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Journal of Assisted Reproduction and Genetics

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01.10.2007 | Original Papers

The effects of age on DNA fragmentation, chromatin packaging and conventional semen parameters in spermatozoa of oligoasthenoteratozoospermic patients

verfasst von: Konstantina Plastira, Pavlos Msaouel, Roxani Angelopoulou, Kyriaki Zanioti, Aris Plastiras, Alexios Pothos, Stamatis Bolaris, Nikolaos Paparisteidis, Dimitris Mantas

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 10/2007

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Abstract

Purpose

To investigate the effects of male ageing on DNA fragmentation and chromatin packaging in the spermatozoa of oligoasthenoteratozoospermic (OAT) patients.

Methods

Sixty-one OAT patients and 49 men with proven fertility (controls) were included in the present study. DNA fragmentation was detected by terminal deoxynucleotidyl transferase-mediated dUTP-nick end labelling (TUNEL) assay, while chromatin packaging was assessed by chromomycin A₃ (CMA₃) staining.

Results

In the patient group, semen volume, percentage of normally shaped spermatozoa and sperm motility decreased significantly (P < 0.05) with age, while sperm concentration and the percentage of TUNEL and CMA₃ positive spermatozoa showed a statistically significant increase with age (P < 0.05). In the control group, conventional semen parameters as well as DNA fragmentation and chromatin packaging did not show a statistically significant change with age (P > 0.05).

Conclusion

Increased age in OAT patients is associated with an increase in sperm concentration, DNA fragmentation and poor chromatin packaging, as well as a decline in semen volume, sperm morphology and motility.

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Titel: The effects of age on DNA fragmentation, chromatin packaging and conventional semen parameters in spermatozoa of oligoasthenoteratozoospermic patients
verfasst von: Konstantina Plastira
Pavlos Msaouel
Roxani Angelopoulou
Kyriaki Zanioti
Aris Plastiras
Alexios Pothos
Stamatis Bolaris
Nikolaos Paparisteidis
Dimitris Mantas
Publikationsdatum: 01.10.2007
Verlag: Springer US
Erschienen in: Journal of Assisted Reproduction and Genetics / Ausgabe 10/2007
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-007-9162-5

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The effects of age on DNA fragmentation, chromatin packaging and conventional semen parameters in spermatozoa of oligoasthenoteratozoospermic patients