Introduction
In the last few decades, carbapenem-resistant Enterobacteriaceae (CREs) have been regarded as one of the fatal medical threats to public health based on the World Health Organization priority list of antibiotic-resistant bacteria, which could cause a variety of intractable infections, such as pneumonia, bloodstream infections, and urinary tract infections [
1,
2]. Carbapenem-resistant
Klebsiella pneumoniae (CRKP), the most common pathogen amidst the various strains of CREs, is considered as a nationwide clinical therapeutic challenge in China [
3,
4]. According to the corresponding statistics from the China Antimicrobial Surveillance Network, rapidly increasing incidence and prevalence rates of CRKP infection have been observed from 2.9% in 2005 to 25% in 2021 [
5].
K. pneumoniae carbapenemase (KPC) is the most clinically common carbapenemase in CRKP strains in China [
6‐
8].
Only a few available antimicrobial agents show adequate clinical efficacy in treating CRKP infection because of its multidrug resistance, such as aminoglycosides, carbapenems (only used with high dose and prolonged infusion time), tigecycline, and fosfomycin. It is crucial to determine whether combinations of the aforementioned drugs show adequate synergies to achieve bactericidal effects against CRKP [
2,
9,
10].
Moreover, some novel antimicrobial agents have been developed for the sake of overcoming the treating dilemma of CRKP infection in recent years. It is acknowledged that ceftazidime/avibactam (CAZ/AVI) and polymyxins [polymyxin B (PMB) & colistin] reveal their own antibacterial effects as the effective agents against CRKP infection [
2,
9,
10]. As far as we know, clinical studies about comparing clinical efficacy between CAZ/AVI and polymyxin-based therapeutic regimens are still rare [
5,
11]. Consequently, it is worthwhile conducting several further clinical investigations to provide sufficient evidence for making guidelines on treatment of CRKP infection with CAZ/AVI and PMB-based therapeutic regimens.
In a previous study, we found that using a combination treatment scheme of CAZ/AVI with carbapenems, fosfomycin, or tigecycline could significantly decrease the mortality of critically ill patients with CRKP infection [
12]. However, PMB-based treatment regimen were not evaluated. Hence, the current study compares the clinical efficacy and safety of treating CRKP infection in critical ill patients between CAZ/AVI-based and PMB-based regimen.
Methods
Study Design and Participants
This retrospective cohort study was performed at two tertiary hospitals in China and is based upon the ethical standards of the Declaration of Helsinki 1964 and its later amendments or comparable ethical standards. Our study was approved by the Institutional Review Board of Huashan Hospital Affiliated to Fudan University and Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine. All complete data were extracted respectively from the electronic medical record information system in each hospital without direct interaction with the enrolled participants.
Adult patients (age 18 years or over) who were admitted to the intensive care unit (ICU) from January 2019 to October 2021 and received at least one dose of CZA/AVI or PMB for empirical or definitive treatment with verified CRKP infections (based on microbiological culture test) and documented susceptibility testing results were enrolled in our study. The exclusion criteria were as follows: (1) patients who received CAZ/AVI-based or PMB-based treatment for less than 24 h or died within this period; and (2) patients with missing data.
Antibiotic Dosing Regimens
CAZ/AVI-based therapy was considered as an antimicrobial treatment with CAZ/AVI and any other antibiotics except for PMB. Correspondingly, PMB-based therapy was classified as an antimicrobial treatment with PMB and any other antibiotics except for CAZ/AVI. Combination therapy was defined as using any other anti-CRKP agents together with CAZ/AVI or PMB at the onset of CAZ/AVI or PMB treatment, respectively. The selection of CAZ/AVI-based and PMB-based therapy as well as concomitant antibiotics and their duration was at the discretion of the clinicians.
As for the dose regimen of CAZ/AVI, a 2.5-g fixed dose was administered every 8 h with a 2-h infusion time. Dose adjustment was in accordance with patients’ creatinine clearance (CrCl) level. Patients who underwent continuous renal replacement therapy (CRRT) received a standard dosing regimen regardless of the different modes of CRRT [
13].
In the PMB-based therapy group, patients received a loading dose of 2.0–2.5 mg/kg/day and maintenance doses of 1.25–1.5 mg/kg/day every 12 h. Both loading dose and maintenance dose were calculated on the basis of total body weight (TBW) and administered with at least 1-h infusion time. No renal function-based dose adjustment was performed in our study, even if patients were receiving CRRT [
14].
