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09.04.2020 | Original Article

Synchronous genitourinary lichen sclerosus signals a distinct urinary microbiome profile in men with urethral stricture disease

verfasst von: Andrew J. Cohen, Thomas W. Gaither, Sudarshan Srirangapatanam, Erick R. Castellanos, Anthony Enriquez, Kirkpatrick B. Fergus, Douglas Fadrosh, Susan Lynch, Nnenaya A. Mmonu, Benjamin N. Breyer

Erschienen in: World Journal of Urology | Ausgabe 2/2021

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Abstract

Purpose

Alterations in the urinary microbiome have been associated with urological diseases. The microbiome of patients with urethral stricture disease (USD) remains unknown. Our objective is to examine the microbiome of USD with a focus on inflammatory USD caused by lichen sclerosus (LS).

Methods

We collected mid-stream urine samples from men with LS-USD (cases; n = 22) and non-LS USD (controls; n = 76). DNA extraction, PCR amplification of the V4 hypervariable region of the 16S rRNA gene, and sequencing was done on the samples. Operational taxonomic units (OTUs) were defined using a > 97% sequence similarity threshold. Alpha diversity measurements of diversity, including microbiome richness (number of different OTUs) and evenness (distribution of OTUs) were calculated and compared. Microbiome beta diversity (difference between microbial communities) relationships with cases and controls were also assessed.

Results

Fifty specimens (13 cases and 37 controls) produced a 16S rRNA amplicon. Mean sample richness was 25.9 vs. 16.8 (p = 0.076) for LS-USD vs. non-LS USD, respectively. LS-USD had a unique profile of bacteria by taxonomic order including Bacillales, Bacteroidales and Pasteurellales enriched urine. The beta variation of observed bacterial communities was best explained by the richness.

Conclusions

Men with LS-USD may have a unique microbiologic richness, specifically inclusive of Bacillales, Bacteroidales and Pasteurellales enriched urine compared to those with non-LS USD. Further work will be required to elucidate the clinical relevance of these variations in the urinary microbiome.

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Titel: Synchronous genitourinary lichen sclerosus signals a distinct urinary microbiome profile in men with urethral stricture disease
verfasst von: Andrew J. Cohen
Thomas W. Gaither
Sudarshan Srirangapatanam
Erick R. Castellanos
Anthony Enriquez
Kirkpatrick B. Fergus
Douglas Fadrosh
Susan Lynch
Nnenaya A. Mmonu
Benjamin N. Breyer
Publikationsdatum: 09.04.2020
Verlag: Springer Berlin Heidelberg
Erschienen in: World Journal of Urology / Ausgabe 2/2021
Print ISSN: 0724-4983
Elektronische ISSN: 1433-8726
DOI: https://doi.org/10.1007/s00345-020-03198-9

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Synchronous genitourinary lichen sclerosus signals a distinct urinary microbiome profile in men with urethral stricture disease