison of platelet-rich fibrin and a gelatin sponge in the management of palatal wounds after epithelialized free gingival graft harvest: a randomized clinical trial. J Periodontol 87(2):103–113. doi:10.1902/jop.2015.150198 CrossRefPubMed

17.

Kobayashi E, Fluckiger L, Fujioka-Kobayashi M, Sawada K, Sculean A, Schaller B, Miron RJ (2016) Comparative release of growth factors from PRP, PRF, and advanced-PRF. Clin Oral Investig 20(9):2353–2360. doi:10.1007/s00784-016-1719-1 CrossRefPubMed

18.

Folkman J (1971) Tumor angiogenesis: therapeutic implications. N Engl J Med 285(21):1182–1186. doi:10.1056/NEJM197111182852108 CrossRefPubMed

19.

Germain S, Monnot C, Muller L, Eichmann A (2010) Hypoxia-driven angiogenesis: role of tip cells and extracellular matrix scaffolding. Curr Opin Hematol 17(3):245–251. doi:10.1097/MOH.0b013e32833865b9 PubMed

20.

Michaelis UR (2014) Mechanisms of endothelial cell migration. Cell Mol Life Sci 71(21):4131–4148. doi:10.1007/s00018-014-1678-0 CrossRefPubMed

21.

Zaidel-Bar R, Cohen M, Addadi L, Geiger B (2004) Hierarchical assembly of cell-matrix adhesion complexes. Biochem Soc Trans 32(Pt3):416–420. doi:10.1042/BST0320416 CrossRefPubMed

22.

Giancotti FG (1997) Integrin signaling: specificity and control of cell survival and cell cycle progression. Curr Opin Cell Biol 9(5):691–700CrossRefPubMed

23.

Kasaj A, Meister J, Lehmann K, Stratul SI, Schlee M, Stein JM, Willershausen B, Schmidt M (2012) The influence of enamel matrix derivative on the angiogenic activity of primary endothelial cells. J Periodontal Res 47(4):479–487. doi:10.1111/j.1600-0765.2011.01456.x CrossRefPubMed

24.

Bertl K, An N, Bruckmann C, Dard M, Andrukhov O, Matejka M, Rausch-Fan X (2009) Effects of enamel matrix derivative on proliferation/viability, migration, and expression of angiogenic factor and adhesion molecules in endothelial cells in vitro. J Periodontol 80(10):1622–1630. doi:10.1902/jop.2009.090157 CrossRefPubMed

25.

Gestrelius S, Andersson C, Johansson AC, Persson E, Brodin A, Rydhag L, Hammarstrom L (1997) Formulation of enamel matrix derivative for surface coating. Kinetics and cell colonization. J Clin Periodontol 24(9 Pt 2):678–684CrossRefPubMed

26.

Miron RJ, Bosshardt DD, Laugisch O, Dard M, Gemperli AC, Buser D, Gruber R, Sculean A (2013) In vitro evaluation of demineralized freeze-dried bone allograft in combination with enamel matrix derivative. J Periodontol 84(11):1646–1654. doi:10.1902/jop.2013.120574 PubMed

27.

Lyngstadaas SP, Wohlfahrt JC, Brookes SJ, Paine ML, Snead ML, Reseland JE (2009) Enamel matrix proteins; old molecules for new applications. Orthod Craniofac Res 12(3):243–253. doi:10.1111/j.1601-6343.2009.01459.x CrossRefPubMedPubMedCentral

28.

Philippart P, Daubie V, Pochet R (2005) Sinus grafting using recombinant human tissue factor, platelet-rich plasma gel, autologous bone, and anorganic bovine bone mineral xenograft: histologic analysis and case reports. Int J Oral Maxillofac Implants 20(2):274–281PubMed

29.

Mazor Z, Peleg M, Garg AK, Luboshitz J (2004) Platelet-rich plasma for bone graft enhancement in sinus floor augmentation with simultaneous implant placement: patient series study. Implant Dent 13(1):65–72CrossRefPubMed

30.

Thor A, Wannfors K, Sennerby L, Rasmusson L (2005) Reconstruction of the severely resorbed maxilla with autogenous bone, platelet-rich plasma, and implants: 1-year results of a controlled prospective 5-year study. Clin Implant Dent Relat Res 7(4):209–220CrossRefPubMed

31.