Study Objectives and Variables
The 30-day mortality rate was classified as primary outcome in the current study, and the 30-day microbiological eradication rate was evaluated to compare the clinical efficacy between CAZ/AVI- and PMB-based therapy. Microbiological eradication was determined as the disappearance of CRKP from all subsequent cultures.
Variables that were recorded in our study included age, sex, TBW, site of infection (defined in line with the Centers for Disease Control and Prevention (CDC) criteria [
15]); polymicrobial infections; Sequential Organ Failure Assessment (SOFA) [
16] and Acute Physiology and Chronic Health Evaluation II (APACHE II) scores at the onset of CAZ/AVI-based or PMB-based treatment [
17]; sepsis (identified by SOFA scores ≥ 2 [
16]) when starting CAZ/AVI-based or PMB-based therapy; CrCl (calculated by Cockcroft–Gault formula [
18]) at the beginning of CAZ/AVI-based or PMB-based therapy; CRRT or extracorporeal membrane oxygenation (ECMO) within the duration of CAZ/AVI-based or PMB-based therapy; length of ICU stay before starting antimicrobial therapy; combination therapy and concomitant antibiotic treatment; concomitant use of vasoactive drugs or mechanical ventilation by the start of CAZ/AVI-based or PMB-based therapy; Charlson comorbidity index (CCI) score [
19] and comorbidities at admission; CAZ/AVI-based or PMB-based treatment duration.
Microbiology
All pathogen isolation and antimicrobial susceptibility tests, except for CAZ/AVI, were performed by the Vitek 2 Compact system (bioMérieux, Inc.). The susceptibility of CAZ/AVI was determined by the disk-diffusion method (Kirby–Bauer method); the diameter of inhibition zone > 21 mm and < 20 mm meant susceptibility and resistance, respectively. The Clinical and Laboratory Standards Institute (CLSI) criteria 2020 were utilized as the evaluation standard of breakpoints to interpret all antibiotic susceptibility testing results. In addition, carbapenem resistance was defined as a minimum inhibitory concentration (MIC) of imipenem or meropenem of 4 mg/L or over [
20].
Statistical Analysis
All statistical analyses were performed with SPSS software (version 26.0, IBM Corp, Armonk, NY, USA). The Shapiro–Wilk test was carried out to validate the normality of the distribution of each variable. As for the categorical variables, Pearson’s chi-square test or Fisher’s exact test was utilized for data analysis and calculation of P values. Student’s t test or Mann–Whitney U test was applied to analyze continuous variables and calculate P values. To set up a multivariate regression analysis model for investigating the potential risk factors for 30-day microbiological eradication, each variable was evaluated by univariate analysis at first. Variables with P values ≤ 0.10 were added in the binary logistic regression analysis. The Kaplan–Meier method was chosen to achieve the survival analysis. Any variable with P value ≤ 0.10 was involved in a Cox proportional hazards regression model with a forward stepwise selection for analyzing 30-day mortality, while only variables with P values ≤ 0.10 remained in this model. The differences of variables between CAZ/AVI group and PMB group were compared in advance. Variables with P values ≤ 0.20 were included in both binary and Cox proportional hazards regression analysis, with the purpose of adjusting for confounding by indication. Covariates with P values ≤ 0.10 were kept in the models. Furthermore, a propensity score for the CAZ/AVI group was calculated by a logistic regression model covering the aforementioned variables with P values ≤ 0.20 and included in these two regression models. The plot of log [−log(survival)] versus log(time) was utilized to evaluate the proportional hazard assumption graphically. The collinearity between covariates was also checked. Tests for interactions were not performed. All tests were two-tailed, and P values ≤ 0.05 were considered statistically significant.
Discussion
Nowadays, among the available antimicrobial agents against CRKP infection, novel β-lactam/β-lactamase inhibitors (BL/BLIs) are the mainstay of effective pharmacotherapeutic schemes for CRKP-infected patients. It is recommended that CAZ/AVI is the preferred therapeutic agent against multiple sources of CRKP infection, according to the clinical guidance from Infectious Diseases Society of America (IDSA) [
21]. In China, there were few effective drugs against CRKP, while CAZ/AVI was the only novel BL/BLI approved in clinical use, and PMB was also utilized in recent years as well.
