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01.05.2008 | Brief Report

Ficoll-separated mononuclear cells from sepsis patients are contaminated with granulocytes

verfasst von: Erica L. T. van den Akker, Carla C. Baan, Bart van den Berg, Henk Russcher, Koen Joosten, Anita C. S. Hokken-Koelega, Steven W. J. Lamberts, Jan W. Koper

Erschienen in: Intensive Care Medicine | Ausgabe 5/2008

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Abstract

Objective

To determine the cell content and purity of Ficoll-separated peripheral blood mononuclear cells and granulocyte isolates in sepsis patients compared to healthy controls.

Design and setting

Prospective study in the adult and pediatric intensive care departments of the Erasmus University Medical Center in the Netherlands.

Patients

Three sepsis patients (two adults, one child) and four healthy controls.

Measurements and results

Blood leukocytes were separated by Ficoll into an interface and a bottom fraction. The cell content and purity was analyzed by cytospin and flow-cytometric immunofluorescence. In sepsis patients, the interface consisted of 11–52% mononuclear cells only, due to high contamination with granulocytes (48–89%). This was in contrast to a high proportion of mononuclear cells (88–100%) in healthy controls. The bottom fraction showed a cell purity of ≥ 92% polymorphonuclear granulocytes in sepsis patients as well as in healthy controls.

Conclusions

Ficoll-separated leukocytes of sepsis patients are not suitable for studying mononuclear cells but can be used for studying granulocytes with high purity. The mononuclear cell fraction is highly contaminated with granulocytes. Additional separation techniques are necessary to obtain a pure cell fraction.

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Titel: Ficoll-separated mononuclear cells from sepsis patients are contaminated with granulocytes
verfasst von: Erica L. T. van den Akker
Carla C. Baan
Bart van den Berg
Henk Russcher
Koen Joosten
Anita C. S. Hokken-Koelega
Steven W. J. Lamberts
Jan W. Koper
Publikationsdatum: 01.05.2008
Verlag: Springer-Verlag
Erschienen in: Intensive Care Medicine / Ausgabe 5/2008
Print ISSN: 0342-4642
Elektronische ISSN: 1432-1238
DOI: https://doi.org/10.1007/s00134-007-0989-0

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Ficoll-separated mononuclear cells from sepsis patients are contaminated with granulocytes

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Objective

Design and setting

Patients

Measurements and results

Conclusions

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Objective

Design and setting

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Measurements and results

Conclusions

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