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01.11.2012 | General Gynecology

Medical ozone therapy decreases postoperative uterine adhesion formation in rats

verfasst von: Bulent Uysal, Suzi Demirbag, Yavuz Poyrazoglu, Tuncer Cayci, Narter Yesildaglar, Ahmet Guven, Ilhami Sürer, Ahmet Korkmaz

Erschienen in: Archives of Gynecology and Obstetrics | Ausgabe 5/2012

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Abstract

Purpose

Various studies have been performed to find out novel treatment strategies to prevent postoperative adhesion formation. Ozone therapy (OT) is shown to reduce inflammation in several pathological conditions. Therefore, we aimed to evaluate the efficacy of OT in a rat model of experimental uterine adhesion (EUA).

Methods

Thirty female Wistar rats (200–250 g) were divided into three groups: sham, EUA and EUA+OT. EUA and EUA+OT groups were subjected to the postoperative adhesion procedure by bipolar coagulation on the uterine horns and corresponding pelvic sidewall parietal peritoneum. EUA+OT group received 0.7 mg/kg daily single dose for 3 days of ozone/oxygen mixture intraperitoneally after adhesion induction. All animals were killed on the 7th day and uterine adhesions were scored. Uterine tissues and peritoneal washing fluid were harvested for all analyses.

Results

Uterine malondialdehyde levels in the EUA group were significantly higher compared to the other groups. However, in the EUA group, uterine superoxide dismutase and glutathione peroxidase activities were lower than in other groups. Peritoneal fluid TNF-α levels were found to be significantly different for all groups (p < 0.001). Macroscopic total adhesion score was significantly higher in the EUA group compared to the other groups (p < 0.001). But, total score in the EUA+OT group was lower than in the EUA group (p = 0.006).

Conclusions

Medical OT prevents postoperative uterine adhesions by modulating TNF-α levels and oxidative/antioxidative status in an experimental uterine adhesion model.

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Titel: Medical ozone therapy decreases postoperative uterine adhesion formation in rats
verfasst von: Bulent Uysal
Suzi Demirbag
Yavuz Poyrazoglu
Tuncer Cayci
Narter Yesildaglar
Ahmet Guven
Ilhami Sürer
Ahmet Korkmaz
Publikationsdatum: 01.11.2012
Verlag: Springer-Verlag
Erschienen in: Archives of Gynecology and Obstetrics / Ausgabe 5/2012
Print ISSN: 0932-0067
Elektronische ISSN: 1432-0711
DOI: https://doi.org/10.1007/s00404-012-2435-y

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Medical ozone therapy decreases postoperative uterine adhesion formation in rats