Yang D, Lu X, Hong Y, Xi T, Zhang D (2013) The molecular mechanism of mediation of adsorbed serum proteins to endothelial cells adhesion and growth on biomaterials. Biomaterials 34(23):5747–5758. doi:10.1016/j.biomaterials.2013.04.028 CrossRefPubMed

32.

Okubo K, Kobayashi M, Takiguchi T, Takada T, Ohazama A, Okamatsu Y, Hasegawa K (2003) Participation of endogenous IGF-I and TGF-beta 1 with enamel matrix derivative-stimulated cell growth in human periodontal ligament cells. J Periodontal Res 38(1):1–9CrossRefPubMed

33.

Nikitovic D, Chalkiadaki G, Berdiaki A, Aggelidakis J, Katonis P, Karamanos NK, Tzanakakis GN (2011) Lumican regulates osteosarcoma cell adhesion by modulating TGFbeta2 activity. Int J Biochem Cell Biol 43(6):928–935. doi:10.1016/j.biocel.2011.03.008 CrossRefPubMed

34.

Warstat K, Meckbach D, Weis-Klemm M, Hack A, Klein G, de Zwart P, Aicher WK (2010) TGF-beta enhances the integrin alpha2beta1-mediated attachment of mesenchymal stem cells to type I collagen. Stem Cells Dev 19(5):645–656. doi:10.1089/scd.2009.0208 CrossRefPubMed

35.

Curtis A, Wilkinson C (1997) Topographical control of cells. Biomaterials 18(24):1573–1583CrossRefPubMed

36.

Ranganathan AT, Chandran CR (2014) Platelet-rich fibrin in the treatment of periodontal bone defects. J Contemp Dent Pract 15(3):372–375CrossRefPubMed

37.

Pankov R, Yamada KM (2002) Fibronectin at a glance. J Cell Sci 115(Pt 20):3861–3863CrossRefPubMed

38.

Martino MM, Tortelli F, Mochizuki M, Traub S, Ben-David D, Kuhn GA, Muller R, Livne E, Eming SA, Hubbell JA (2011) Engineering the growth factor microenvironment with fibronectin domains to promote wound and bone tissue healing. Sci Transl Med 3(100):100ra189. doi:10.1126/scitranslmed.3002614 CrossRef

39.

Miron RJ, Bosshardt DD, Buser D, Zhang Y, Tugulu S, Gemperli A, Dard M, Caluseru OM, Chandad F, Sculean A (2015) Comparison of the capacity of enamel matrix derivative gel and enamel matrix derivative in liquid formulation to adsorb to bone grafting materials. J Periodontol 86(4):578–587. doi:10.1902/jop.2015.140538 CrossRefPubMed

40.

Zhang Y, Jing D, Buser D, Sculean A, Chandad F, Miron RJ (2016) Bone grafting material in combination with Osteogain for bone repair: a rat histomorphometric study. Clin Oral Investig 20(3):589–595. doi:10.1007/s00784-015-1532-2 CrossRefPubMed

41.

Miron RJ, Fujioka-Kobayashi M, Zhang Y, Sculean A, Pippenger B, Shirakata Y, Kandalam U, Hernandez M (2016) Osteogain(R) loaded onto an absorbable collagen sponge induces attachment and osteoblast differentiation of ST2 cells in vitro. Clin Oral Investig. doi:10.1007/s00784-016-2019-5

Titel: Biofunctionalization of porcine-derived collagen matrix using enamel matrix derivative and platelet-rich fibrin: influence on mature endothelial cell characteristics in vitro
verfasst von: Jung Soo Park
Andreas Max Pabst
Maximilian Ackermann
Maximilian Moergel
Junho Jung
Adrian Kasaj
Publikationsdatum: 11.07.2017
Verlag: Springer Berlin Heidelberg
Erschienen in: Clinical Oral Investigations / Ausgabe 2/2018
Print ISSN: 1432-6981
Elektronische ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-017-2170-7

Bestellen Sie unseren kostenlosen Newsletter Update Zahnmedizin und bleiben Sie gut informiert – ganz bequem per eMail.

Newsletter bestellen

Die Highlights vom Kongress des American College of Cardiology 2024

Springer Medizin

Biofunctionalization of porcine-derived collagen matrix using enamel matrix derivative and platelet-rich fibrin: influence on mature endothelial cell characteristics in vitro