Several in vitro and in vivo studies have discussed the effectiveness of CAZ/AVI and PMB, which proved that these two agents were both reliable treatment options for patients with CRKP infections [
22‐
25]. Fang et al. drew the conclusion that CAZ/AVI-based therapy was more effective than PMB-based therapy in treating CRKP infection by implementing a retrospective analysis to compare the efficacy between these two therapies [
5]. Nevertheless, suitable therapeutic combined agents with CAZ/AVI remain unclear. Further safety evaluation of CAZ/AVI and PMB should also be performed. It is reasonable for us to design a novel clinical trial to make a comprehensive analysis about effectiveness and safety of CAZ/AVI-based and PMB-based therapeutic schemes.
In this study, we evaluated the 30-day all-cause mortality as primary outcome of clinical efficacy between these two therapies. Patients with a higher age (> 65 years), suffering from sepsis, receiving CRRT during antimicrobial treatment, or having comorbidity of organ transplantation had a significantly higher risk of death.
CRRT is a negative factor for survival in our study, which is contrary to our knowledge that CRRT is widely used in critically ill patients since it plays a crucial role in elimination of inflammatory mediators and continuous control of hemodynamic and electrolytical stability in vivo. This phenomenon might have possible causes in two respects. On the one hand, surviving patients have better renal function than patients in the dead group according to the comparison of CrCl, which is related to a lower demand on CRRT. On the other hand, some clinical studies indicate that CRRT might not be beneficial to effectively lower mortality for infected patients or those with sepsis [
26,
27]. Regarding the controversy over CRRT in the current study, we should put too great an emphasis on kidney function to exclude the interference of utilizing CRRT in our further studies.
As for the treatment duration, we advocate that more than a 7-day antimicrobial treatment period might have a positive correlation with survival rate for CRKP-infected patients. Zhou et al.’s research revealed that a short duration of antimicrobial therapy from 4 to 9 days would significantly increase the mortality, which provided a strongly support for our result [
28]. However, we could not ignore the fact that patients may die within the 7-day duration of antimicrobial therapy. In the current study, the majority of patients (87.6%) received antimicrobial treatment for over 7 days, which implied that our conclusion might be convincing but still requires further verification. Moreover, it is acknowledged that appropriate treatment duration of infection is influenced by multiple factors, such as various sources of infection, severity of infection, immune status, and general response to therapy [
21,
29,
30]. Our conclusion on treatment duration might be too general to play an important role in clinical practice. Individualized therapy duration with different types of CRKP infection should be investigated case by case.
A gender-dependent difference also exists in our study. This is evidently important for patients with infection and sepsis, which is attributed to sex hormones specifically. Female patients with sepsis may have a survival benefit in comparison with male patients owing to the salutary effects of estrogen on releasing cytokines which could improve the positive immune response and restoring organ function after sepsis. The immunosuppressive role of testosterone is also associated with the higher mortality rate for male patients with infection [
31‐
33]. It is meaningful to investigate the mortality risk by using clinically accurate preclinical models that reflect sex differences in our further research.
CAZ/AVI-based therapy was proved to be apparently effective in treating patients with CRKP in the current study, not only improving survival rate but also increasing bacterial clearance rate, compared with PMB-based therapy. Quite a few studies demonstrate the great value of CAZ/AVI in treating CRKP infection. CAZ/AVI therapy was more clinically advantageous than other antibiotics to decrease 30-day mortality for patients with CRKP infection, according to Gu et al.’s study [
34]. Chen et al. analyzed CRKP-infected patients after liver transplantation retrospectively and summarized that no matter whether CAZ/AVI-based combination therapy or monotherapy was used, promising clinical efficacy and safety were revealed in treating severe CRKP infections [
35].
It is worth noting that CAZ/AVI resistance was observed in very few CRKP strains in our study, although none of the patients with CAZ/AVI-resistant CRKP infection were prescribed CAZ/AVI. Shields et al. found that both pneumonia and prior use of CRRT were risk factors for the development of CAZ/AVI resistance, which could possibly induce microbiological failure or mortality [
36]. Hence, potential risk of clinical failure should be a concern when clinicians prescribe CAZ/AVI as empirical therapy to treat CRKP-infected patients.
