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Graefe's Archive for Clinical and Experimental Ophthalmology
Erschienen in: Graefe's Archive for Clinical and Experimental Ophthalmology 7/2004

01.07.2004 | Laboratory Investigation

Subretinal electrical stimulation of the rabbit retina with acutely implanted electrode arrays

verfasst von: Florian Gekeler, Karin Kobuch, Hartmut Normann Schwahn, Alfred Stett, Kei Shinoda, Eberhart Zrenner

Erschienen in: Graefe's Archive for Clinical and Experimental Ophthalmology | Ausgabe 7/2004

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Abstract

Background

Subretinal implants intend to replace photoreceptor function in patients suffering from degenerative retinal disease by topically applying electrical stimuli from the subretinal space. This study intended to prove the feasibility of a newly developed transchoroidal surgery and, furthermore, of a subretinal electrode array, which closely resembles envisioned human implants to electrically stimulate the visual system in rabbits.

Methods

Five rabbits (ten eyes) were implanted with a 4×2-electrode array via a transchoroidal access to the subretinal space. The electrodes were connected to an arbitrary stimulus generator to apply voltage pulses. Retinae were accessed by light microscopy after stimulation with various intensities.

Results

The stimulating foil could be introduced into the subretinal space in all eyes. In seven of ten eyes electrically evoked cortical potentials following subretinal electrical stimulation could be elicited. Threshold voltages ranged from less than 0.1 to 2.38 V with a corresponding threshold charge of approximately 1.0 nC per electrode or 10 µC/cm2. Histology revealed localized retinal damage over some of the electrodes succeeding stimulation strengths of 2 V and consistent damage over all electrodes succeeding voltages of 3 V.

Conclusions

The study demonstrates the feasibility of the transchoroidal surgical access to place subretinal implants in rabbit eyes and provides proof of successful cortical activation following subretinal electrical stimulation by an electrode array envisioned for human implantations.
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Metadaten
Titel
Subretinal electrical stimulation of the rabbit retina with acutely implanted electrode arrays
verfasst von
Florian Gekeler
Karin Kobuch
Hartmut Normann Schwahn
Alfred Stett
Kei Shinoda
Eberhart Zrenner
Publikationsdatum
01.07.2004
Verlag
Springer-Verlag
Erschienen in
Graefe's Archive for Clinical and Experimental Ophthalmology / Ausgabe 7/2004
Print ISSN: 0721-832X
Elektronische ISSN: 1435-702X
DOI
https://doi.org/10.1007/s00417-004-0862-6

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