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12.09.2018 | Original Paper

The role of TrkA in the promoting wounding–healing effect of CD271 on epidermal stem cells

verfasst von: Min Zhang, Yuehou Zhang, Jun Ding, Xiaohong Li, Chengyu Zang, Siyuan Yin, Jiaxu Ma, Yibing Wang, Yongqian Cao

Erschienen in: Archives of Dermatological Research | Ausgabe 9/2018

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Abstract

CD271, a receptor of nerve growth factor (NGF), affects the biological properties of epidermal stem cells (eSCs) which are essential for skin wound closure. Tropomyosin-receptor kinase A (TrkA), another receptor of NGF, combined with CD271 has been involved with nervous system and skin keratinocytes. However, the exact role of TrkA combined with CD271 in eSCs during skin wound closure is still unclear. This study aimed to reveal the role of TrkA in the promoting wounding–healing effect of CD271 on eSCs. We obtained CD271-vo (over-expression of CD271) eSCs by lentiviral infection. K252a was used to inhibit TrkA expression. Full-thickness skin mouse wound closure model (5 mm in diameter) was used to detect the ability of CD271 over-expressed/TrkA-deficient during wound healing. The biological characteristics of eSCs and their proliferation and apoptosis were detected using immunohistochemistry and western blot. The expressions of protein kinase B (pAkt)/Akt, phosphorylated extracellular-signal-related kinase (pERK)/ERK1/2, and c-Jun N-terminal kinase (pJNK)/JNK were also detected by western blot. We found that over-expression of CD271 promoted the biological functions of eSCs. Interestingly, over-expression of CD271 in the absence of TrkA neither promoted eSCs’ migration and proliferation nor promoted wound healing in a mouse model. In addition, we observed the reduced expression of pAkt/Akt and pERK/ERK1/2 following TrkA inhibition in vitro. Our studies demonstrated that the role of TrkA in the promoting wounding–healing effect of CD271 on eSCs.

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Titel: The role of TrkA in the promoting wounding–healing effect of CD271 on epidermal stem cells
verfasst von: Min Zhang
Yuehou Zhang
Jun Ding
Xiaohong Li
Chengyu Zang
Siyuan Yin
Jiaxu Ma
Yibing Wang
Yongqian Cao
Publikationsdatum: 12.09.2018
Verlag: Springer Berlin Heidelberg
Erschienen in: Archives of Dermatological Research / Ausgabe 9/2018
Print ISSN: 0340-3696
Elektronische ISSN: 1432-069X
DOI: https://doi.org/10.1007/s00403-018-1863-3

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The role of TrkA in the promoting wounding–healing effect of CD271 on epidermal stem cells

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