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Erschienen in:
01.04.2010 | Original Research Paper
Fucoidan increases TNF-α-induced MMP-9 secretion in monocytic cell line U937
Erschienen in: Inflammation Research | Ausgabe 4/2010
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Objective
To investigate the effect of fucoidan on the expression of matrix metalloproteinase-9 (MMP-9) from monocytes.
Methods
Human monocytic cell line U937 was purchased from ATCC. During the experiment, FBS-free 1640 was used and U937 was cultivated with 20 ng/ml TNF-α and/or different concentrations of fucoidan for 24 h. RT-PCR experiments were used to determine the MMP-9 mRNA expression. ELISA and gelatin zymography detected MMP-9 amounts and activity in the supernatant. The intracellular level of MMP-9 was assayed by Western blot, and the level of CD44 on the surface was assayed by FACS.
Results
In this study, we showed that pro-inflammatory cytokine TNF-α up-regulated U937 MMP-9 mRNA and protein levels (P < 0.05). Fucoidan can increase the TNF-α-induced MMP-9 secretion from U937 (P < 0.05), but no significant difference was observed in MMP-9 mRNA. The intracellular level of MMP-9 treated with TNF-α and fucoidan was lower (P < 0.05) than that treated with TNF-α alone. In addition, we demonstrated that fucoidan downregulated the surface level of CD44, the main molecule to which MMP-9 attaches.
Conclusions
We demonstrated that fucoidan post-translationally regulated MMP-9 secretion from U937. Reduced intracellular level and decreased membrane attachment may contribute to the increase in MMP-9 secretion.