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01.06.2011 | Research Article

A three-dimensional cell biology model of human hepatocellular carcinoma in vitro

verfasst von: Jianhua Tang, Jiefeng Cui, Rongxin Chen, Kun Guo, Xiaonan Kang, Yan Li, Dongmei Gao, Lu Sun, Changde Xu, Jie Chen, Zhaoyou Tang, Yinkun Liu

Erschienen in: Tumor Biology | Ausgabe 3/2011

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Abstract

We established an in vitro 3-D model of metastatic hepatocellular carcinoma (HCC) by culturing MHCC97H cells on molecular scaffolds within a rotating wall vessel bioreactor. Morphological and biochemical analyses revealed that the 3-D HCC model mirrored many clinical pathological features of HCC in vivo, including cancer cell morphology, tissue ultrastructure, protein production and secretion, glucose metabolism, tissue-specific gene expression, and apoptosis. Xenografts into livers of nude mice resulted in tumorigenesis and distant metastasis. This 3-D HCC spheroid is a promising model for HCC tumor biology, anticancer drug screening, and for the establishment of HCC animal models.

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Titel: A three-dimensional cell biology model of human hepatocellular carcinoma in vitro
verfasst von: Jianhua Tang
Jiefeng Cui
Rongxin Chen
Kun Guo
Xiaonan Kang
Yan Li
Dongmei Gao
Lu Sun
Changde Xu
Jie Chen
Zhaoyou Tang
Yinkun Liu
Publikationsdatum: 01.06.2011
Verlag: Springer Netherlands
Erschienen in: Tumor Biology / Ausgabe 3/2011
Print ISSN: 1010-4283
Elektronische ISSN: 1423-0380
DOI: https://doi.org/10.1007/s13277-010-0140-7

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