Erschienen in:
01.09.2014 | General Gynecology
Increased adhesion formation after gelatin–thrombin matrix application in a rat model
verfasst von:
Mete Çağlar, Ali Yavuzcan, Elif Yıldız, Bayram Yılmaz, Serdar Dilbaz, Selahattin Kumru
Erschienen in:
Archives of Gynecology and Obstetrics
|
Ausgabe 3/2014
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Abstract
Purpose
To evaluate the effects of gelatin matrix and thrombin-based hemostatic sealant agents (gelatin–thrombin matrix) on postoperative adhesion formation in the rat
uterine horn model.
Methods
A total of fourteen female Wistar-Albino rats were used in our postoperative
adhesion formation model. Both uterine horns were exposed by midline incision with
sterile technique and each uterine horn was traumatized by bipolar electrocautery.
Before closure of the abdomen, 0.9 % NaCl solution was administered intraperitoneally
in the control group and the gelatin–thrombin matrix was applied on the traumatized
areas on the uterine horns of the rats in the study group. At day 21 after the first
surgery, the intraperitoneal macroscopic adhesion scores and the extent of fibrosis,
inflammation scores, inflammatory cell activities, inflammation types of adhesion
tissues, and the level of free oxygen radicals [malondialdehyde (MDA)] and antioxidant
enzyme activity [superoxide dismutase (SOD) and glutathione S-transferase (GST)] in
the uterine horn tissue were measured.
Results
There was no statistically significant difference between the two groups in
terms of macroscopic adhesion scores (p = 0.064), MDA levels (p = 0.121), and GST
activity (p = 0.360). However, there was statistically significant difference between the
two groups in terms of the extent of fibrosis (p < 0.001), inflammation score (p = 0.001),
inflammatory cell activity (p = 0.002), and SOD activity (p < 0.001).
Conclusions
Gelatin–thrombin matrix was found to have increased the extent of
fibrosis, inflammatory cell activity, and inflammation score, and reduced the activity of
SOD, which acts to prevent cell damage.