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Graefe's Archive for Clinical and Experimental Ophthalmology

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01.11.2012 | Retinal Disorders

Tempol protects against intravitreous indocyanine green-induced retinal damage in rats

verfasst von: Sebastian Thaler, Bogomil Voykov, Gabriel Willmann, Michal Fiedorowicz, Robert Rejdak, Florian Gekeler, C. Albrecht May, Andreas Schatz, Frank Schuettauf

Erschienen in: Graefe's Archive for Clinical and Experimental Ophthalmology | Ausgabe 11/2012

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Abstract

Purpose

Indocyanine green (ICG) has been widely used as a vital dye for macular surgery. However, ICG can be toxic to retinal cells. Here we evaluate whether tempol (4-hydroxy-2,2,6,6-tetramethylpiperidine-1-oxyl), a free radical scavenger, can protect against ICG-induced retinal damage in rats.

Methods

Brown Norway rats received intravitreal injections of ICG 0.5 % or BSS as controls. Tempol (20 mg/kg BW) or PBS as a control was administered intraperitoneally 24 h and 30 min before ICG and once daily for 7 consecutive days. Tempol was detected in the retina using electron paramagnetic resonance (EPR) spectroscopy. One week after ICG injections, the effects of tempol on retinal toxicity were assessed by retinal ganglion cell (RGC) back-labeling and by light microscopy. Electroretinography (ERG) was performed after 1 and 2 weeks.

Results

ICG administration reduced RGC numbers by 17 % (1,943 ± 45 vs. 2,342 ± 31 RGCs/mm²). Tempol treatment rescued RGCs in a significant manner (2,258 ± 36, p < 0.01) and diminished morphological changes detected by light microscopy. ICG-injected eyes showed a significant reduction of ERG potentials only in PBS-treated animals (V_max 530 ± 145 µV vs. 779 ± 179 µV, p = 0.0052), but not in the tempol-treated group.

Conclusions

Tempol significantly attenuates ICG-induced toxicity in rat retinas and may therefore be considered for further evaluation as accompanying treatment in ICG-assisted chromovitrectomy.

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Titel: Tempol protects against intravitreous indocyanine green-induced retinal damage in rats
verfasst von: Sebastian Thaler
Bogomil Voykov
Gabriel Willmann
Michal Fiedorowicz
Robert Rejdak
Florian Gekeler
C. Albrecht May
Andreas Schatz
Frank Schuettauf
Publikationsdatum: 01.11.2012
Verlag: Springer-Verlag
Erschienen in: Graefe's Archive for Clinical and Experimental Ophthalmology / Ausgabe 11/2012
Print ISSN: 0721-832X
Elektronische ISSN: 1435-702X
DOI: https://doi.org/10.1007/s00417-012-2000-1

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Tempol protects against intravitreous indocyanine green-induced retinal damage in rats

Abstract

Purpose

Methods

Results

Conclusions

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Conclusions

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