nach oben

Archives of Dermatological Research

Erschienen in:

01.04.2010 | Original Paper

Gingko biloba extract reduces VEGF and CXCL-8/IL-8 levels in keratinocytes with cumulative effect with epigallocatechin-3-gallate

verfasst von: Sandra Trompezinski, Marlène Bonneville, Ingrid Pernet, Alain Denis, Daniel Schmitt, Jacqueline Viac

Erschienen in: Archives of Dermatological Research | Ausgabe 3/2010

Einloggen, um Zugang zu erhalten

Abstract

In skin inflammation, vascular endothelial growth factor (VEGF) and CXCL-8/IL-8 play an important role and are produced by activated keratinocytes. Extracts from Ginkgo biloba leaves (GBE), widely used in phytotherapy, have been reported to exert antioxidant and anti-inflammatory properties in the skin. We therefore evaluated the effects of GBE on the release of VEGF and CXCL8/IL-8 by normal human keratinocytes (NHKs) activated by tumor necrosis factor α (TNFα). Moreover, as we previously showed that epigallocatechin-3-gallate (EGCG) reduces VEGF and CXCL8/IL-8 secretion in TNFα-activated NHKs, we also tested its effect in association with GBE. Our results showed that GBE exerted a potent inhibition on VEGF and CXCL8/IL-8 levels in activated cells. In association with EGCG, GBE down-regulated VEGF and CXCL8/IL-8 levels in a cumulative manner in TNFα-stimulated NHKs. These results suggest that GBE, alone or in association with EGCG may contribute to moderate inflammatory processes in skin diseases associated with angiogenesis.

Baggiolini M, Dewald B, Moser B (1994) Interleukin-8 and related chemotactic cytokines—CXC and CC chemokines. Adv Immunol 55:97–179CrossRefPubMed

Ballaun C, Weninger W, Uthman A et al (1995) Human keratinocytes express the three major splice forms of vascular endothelial growth factor. J Invest Dermatol 104:7–10CrossRefPubMed

Castelli D, Colin L, Camel E et al (1998) Pretreatment of skin with a Ginkgo biloba extract/sodium carboxymethyl-beta-1, 3-glucan formulation appears to inhibit the elicitation of allergic contact dermatitis in man. Contact Dermat 38:123–126CrossRef

Cheung F, Yl Siow, Wz Chen et al (1999) Inhibitory effect of Ginkgo biloba extract on the expression of inducible nitric oxide synthase in endothelial cells. Biochem Pharmacol 58:1665–1673CrossRefPubMed

Defeudis FV, Papadopoulos V, Drieu K (2003) Ginkgo biloba extracts and cancer: a research area in its infancy. Fundam Clin Pharmacol 17:405–417CrossRefPubMed

Ferrara N, Davis-Smyth T (1997) The biology of vascular endothelial growth factor. Endocr Rev 18:4–25CrossRefPubMed

Frank S, Hubner G, Breier G et al (1995) Regulation of vascular endothelial growth factor expression in cultured keratinocytes. Implications for normal and impaired wound healing. J Biol Chem 270:12607–12613CrossRefPubMed

Fujiki H, Suganuma M, Okabe S et al (1999) Mechanistic findings of green tea as cancer preventive for humans. Proc Soc Exp Biol Med 220:225–228CrossRefPubMed

Gomaa AH, Yaar M, Eyada MM et al (2007) Lymphangiogenesis and angiogenesis in non-phymatous rosacea. J Cutan Pathol 34:748–753CrossRefPubMed

10.

Huh H, Staba E (1992) The botany and chemistry of Ginkgo biloba L. J Herbs Spices Med Plants 1:92–124CrossRef

11.

Juarez CP, Muino JC, Guglielmone H et al (2000) Experimental retinopathy of prematurity: angiostatic inhibition by nimodipine, ginkgo-biloba, and dipyridamole, and response to different growth factors. Eur J Ophthalmol 10:51–59PubMed

12.

Kanowski S, Herrmann WM, Stephan K et al (1996) Proof of efficacy of the ginkgo biloba special extract EGb 761 in outpatients suffering from mild to moderate primary degenerative dementia of the Alzheimer type or multi-infarct dementia. Pharmacopsychiatry 29:47–56CrossRefPubMed

13.

Katiyar SK, Ahmad N, Mukhtar H (2000) Green tea and skin. Arch Dermatol 136:989–994CrossRefPubMed

14.

Kim BH, Lee YS, Kang KS (2003) The mechanism of retinol-induced irritation and its application to anti-irritant development. Toxicol Lett 146:65–73CrossRefPubMed

15.

