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01.09.2015 | Original Article

Increased cell proliferation and differential protein expression induced by low-level Er:YAG laser irradiation in human gingival fibroblasts: proteomic analysis

verfasst von: Mayumi Ogita, Sachio Tsuchida, Akira Aoki, Mamoru Satoh, Sayaka Kado, Masanori Sawabe, Hiromi Nanbara, Hiroaki Kobayashi, Yasuo Takeuchi, Koji Mizutani, Yoshiyuki Sasaki, Fumio Nomura, Yuichi Izumi

Erschienen in: Lasers in Medical Science | Ausgabe 7/2015

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Abstract

Erbium-doped yttrium aluminum garnet (Er:YAG) laser treatment has demonstrated favorable wound healing effect after periodontal therapy. One of the reasons may be the positive biological effect of the low-level laser on the irradiated tissues, although the mechanism remains unclear. The aim of this study was to investigate the effect of low-level Er:YAG laser irradiation on cell proliferation and laser-induced differential expression of proteins in human gingival fibroblasts (HGFs) by proteomic analysis. In the first experiment, HGFs were exposed to low-level Er:YAG laser irradiation and the laser-induced cell proliferation and damage were evaluated on day 3. In the second experiment, proteomic analysis was performed on day 1 after irradiation. The peptides prepared from HGFs were analyzed by a hybrid ion trap-Fourier transform mass spectrometer, Mascot search engine, and UniProtKB database. A significant increase in cell proliferation without cell damage after irradiation was observed. Among the total identified 377 proteins, 59 proteins, including galectin-7, which was associated with the process of wound healing, were upregulated and 15 proteins were downregulated in laser-treated HGFs. In the third experiment, the increase in messenger RNA (mRNA) and protein expression of galectin-7 in the irradiated HGFs was validated by various analytical techniques. In addition, the effect of recombinant human galectin-7 on the modulation of HGFs proliferation was confirmed. The results indicate that low-level Er:YAG laser irradiation can promote HGF proliferation and induce a significant change in protein expression and the upregulation of galectin-7 expression may partly contribute to the increase in cell proliferation.

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Titel: Increased cell proliferation and differential protein expression induced by low-level Er:YAG laser irradiation in human gingival fibroblasts: proteomic analysis
verfasst von: Mayumi Ogita
Sachio Tsuchida
Akira Aoki
Mamoru Satoh
Sayaka Kado
Masanori Sawabe
Hiromi Nanbara
Hiroaki Kobayashi
Yasuo Takeuchi
Koji Mizutani
Yoshiyuki Sasaki
Fumio Nomura
Yuichi Izumi
Publikationsdatum: 01.09.2015
Verlag: Springer London
Erschienen in: Lasers in Medical Science / Ausgabe 7/2015
Print ISSN: 0268-8921
Elektronische ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-014-1691-4

Die Highlights vom Kongress des American College of Cardiology 2024

Springer Medizin

Increased cell proliferation and differential protein expression induced by low-level Er:YAG laser irradiation in human gingival fibroblasts: proteomic analysis

Abstract

Die Highlights vom Kongress des American College of Cardiology 2024

Springer Medizin

Abstract

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