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Erschienen in:
01.01.2009 | Functional Neuroradiology
Absolute choline concentration measured by quantitative proton MR spectroscopy correlates with cell density in meningioma
Erschienen in: Neuroradiology | Ausgabe 1/2009
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Introduction
This study was aimed to investigate the relationship between quantitative proton magnetic resonance spectroscopy (1H-MRS) and pathological changes in meningioma.
Materials and methods
Twenty-two meningioma cases underwent single voxel 1H-MRS (point-resolved spectroscopy sequence, repetition time/echo time = 2,000 ms/68, 136, 272 ms). Absolute choline (Cho) concentration was calculated using tissue water as the internal reference and corrected according to intra-voxel cystic/necrotic parts. Pathological specimens were stained with MIB-1 antibody to measure cell density and proliferation index. Correlation analysis was performed between absolute Cho concentration and cell density and MIB-1 labeled proliferation index.
Results
Average Cho concentration of all meningiomas before correction was 2.95 ± 0.86 mmol/kg wet weight. It was increased to 3.23 ± 1.15 mmol/kg wet weight after correction. Average cell density of all meningiomas was 333 ± 119 cells/HPF, and average proliferation index was 2.93 ± 5.72%. A linear, positive correlation between cell density and Cho concentration was observed (r = 0.650, P = 0.001). After correction of Cho concentration, the correlation became more significant (r = 0.737, P < 0.001). However, no significant correlation between Cho concentration and proliferation index was found. There seemed to be a positive correlation trend after correction of Cho concentration but did not reach significant level.
Conclusion
Absolute Cho concentration, especially Cho concentration corrected according to intra-voxel cystic/necrotic parts, reflects cell density of meningioma.