Kim SJ (2001) Effect of biflavones of Ginkgo biloba against UVB-induced cytotoxicity in vitro. J Dermatol 28:193–199PubMed

16.

Kwak WJ, Han CK, Son KH et al (2002) Effects of Ginkgetin from Ginkgo biloba Leaves on cyclooxygenases and in vivo skin inflammation. Planta Med 68:316–321CrossRefPubMed

17.

Larsen CG, Anderson AO, Oppenheim JJ et al (1989) Production of interleukin-8 by human dermal fibroblasts and keratinocytes in response to interleukin-1 or tumour necrosis factor. Immunology 68:31–36PubMed

18.

Lim H, Son KH, Chang HW et al (2006) Effects of anti-inflammatory biflavonoid, ginkgetin, on chronic skin inflammation. Biol Pharm Bull 29:1046–1049CrossRefPubMed

19.

Lim H, Son KH, Chang HW et al (2006) Inhibition of chronic skin inflammation by topical anti-inflammatory flavonoid preparation, Ato Formula. Arch Pharm Res 29:503–507CrossRefPubMed

20.

Longuet-Perret I, Schmitt D, Viac J (1998) Tumour necrosis factor-alpha is involved in the contrasting effects of ultraviolet B and ultraviolet A1 radiation on the release by normal human keratinocytes of vascular permeability factor. Br J Dermatol 138:221–224CrossRefPubMed

21.

Mahadevan S, Park Y (2008) Multifaceted therapeutic benefits of Ginkgo biloba L.: chemistry, efficacy, safety, and uses. J Food Sci 73:R14–R19PubMedCrossRef

22.

Mizuno M, Mt Droy-Lefaix, Packer L (1996) Gingko biloba extract EGb 761 is a suppresser of AP-1 transcription factor stimulated by phorbol 12-myristate 13-acetate. Biochem Mol Biol Int 39:395–401PubMed

23.

Neufeld G, Cohen T, Gengrinovitch S et al (1999) Vascular endothelial growth factor (VEGF) and its receptors. FASEB J 13:9–22PubMed

24.

Nishida S, Satoh H (2003) Mechanisms for the vasodilations induced by Ginkgo biloba extract and its main constituent, bilobalide, in rat aorta. Life Sci 72:2659–2667CrossRefPubMed

25.

Parsad D, Pandhi R, Juneja A (2003) Effectiveness of oral Ginkgo biloba in treating limited, slowly spreading vitiligo. Clin Exp Dermatol 28:285–287CrossRefPubMed

26.

Pernet I, Sagot V, Schmitt D et al (1999) UVA1 and UVB radiation but not PGE2 stimulate IL-8 release in normal human keratinocytes. Arch Dermatol Res 291:527–529CrossRefPubMed

27.

Pietrzak AT, Zalewska A, Chodorowska G et al (2008) Cytokines and anticytokines in psoriasis. Clin Chim Acta 394:7–21CrossRefPubMed

28.

Rimbach G, Gohil K, Matsugo S et al (2001) Induction of glutathione synthesis in human keratinocytes by Ginkgo biloba extract (EGb761). Biofactors 15:39–52CrossRefPubMed

29.

Rosenberger C, Solovan C, Rosenberger AD et al (2007) Upregulation of hypoxia-inducible factors in normal and psoriatic skin. J Invest Dermatol 127:2445–2452CrossRefPubMed

30.

Sagar SM, Yance D, Wong RK (2006) Natural health products that inhibit angiogenesis: a potential source for investigational new agents to treat cancer-Part 1. Curr Oncol 13:14–26PubMed

31.

Smith JR, Lanier VB, Braziel RM et al (2007) Expression of vascular endothelial growth factor and its receptors in rosacea. Br J Ophthalmol 91:226–229CrossRefPubMed

32.

Takematsu H, Tagami H (1993) Mode of release of interleukin-8 from proliferating human epidermal keratinocytes in vitro. Exp Dermatol 2:121–124CrossRefPubMed

33.

Trompezinski S, Denis A, Schmitt D et al (2003) Comparative effects of polyphenols from green tea (EGCG) and soybean (genistein) on VEGF and IL-8 release from normal human keratinocytes stimulated with the proinflammatory cytokine TNFalpha. Arch Dermatol Res 295:112–116CrossRefPubMed

34.

Trompezinski S, Pernet I, Schmitt D et al (2001) UV radiation and prostaglandin E2 up-regulate vascular endothelial growth factor (VEGF) in cultured human fibroblasts. Inflamm Res 50:422–427CrossRefPubMed

35.