According to the result from subgroup analysis, we have recognized that CAZ/AVI combination therapies with tigecycline or amikacin showed notable differences in lowering 30-day mortality, compared to other therapeutic schemes. Tigecycline was identified as a notably effective combined agent in our previous study [
12]. Ojdana et al. undertook one in vitro research study to explore the synergy of antibiotics combination against CRKP. They found that a combination with CAZ/AVI and tigecycline was capable of exerting synergistic effects against CRKP [
37]. Another in vitro time-kill experiment demonstrated that combinations of CZA/AVI with both tigecycline and amikacin exhibited better antimicrobial effects than monotherapy [
38]. These two drugs could enhance the therapeutic efficiency against CRKP in terms of Chen et al.’s study [
25]. Nevertheless, we should point out that better therapeutic outcome was observed when using tigecycline and amikacin to treat pneumonia and urinary tract infection, in view of the pharmacokinetic/pharmacodynamic (PK/PD) characteristics of these two antibiotics, respectively. Since a fraction of patients suffered from the two aforementioned types of infection in the CAZ/AVI group (46.3% with respiratory infection and 14.1% with urinary tract infection), one needs to confirm if tigecycline and amikacin show sufficient clinical efficacy in other types of infection.
The results from our study also showed that patients who received CAZ/AVI-based antimicrobial therapy would have a significantly higher probability of CRKP clearance than those receiving PMB-based antimicrobial therapy in vivo, which was consistent with the conclusion of Fang et al.’s article [
5].
The safety analysis of corresponding laboratory parameters and AEs was conducted to verify the safety of these two therapeutic schemes as well. Generally speaking, we could conclude that safety could be ensured if patients receive CAZ/AVI or PMB therapeutic regimens since diarrhea was the most common AE during the treatment period in both cohorts and no severe AE was observed in the present study. A large study evaluating the safety of CAZ/AVI with the pooled data from seven phase II and III clinical studies elaborated that the incidence of CAZ/AVI-induced diarrhea varied from 3.1% to 15.4%, which was similar to our result [
39].
Significant augmentation of AST and ALT values was found during the CAZ/AVI treatment period in our study, which is consistent with statistical data from Cheng et al.’s study [
39]. This could be attributed to ceftazidime-induced transient elevations in hepatic enzymes [
40‐
42]. We should attach great importance to monitoring when using CAZ/AVI-based therapeutic treatment, especially in combination with drugs having verified hepatoxicity.
It is inevitable to discuss the controversy of the predominant AE of PMB, namely PMB-associated nephrotoxicity [
43]. Although PMB showed adequate efficacy against CRKP, it is not highly recommended for the treatment CRKP infections, because of its nephrotoxicity [
21]. Polymyxin-associated acute kidney injury (AKI) has a high incidence ranging from 10% to 60%, which is mainly ascribed to receipt of concomitant nephrotoxic agents and selection of inappropriate dose regimens [
14,
44,
45]. One must be cautious of PMB-induced AKI, while a significant decline of CrCl and BUN was found during PMB treatment duration.
The current study achieves both clinical efficacy and safety comparison between CAZ/AVI-based and PMB-based therapeutic regimen in critically ill CRKP-infected patients for the first time. We have tried our utmost to control the potential for indication bias in this study. On the one hand, variables which related to the potential difference between CAZ/AVI-based and PMB-based treatment were all evaluated in the multivariate model. On the other hand, the propensity scores were calculated and incorporated into regression analysis, which did not alter any variable in the final multivariate and Cox regression models. In summary, we maintain that our study is convincing because the indication bias could barely affect our investigation result.
The present study had some limitations. First of all, our investigation was a retrospective observational cohort study with insufficient participants, which could not exclude the indication biases. More well-designed clinical trials with a larger number of eligible patients should be performed to validate our conclusion in the future. Secondly, genotypic identification of carbapenemases for all clinical isolates of CRKP was not performed in the present study because we lacked the essential equipment and experimental reagents. Thirdly, therapeutic drug monitoring (TDM) was not utilized to evaluate PMB serum concentration, which might cause treatment failure or increase the risk of AKI due to subtherapeutic or excessive dose, respectively. Last but not the least, in order to lower 30-day mortality, appropriate antimicrobial therapy should be initiated within 24 h after the collection of microbiological cultures [
46]. However, the exact time to appropriate antimicrobial therapy for each patient was not collected in our study. We should include this variable in our future investigation.
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