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Archives of Gynecology and Obstetrics

Erschienen in:

01.04.2013 | Reproductive Medicine

Analysis of sperm telomere length in men with idiopathic infertility

verfasst von: J. Thilagavathi, M. Kumar, S. S. Mishra, S. Venkatesh, R. Kumar, R. Dada

Erschienen in: Archives of Gynecology and Obstetrics | Ausgabe 4/2013

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Abstract

Purpose

Telomeres are multifunctional nucleoprotein domains with hexanucleotide tandem repeat (5′ TTAGGG 3′) sequences, which cap the chromosome ends. However, the role of telomere and its length in sperm with regard to fertility remains unknown.

Methods

In this pilot study, we analyzed 32 idiopathic infertile men and 25 controls for sperm telomere length by quantitative polymerase chain reaction (Q-PCR), and correlated it with sperm DNA fragmentation index (DFI) and reactive oxygen species (ROS) levels.

Results

The relative sperm mean telomere length (T/S) of infertile men was found to be significantly lower (p < 0.005) when compared to controls (0.674 ± 0.028 vs. 0.699 ± 0.030). None of the sperm parameters such as sperm count, forward motility, morphology, ROS, and DFI were found to correlate with the sperm telomere length.

Conclusion

Shorter telomeres in sperm may be one of the causative factors responsible for male infertility, but further detailed studies are needed to confirm these findings.

Singh K, Jaiswal D (2011) Human male infertility: a complex multifactorial phenotype. Reprod Sci 18(5):418–425. doi:10.1177/1933719111398148 PubMedCrossRef

Venkatesh S, Thilagavathi J, Kumar K, Deka D, Talwar P, Dada R (2011) Cytogenetic, Y chromosome microdeletion, sperm chromatin and oxidative stress analysis in male partners of couples experiencing recurrent spontaneous abortions. Arch Gynecol Obstet 284(6):1577–1584. doi:10.1007/s00404-011-1990-y PubMedCrossRef

Shamsi MB, Venkatesh S, Pathak D, Deka D, Dada R (2011) Sperm DNA damage & oxidative stress in recurrent spontaneous abortion (RSA). Indian J Med Res 133(5):550–551 IndianJMedRes_2011_133_5_550_81674 [pii]PubMed

Thilagavathi J, Venkatesh S, Dada R (2012) Telomere length in reproduction. Andrologia (in press)

Treff NR, Su J, Taylor D, Scott RT Jr (2011) Telomere DNA deficiency is associated with development of human embryonic aneuploidy. PLoS Genet 7(6):e1002161. doi:10.1371/journal.pgen.1002161 PubMedCrossRef

Andrews NP, Fujii H, Goronzy JJ, Weyand CM (2010) Telomeres and immunological diseases of aging. Gerontology 56(4):390–403. doi:10.1159/000268620 PubMedCrossRef

Siderakis M, Tarsounas M (2007) Telomere regulation and function during meiosis. Chromosome Res 15(5):667–679. doi:10.1007/s10577-007-1149-7 PubMedCrossRef

Erenpreiss J, Spano M, Erenpreisa J, Bungum M, Giwercman A (2006) Sperm chromatin structure and male fertility: biological and clinical aspects. Asian J Androl 8(1):11–29. doi:10.1111/j.1745-7262.2006.00112.x PubMedCrossRef

Achi MV, Ravindranath N, Dym M (2000) Telomere length in male germ cells is inversely correlated with telomerase activity. Biol Reprod 63(2):591–598PubMedCrossRef

10.

Liu L, Blasco M, Trimarchi J, Keefe D (2002) An essential role for functional telomeres in mouse germ cells during fertilization and early development. Dev Biol 249(1):74–84 (S0012160602907359[pii])PubMedCrossRef

11.

Rodriguez S, Goyanes V, Segrelles E, Blasco M, Gosalvez J, Fernandez JL (2005) Critically short telomeres are associated with sperm DNA fragmentation. Fertil Steril 84(4):843–845. doi:10.1016/j.fertnstert.2005.05.014 PubMedCrossRef

12.

Moskovtsev SI, Willis J, White J, Mullen JB (2010) Disruption of telomere–telomere interactions associated with DNA damage in human spermatozoa. Syst Biol Reprod Med 56(6):407–412. doi:10.3109/19396368.2010.502587 PubMedCrossRef

13.

Scherthan H, Weich S, Schwegler H, Heyting C, Harle M, Cremer T (1996) Centromere and telomere movements during early meiotic prophase of mouse and man are associated with the onset of chromosome pairing. J Cell Biol 134(5):1109–1125PubMedCrossRef

14.

Calado RT, Young NS (2009) Telomere diseases. N Engl J Med 361(24):2353–2365. doi:10.1056/NEJMra0903373 PubMedCrossRef

15.

Venkatesh S, Riyaz AM, Shamsi MB, Kumar R, Gupta NP, Mittal S, Malhotra N, Sharma RK, Agarwal A, Dada R (2009) Clinical significance of reactive oxygen species in semen of infertile Indian men. Andrologia 41(4):251–256. doi:10.1111/j.1439-0272.2009.00943.x PubMedCrossRef

16.

Cawthon RM (2002) Telomere measurement by quantitative PCR. Nucleic Acids Res 30(10):e47PubMedCrossRef

17.

Gineitis AA, Zalenskaya IA, Yau PM, Bradbury EM, Zalensky AO (2000) Human sperm telomere-binding complex involves histone H2B and secures telomere membrane attachment. J Cell Biol 151(7):1591–1598PubMedCrossRef

18.

Hemann MT, Rudolph KL, Strong MA, DePinho RA, Chin L, Greider CW (2001) Telomere dysfunction triggers developmentally regulated germ cell apoptosis. Mol Biol Cell 12(7):2023–2030PubMed

19.

Santiso R, Tamayo M, Gosalvez J, Meseguer M, Garrido N, Fernandez JL (2010) Swim-up procedure selects spermatozoa with longer telomere length. Mutat Res 688(1–2):88–90. doi:10.1016/j.mrfmmm.2010.03.003 PubMed

Titel: Analysis of sperm telomere length in men with idiopathic infertility
verfasst von: J. Thilagavathi
M. Kumar
S. S. Mishra
S. Venkatesh
R. Kumar
R. Dada
Publikationsdatum: 01.04.2013
Verlag: Springer-Verlag
Erschienen in: Archives of Gynecology and Obstetrics / Ausgabe 4/2013
Print ISSN: 0932-0067
Elektronische ISSN: 1432-0711
DOI: https://doi.org/10.1007/s00404-012-2632-8

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Springer Medizin

Analysis of sperm telomere length in men with idiopathic infertility

Abstract

Purpose

Methods

Results

Conclusion

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Springer Medizin

Abstract

Purpose

Methods

Results

Conclusion

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