Viac J, Palacio S, Schmitt D et al (1997) Expression of vascular endothelial growth factor in normal epidermis, epithelial tumors and cultured keratinocytes. Arch Dermatol Res 289:158–163CrossRefPubMed

36.

Wei Z, Peng Q, Bh Lau et al (1999) Ginkgo biloba inhibits hydrogen peroxide-induced activation of nuclear factor kappa B in vascular endothelial cells. Gen Pharmacol 33:369–375CrossRefPubMed

37.

Xia YP, Li B, Hylton D et al (2003) Transgenic delivery of VEGF to mouse skin leads to an inflammatory condition resembling human psoriasis. Blood 102:161–168CrossRefPubMed

38.

Yang PY, Rui YC, Zhang L et al (2002) Expression of vascular endothelial growth factor in U937 foam cells and the inhibitory effect of drugs. Yao Xue Xue Bao 37:86–89PubMed

39.

Yoshikawa T, Naito Y, Kondo M (1999) Ginkgo biloba leaf extract: review of biological actions and clinical applications. Antioxid Redox Signal 1:469–480CrossRefPubMed

40.

Zhang L, Rui YC, Yang PY et al (2002) Inhibitory effects of Ginkgo biloba extract on vascular endothelial growth factor in rat aortic endothelial cells. Acta Pharmacol Sin 23:919–923PubMed

41.

Zheng SX, Zhou LJ, Chen ZL et al (2000) Bilobalide promotes expression of glial cell line-derived neurotrophic factor and vascular endothelial growth factor in rat astrocytes. Acta Pharmacol Sin 21:151–155PubMed

Titel: Gingko biloba extract reduces VEGF and CXCL-8/IL-8 levels in keratinocytes with cumulative effect with epigallocatechin-3-gallate
verfasst von: Sandra Trompezinski
Marlène Bonneville
Ingrid Pernet
Alain Denis
Daniel Schmitt
Jacqueline Viac
Publikationsdatum: 01.04.2010
Verlag: Springer-Verlag
Erschienen in: Archives of Dermatological Research / Ausgabe 3/2010
Print ISSN: 0340-3696
Elektronische ISSN: 1432-069X
DOI: https://doi.org/10.1007/s00403-009-0979-x

Leitlinien kompakt für die Dermatologie

Mit medbee Pocketcards sicher entscheiden.

^{Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag}

Kostenlos registrieren

Neu im Fachgebiet Dermatologie

Hirsutismus bei PCOS: Laser- und Lichttherapien helfen

26.04.2024 Hirsutismus Nachrichten

Laser- und Lichtbehandlungen können bei Frauen mit polyzystischem Ovarialsyndrom (PCOS) den übermäßigen Haarwuchs verringern und das Wohlbefinden verbessern – bei alleiniger Anwendung oder in Kombination mit Medikamenten.

Bei schweren Reaktionen auf Insektenstiche empfiehlt sich eine spezifische Immuntherapie

25.04.2024 Allergien und Intoleranzreaktionen Nachrichten

Insektenstiche sind bei Erwachsenen die häufigsten Auslöser einer Anaphylaxie. Einen wirksamen Schutz vor schweren anaphylaktischen Reaktionen bietet die allergenspezifische Immuntherapie. Jedoch kommt sie noch viel zu selten zum Einsatz.

Auf diese Krankheiten bei Geflüchteten sollten Sie vorbereitet sein

22.04.2024 DGIM 2024 Nachrichten

Um Menschen nach der Flucht aus einem Krisengebiet bestmöglich medizinisch betreuen zu können, ist es gut zu wissen, welche Erkrankungen im jeweiligen Herkunftsland häufig sind. Dabei hilft eine Internetseite der CDC (Centers for Disease Control and Prevention).

Kein Abstrich bei chronischen Wunden ohne Entzündungszeichen!

16.04.2024 DGIM 2024 Nachrichten

Den Reflex, eine oberflächliche chronische Hautwunde ohne Entzündungszeichen in jedem Fall abzustreichen, sollte man nach einer neuen „Klug-entscheiden“-Empfehlung unterdrücken.

Update Dermatologie

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.

Newsletter bestellen

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 3/2010

The skin pathergy test: innately useful?

Platelet activating factor stimulates arachidonic acid release in differentiated keratinocytes via arachidonyl non-selective phospholipase A2

Rhodiola rosea ability to enrich cellular antioxidant defences of cultured human keratinocytes

Prunella vulgaris extract and rosmarinic acid prevent UVB-induced DNA damage and oxidative stress in HaCaT keratinocytes

Patented natural avocado sugars modulate the HBD-2 expression in human keratinocytes through the involvement of protein kinase C and protein tyrosine kinases