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01.08.2019 | Original Research Paper

Immunoexpression of canonical Wnt and NF-κB signaling pathways in the temporomandibular joint of arthritic rats

verfasst von: Luane Macêdo de Sousa, Joana Maria dos Santos Alves, Conceição da Silva Martins, Karuza Maria Alves Pereira, Paula Goes, Delane Viana Gondim

Erschienen in: Inflammation Research | Ausgabe 10/2019

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Abstract

Objective

To investigate the participation of canonical Wnt and NF-κB signaling pathways in an experimental model of chronic arthritis induced by methylated bovine serum albumin (mBSA) in rat temporomandibular joint (TMJ).

Materials and methods

Wistar rats were sensitized by mBSA+Complete Freund Adjuvant (CFA)/Incomplete Freund Adjuvant (IFA) on the first 14 days (1 ×/week). Subsequently, they received 1, 2 or 3 mBSA or saline solution injections into the TMJ (1 ×/week). Hypernociceptive threshold was assessed during the whole experimental period. 24 h after the mBSA injections, the TMJs were removed for histopathological and immunohistochemical analyses for TNF-α, IL-1β, NF-κB, RANKL, Wnt-10b, β-catenin and DKK1.

Results

The nociceptive threshold was significantly reduced after mBSA injections. An inflammatory infiltrate and thickening of the synovial membrane were observed only after mBSA booster injections. Immunolabeling of TNF-α, IL-1β and Wnt-10b was increased in the synovial membrane in arthritic groups. The immunoexpression of nuclear β-catenin was significantly higher only in the group that received 2 booster TMJ injections. However, NF-κB, RANKL and DKK1 immunoexpression were increased only in animals with 3 mBSA intra-articular injections.

Conclusion

Our results suggest that canonical Wnt and NF-κB signaling pathways participate in the hypernociception and inflammatory response in TMJ synovial membrane during the development of rheumatoid arthritis in rats.

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Titel: Immunoexpression of canonical Wnt and NF-κB signaling pathways in the temporomandibular joint of arthritic rats
verfasst von: Luane Macêdo de Sousa
Joana Maria dos Santos Alves
Conceição da Silva Martins
Karuza Maria Alves Pereira
Paula Goes
Delane Viana Gondim
Publikationsdatum: 01.08.2019
Verlag: Springer International Publishing
Erschienen in: Inflammation Research / Ausgabe 10/2019
Print ISSN: 1023-3830
Elektronische ISSN: 1420-908X
DOI: https://doi.org/10.1007/s00011-019-01274-4

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Immunoexpression of canonical Wnt and NF-κB signaling pathways in the temporomandibular joint of arthritic rats